Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ENT638_RS18745 Genome accession   NC_009436
Coordinates   3939288..3940721 (+) Length   477 a.a.
NCBI ID   WP_015960619.1    Uniprot ID   A0A9J9L0T3
Organism   Enterobacter sp. 638     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3934288..3945721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENT638_RS18715 (Ent638_3632) - 3934856..3935833 (+) 978 WP_015960613.1 calcium/sodium antiporter -
  ENT638_RS18720 (Ent638_3633) kdsD 3935846..3936832 (+) 987 WP_015960614.1 arabinose-5-phosphate isomerase KdsD -
  ENT638_RS18725 (Ent638_3634) kdsC 3936847..3937413 (+) 567 WP_015960615.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ENT638_RS18730 (Ent638_3635) lptC 3937410..3937985 (+) 576 WP_015960616.1 LPS export ABC transporter periplasmic protein LptC -
  ENT638_RS18735 (Ent638_3636) lptA 3937954..3938508 (+) 555 WP_041689538.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ENT638_RS18740 (Ent638_3637) lptB 3938515..3939240 (+) 726 WP_015960618.1 LPS export ABC transporter ATP-binding protein -
  ENT638_RS18745 (Ent638_3638) rpoN/rpoN1 3939288..3940721 (+) 1434 WP_015960619.1 RNA polymerase factor sigma-54 Machinery gene
  ENT638_RS18750 (Ent638_3639) hpf 3940744..3941031 (+) 288 WP_015960620.1 ribosome hibernation promoting factor -
  ENT638_RS18755 (Ent638_3640) ptsN 3941114..3941605 (+) 492 WP_015960621.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ENT638_RS18760 (Ent638_3641) rapZ 3941651..3942505 (+) 855 WP_015960622.1 RNase adapter RapZ -
  ENT638_RS18765 (Ent638_3642) npr 3942502..3942774 (+) 273 WP_015960623.1 PTS phosphocarrier protein NPr -
  ENT638_RS18770 (Ent638_3643) mtgA 3942828..3943553 (-) 726 WP_015960624.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ENT638_RS18775 (Ent638_3644) elbB 3943550..3944203 (-) 654 WP_015960625.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54064.93 Da        Isoelectric Point: 4.3667

>NTDB_id=28002 ENT638_RS18745 WP_015960619.1 3939288..3940721(+) (rpoN/rpoN1) [Enterobacter sp. 638]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHEEVDTQEKQDTETLDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFTETDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDSEIELDEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPRLDEARLIISEHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQGFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=28002 ENT638_RS18745 WP_015960619.1 3939288..3940721(+) (rpoN/rpoN1) [Enterobacter sp. 638]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGTTGGCGATGACGCCACAGTTACAACAGGCGATCCGTCTGTTGCA
ACTGTCAACGTTAGAACTTCAGCAGGAACTGCAGCAAGCGCTGGACAGTAATCCGCTGCTGGAGCAAACCGATCTTCATG
AAGAGGTAGATACCCAGGAAAAGCAGGATACCGAAACGCTCGATACGGCAGATGCCCTTGAGCAAAAAGAGATGCCTGAC
GAGTTACCGCTAGATGCCAGCTGGGATGAAATTTACACCGCTGGAACCCCATCCGGAACGCGCGCTGATTATCAGGATGA
TGAGTTACCGGTTTATCAGGGTGAAACCACGCAGTCGCTACAGGATTACCTGATGTGGCAGGTGGAGTTGACCCCTTTCA
CCGAAACCGATCGCGCGATTGCCACTTCCATTGTCGATGCCGTGGATGACACCGGGTATTTAACGGTGACCCTGGACGAA
ATTCTGGAAAGCATGGGTGATAGCGAAATCGAACTCGACGAGATCGAAGCCGTTCTCAAGCGCATTCAACGCTTTGATCC
TGTCGGCGTCGCAGCAAAAGATCTGCGCGACTGTTTGCTGATCCAGCTTTCTCAGTTTGCAAAAGAGACGCCAAGACTCG
ACGAAGCGCGACTGATTATCAGCGAACATTTGGATTTGCTGGCGAATCATGATTTCCGCAGCCTGATGCGCGTGACGCGA
CTCAAAGAAGATGTACTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCCCGGCCGGGGCAATCGATCCAAACCGG
CGAACCCGAATATGTGATTCCTGACGTTCTGGTCCGAAAACACAACGGTCTCTGGACCGTAGAACTCAATTCCGACAGCA
TTCCCCGCCTGCAAATCAACCAGCAATACGCTTCTATGTGCACCAGTGCGCGGAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGCCTCGAAAGCCGAAATGATACGCTGTTACGCGTGAGTCGCTGCAT
TGTGGAACAGCAGCAAGGCTTTTTTGAGCAGGGCGAAGAATTTATGAAACCCATGGTTTTGGCAGATATTGCTCAAGCCG
TTGAGATGCATGAATCGACCATTTCGCGTGTGACCACGCAGAAGTATCTGCACAGCCCACGTGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGTCATGTGAATACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCAATTCGTGCTCTGGTGAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATCATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment