Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   C4J87_RS05740 Genome accession   NZ_CP027734
Coordinates   1294071..1295129 (+) Length   352 a.a.
NCBI ID   WP_005785454.1    Uniprot ID   A0A0R3ALL9
Organism   Pseudomonas sp. R1-43-08     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1278839..1335226 1294071..1295129 within 0


Gene organization within MGE regions


Location: 1278839..1335226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J87_RS05635 (C4J87_1109) - 1278839..1279327 (-) 489 WP_124366018.1 Panacea domain-containing protein -
  C4J87_RS05640 (C4J87_1110) - 1279590..1280324 (+) 735 WP_124361528.1 XRE family transcriptional regulator -
  C4J87_RS05645 (C4J87_1113) - 1280914..1281252 (+) 339 WP_124366019.1 phage holin family protein -
  C4J87_RS05650 (C4J87_1114) - 1281272..1281787 (+) 516 WP_124366020.1 hypothetical protein -
  C4J87_RS05655 (C4J87_1115) - 1281791..1282399 (+) 609 WP_124366021.1 phage baseplate assembly protein V -
  C4J87_RS05660 (C4J87_1116) - 1282412..1282744 (+) 333 WP_124361531.1 phage baseplate protein -
  C4J87_RS05665 (C4J87_1117) - 1282741..1283736 (+) 996 WP_124366022.1 baseplate J/gp47 family protein -
  C4J87_RS05670 (C4J87_1118) - 1283733..1284371 (+) 639 WP_124366023.1 phage tail protein I -
  C4J87_RS05675 (C4J87_1119) - 1284368..1284901 (+) 534 WP_124366024.1 phage tail protein -
  C4J87_RS05680 (C4J87_1120) - 1284928..1285548 (+) 621 WP_124366025.1 hypothetical protein -
  C4J87_RS05685 (C4J87_1121) - 1285561..1285818 (+) 258 WP_124366026.1 tail fiber assembly protein -
  C4J87_RS26070 (C4J87_1122) - 1285905..1286078 (+) 174 WP_164484513.1 hypothetical protein -
  C4J87_RS05690 (C4J87_1123) - 1286081..1287247 (+) 1167 WP_124366027.1 phage tail protein -
  C4J87_RS05695 (C4J87_1124) - 1287247..1287753 (+) 507 WP_124366028.1 phage major tail tube protein -
  C4J87_RS05700 (C4J87_1125) - 1287806..1288387 (+) 582 WP_124366029.1 phage tail assembly protein -
  C4J87_RS05705 (C4J87_1127) - 1288516..1290738 (+) 2223 WP_124366030.1 phage tail tape measure protein -
  C4J87_RS05710 (C4J87_1128) - 1290738..1291121 (+) 384 WP_124366031.1 phage tail protein -
  C4J87_RS05715 (C4J87_1129) - 1291114..1291326 (+) 213 WP_124366032.1 tail protein X -
  C4J87_RS05720 (C4J87_1130) - 1291336..1292337 (+) 1002 WP_124366033.1 contractile injection system protein, VgrG/Pvc8 family -
  C4J87_RS05725 (C4J87_1131) - 1292360..1292917 (+) 558 WP_124361544.1 glycoside hydrolase family 19 protein -
  C4J87_RS05730 (C4J87_1132) - 1292905..1293405 (+) 501 WP_124366034.1 lysis system i-spanin subunit Rz -
  C4J87_RS05735 (C4J87_1133) - 1293487..1293987 (+) 501 WP_124366035.1 CinA family protein -
  C4J87_RS05740 (C4J87_1134) recA 1294071..1295129 (+) 1059 WP_005785454.1 recombinase RecA Machinery gene
  C4J87_RS05745 (C4J87_1135) recX 1295138..1295605 (+) 468 WP_124366036.1 recombination regulator RecX -
  C4J87_RS05750 (C4J87_1136) - 1295667..1296779 (-) 1113 WP_124366037.1 TIGR00730 family Rossman fold protein -
  C4J87_RS05755 (C4J87_1137) - 1297064..1297498 (-) 435 WP_124366038.1 PA3611 family quorum-sensing-regulated virulence factor -
  C4J87_RS05760 (C4J87_1138) - 1297677..1298396 (+) 720 WP_124366039.1 tRNA-uridine aminocarboxypropyltransferase -
  C4J87_RS05765 (C4J87_1140) erdR 1298648..1299298 (+) 651 WP_124366040.1 response regulator transcription factor ErdR -
  C4J87_RS05770 (C4J87_1141) - 1299374..1299736 (+) 363 WP_003189051.1 diacylglycerol kinase -
  C4J87_RS05775 (C4J87_1142) - 1299740..1300666 (-) 927 WP_010212678.1 LysR family transcriptional regulator -
  C4J87_RS05780 (C4J87_1143) fpr 1300791..1301570 (+) 780 WP_003189055.1 ferredoxin-NADP reductase -
  C4J87_RS05785 (C4J87_1144) tsaA 1301690..1302385 (-) 696 WP_124366041.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  C4J87_RS05790 (C4J87_1145) - 1302389..1302850 (-) 462 WP_124361554.1 DUF1456 family protein -
  C4J87_RS05795 (C4J87_1146) - 1302921..1303631 (-) 711 WP_124366042.1 rRNA pseudouridine synthase -
  C4J87_RS05800 (C4J87_1147) - 1303741..1304211 (-) 471 WP_124366043.1 GNAT family N-acetyltransferase -
  C4J87_RS05805 (C4J87_1148) rimO 1304312..1305637 (-) 1326 WP_124366044.1 30S ribosomal protein S12 methylthiotransferase RimO -
  C4J87_RS05810 (C4J87_1149) - 1305768..1306793 (+) 1026 WP_124366045.1 hypothetical protein -
  C4J87_RS05815 (C4J87_1150) - 1306790..1308409 (+) 1620 WP_124366046.1 recombinase family protein -
  C4J87_RS26590 (C4J87_1151) - 1308477..1308749 (+) 273 WP_124366047.1 DUF1654 domain-containing protein -
  C4J87_RS05825 (C4J87_1152) - 1308866..1309420 (+) 555 WP_124366048.1 hypothetical protein -
  C4J87_RS05830 (C4J87_1153) - 1309424..1309666 (+) 243 WP_124366049.1 hypothetical protein -
  C4J87_RS05835 (C4J87_1154) - 1309663..1309962 (+) 300 WP_122560722.1 hypothetical protein -
  C4J87_RS05840 (C4J87_1155) - 1309994..1310698 (-) 705 WP_038449107.1 XRE family transcriptional regulator -
  C4J87_RS05845 (C4J87_1156) - 1310849..1311079 (+) 231 WP_124366050.1 Cro/CI family transcriptional regulator -
  C4J87_RS05850 (C4J87_1157) - 1311076..1311699 (+) 624 WP_124366051.1 hypothetical protein -
  C4J87_RS05855 (C4J87_1158) - 1311705..1312631 (+) 927 WP_256679978.1 hypothetical protein -
  C4J87_RS05860 (C4J87_1159) - 1313335..1313595 (-) 261 WP_038449110.1 ogr/Delta-like zinc finger family protein -
  C4J87_RS05865 (C4J87_1160) - 1313668..1314537 (-) 870 WP_164487682.1 phage portal protein -
  C4J87_RS05870 (C4J87_1161) - 1314449..1316506 (-) 2058 WP_124366052.1 terminase large subunit domain-containing protein -
  C4J87_RS05875 (C4J87_1162) - 1316669..1317586 (+) 918 WP_124366053.1 GPO family capsid scaffolding protein -
  C4J87_RS05880 (C4J87_1163) - 1317588..1318607 (+) 1020 WP_124366054.1 phage major capsid protein, P2 family -
  C4J87_RS05885 (C4J87_1164) gpM 1318604..1319332 (+) 729 WP_124366055.1 phage terminase small subunit -
  C4J87_RS05890 (C4J87_1165) - 1319431..1319901 (+) 471 WP_124366056.1 head completion/stabilization protein -
  C4J87_RS05895 (C4J87_1166) - 1319898..1320347 (+) 450 WP_124366057.1 phage tail protein -
  C4J87_RS05900 (C4J87_1167) - 1320337..1321017 (+) 681 WP_124366058.1 virion morphogenesis protein -
  C4J87_RS05905 (C4J87_1168) - 1321029..1322150 (+) 1122 WP_124366059.1 DUF2586 domain-containing protein -
  C4J87_RS05910 (C4J87_1169) - 1322157..1322606 (+) 450 WP_032895736.1 phage protein -
  C4J87_RS05915 (C4J87_1170) - 1322606..1322824 (+) 219 WP_003235240.1 TraR/DksA family transcriptional regulator -
  C4J87_RS05920 (C4J87_1171) - 1322821..1323027 (+) 207 WP_057701077.1 hypothetical protein -
  C4J87_RS05925 (C4J87_1172) - 1323024..1323557 (+) 534 WP_124366060.1 lysozyme -
  C4J87_RS05930 (C4J87_1173) - 1323557..1324033 (+) 477 WP_124366061.1 lysis system i-spanin subunit Rz -
  C4J87_RS05935 (C4J87_1174) - 1324047..1324334 (+) 288 WP_124366062.1 putative phage tail assembly chaperone -
  C4J87_RS26315 (C4J87_1175) - 1324355..1324498 (+) 144 WP_032862709.1 hypothetical protein -
  C4J87_RS05940 (C4J87_1176) - 1324505..1326817 (+) 2313 WP_124366063.1 phage tail tape measure protein -
  C4J87_RS05945 (C4J87_1177) - 1326814..1327131 (+) 318 WP_124366064.1 DUF2590 family protein -
  C4J87_RS05950 (C4J87_1178) - 1327128..1328300 (+) 1173 WP_124366065.1 baseplate J/gp47 family protein -
  C4J87_RS05955 (C4J87_1179) - 1328297..1328821 (+) 525 WP_032895744.1 phage tail protein -
  C4J87_RS05960 (C4J87_1180) - 1328831..1331467 (+) 2637 WP_124366066.1 phage tail protein -
  C4J87_RS05965 (C4J87_1181) - 1331470..1332108 (+) 639 WP_124366067.1 hypothetical protein -
  C4J87_RS05970 (C4J87_1182) - 1332110..1333033 (+) 924 WP_124368940.1 hypothetical protein -
  C4J87_RS05975 (C4J87_1183) - 1333030..1333581 (+) 552 WP_124366068.1 DNA-binding protein -
  C4J87_RS05980 (C4J87_1184) - 1333578..1334282 (+) 705 WP_124366069.1 hypothetical protein -
  C4J87_RS05985 (C4J87_1185) - 1334255..1335226 (+) 972 WP_124366070.1 hypothetical protein -

Sequence


Protein


Download         Length: 352 a.a.        Molecular weight: 37624.19 Da        Isoelectric Point: 5.2997

>NTDB_id=279645 C4J87_RS05740 WP_005785454.1 1294071..1295129(+) (recA) [Pseudomonas sp. R1-43-08]
MDDNKKKALAAALGQIERQFGKGAVMRMGDHDRQAIPAISTGSLGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLSVIA
QAQKMGATCAFVDAEHALDPEYAGKLGVNVDDLLVSQPDTGEQALEITDMLVRSNAIDVIVVDSVAALVPKAEIEGEMGD
MHVGLQARLMSQALRKITGNIKNANCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRTGAVKEGDEVVGSE
TRVKVVKNKVAPPFRQAEFQILYGKGIYLNGEMIDLGVLHGFVEKSGAWYAYNGSKIGQGKANSAKFLADNPDIAATLEK
QIRDKLLTPAPDVKAAANREPVDEMEEADTDI

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=279645 C4J87_RS05740 WP_005785454.1 1294071..1295129(+) (recA) [Pseudomonas sp. R1-43-08]
ATGGACGACAACAAGAAGAAAGCCTTGGCTGCGGCCCTGGGTCAGATCGAACGTCAATTCGGCAAGGGTGCCGTAATGCG
TATGGGCGATCACGACCGTCAGGCAATCCCGGCTATCTCCACTGGCTCTCTGGGTCTGGATATCGCGCTCGGCATTGGCG
GCCTGCCAAAAGGCCGTATCGTTGAGATCTACGGTCCTGAATCTTCCGGTAAAACCACCCTGACCCTGTCGGTGATTGCC
CAGGCTCAGAAAATGGGCGCCACCTGCGCATTCGTCGATGCCGAGCACGCCCTGGATCCTGAATACGCCGGCAAGCTGGG
CGTCAACGTCGACGACCTGCTGGTTTCCCAGCCAGACACCGGTGAGCAAGCCCTGGAAATCACCGACATGCTGGTGCGCT
CCAACGCCATCGACGTGATCGTGGTCGACTCCGTGGCTGCCCTGGTACCCAAGGCAGAAATCGAAGGCGAAATGGGCGAC
ATGCACGTGGGCCTGCAAGCCCGCCTGATGTCCCAGGCGCTGCGTAAAATCACCGGCAACATCAAGAACGCCAACTGCCT
GGTGATCTTCATCAACCAGATCCGTATGAAAATCGGTGTGATGTTCGGCAGCCCGGAAACCACCACCGGTGGTAACGCGC
TGAAGTTCTACGCTTCGGTTCGTCTGGATATCCGTCGTACCGGTGCGGTGAAAGAAGGTGACGAGGTTGTCGGTAGCGAA
ACCCGCGTCAAAGTCGTGAAGAACAAGGTAGCCCCGCCTTTCCGCCAAGCAGAGTTCCAGATTCTTTATGGCAAAGGCAT
CTACCTGAACGGCGAGATGATCGACCTGGGCGTGCTGCACGGTTTTGTCGAGAAGTCCGGTGCCTGGTATGCCTACAACG
GCAGCAAGATCGGTCAGGGCAAGGCCAACTCGGCCAAGTTCCTGGCAGACAACCCGGATATCGCCGCCACGCTTGAAAAG
CAGATTCGCGACAAGCTGCTGACCCCTGCGCCAGACGTCAAAGCCGCCGCCAACCGTGAGCCGGTTGACGAGATGGAAGA
AGCCGACACTGACATCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0R3ALL9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Pseudomonas stutzeri DSM 10701

87.5

100

0.875

  recA Acinetobacter baylyi ADP1

74.64

98.58

0.736

  recA Acinetobacter baumannii D1279779

73.178

97.443

0.713

  recA Vibrio cholerae O1 biovar El Tor strain E7946

75.076

93.466

0.702

  recA Vibrio cholerae strain A1552

75.076

93.466

0.702

  recA Glaesserella parasuis strain SC1401

69.143

99.432

0.688

  recA Ralstonia pseudosolanacearum GMI1000

68.571

99.432

0.682

  recA Neisseria gonorrhoeae MS11

72.222

92.045

0.665

  recA Neisseria gonorrhoeae MS11

72.222

92.045

0.665

  recA Neisseria gonorrhoeae strain FA1090

72.222

92.045

0.665

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

64.526

92.898

0.599

  recA Helicobacter pylori strain NCTC11637

59.943

100

0.599

  recA Helicobacter pylori 26695

59.375

100

0.594

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

61.677

94.886

0.585

  recA Bacillus subtilis subsp. subtilis str. 168

61.682

91.193

0.562

  recA Streptococcus mutans UA159

60

92.33

0.554

  recA Streptococcus pyogenes NZ131

58.232

93.182

0.543

  recA Streptococcus pneumoniae Rx1

58.589

92.614

0.543

  recA Streptococcus pneumoniae D39

58.589

92.614

0.543

  recA Streptococcus pneumoniae R6

58.589

92.614

0.543

  recA Streptococcus pneumoniae TIGR4

58.589

92.614

0.543

  recA Streptococcus mitis SK321

58.824

91.761

0.54

  recA Streptococcus mitis NCTC 12261

58.824

91.761

0.54

  recA Latilactobacillus sakei subsp. sakei 23K

58.879

91.193

0.537

  recA Lactococcus lactis subsp. cremoris KW2

57.276

91.761

0.526

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.79

92.045

0.523


Multiple sequence alignment