Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K10_RS02265 Genome accession   NZ_CP027747
Coordinates   509630..510682 (+) Length   350 a.a.
NCBI ID   WP_124339505.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain 66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504630..515682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K10_RS02255 (C4K10_0453) - 505582..506850 (+) 1269 WP_009046595.1 malic enzyme-like NAD(P)-binding protein -
  C4K10_RS02260 (C4K10_0454) - 506978..509413 (-) 2436 WP_164485464.1 penicillin-binding protein 1A -
  C4K10_RS02265 (C4K10_0456) pilM 509630..510682 (+) 1053 WP_124339505.1 pilus assembly protein PilM Machinery gene
  C4K10_RS02270 (C4K10_0457) - 510682..511248 (+) 567 WP_124339506.1 PilN domain-containing protein -
  C4K10_RS02275 (C4K10_0458) pilO 511245..511862 (+) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  C4K10_RS02280 (C4K10_0459) - 511859..512386 (+) 528 WP_041985501.1 pilus assembly protein PilP -
  C4K10_RS02285 (C4K10_0460) pilQ 512400..514472 (+) 2073 WP_028683778.1 type IV pilus secretin PilQ Machinery gene
  C4K10_RS02290 (C4K10_0461) aroK 514477..514995 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37077.53 Da        Isoelectric Point: 5.0008

>NTDB_id=279961 C4K10_RS02265 WP_124339505.1 509630..510682(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain 66]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAS
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279961 C4K10_RS02265 WP_124339505.1 509630..510682(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain 66]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTTATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCTTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCAGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCTATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.682

100

0.56
  comM Acinetobacter nosocomialis M2

55.398

100

0.557
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment