Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   ECSMS35_RS20010 Genome accession   NC_010498
Coordinates   4049912..4050580 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli SMS-3-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4044912..4055580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECSMS35_RS19980 (EcSMS35_3967) rfaQ 4045090..4046148 (-) 1059 WP_000360395.1 lipopolysaccharide core heptosyltransferase RfaQ -
  ECSMS35_RS19985 (EcSMS35_3968) waaA 4046561..4047838 (+) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  ECSMS35_RS19990 (EcSMS35_3969) coaD 4047846..4048325 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  ECSMS35_RS19995 (EcSMS35_3970) mutM 4048364..4049173 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ECSMS35_RS20000 (EcSMS35_3971) rpmG 4049271..4049438 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  ECSMS35_RS20005 (EcSMS35_3972) rpmB 4049459..4049695 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  ECSMS35_RS20010 (EcSMS35_3973) radC 4049912..4050580 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  ECSMS35_RS20015 (EcSMS35_3974) coaBC 4050752..4051972 (+) 1221 WP_000050149.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ECSMS35_RS20020 (EcSMS35_3975) dut 4051950..4052408 (+) 459 WP_000976070.1 dUTP diphosphatase -
  ECSMS35_RS20025 (EcSMS35_3976) slmA 4052515..4053111 (+) 597 WP_000818598.1 nucleoid occlusion factor SlmA -
  ECSMS35_RS20030 (EcSMS35_3977) pyrE 4053148..4053789 (-) 642 WP_000781153.1 orotate phosphoribosyltransferase -
  ECSMS35_RS20035 (EcSMS35_3978) rph 4053855..4054571 (-) 717 WP_001247093.1 ribonuclease PH -
  ECSMS35_RS20040 (EcSMS35_3979) yicC 4054698..4055561 (+) 864 WP_000621328.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=27968 ECSMS35_RS20010 WP_001297375.1 4049912..4050580(-) (radC) [Escherichia coli SMS-3-5]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=27968 ECSMS35_RS20010 WP_001297375.1 4049912..4050580(-) (radC) [Escherichia coli SMS-3-5]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAGTTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTTACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAATGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment