Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C4J98_RS22995 Genome accession   NZ_CP027723
Coordinates   5079913..5081280 (-) Length   455 a.a.
NCBI ID   WP_124434094.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain 8B     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5074913..5086280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J98_RS22975 (C4J98_4577) - 5076274..5076642 (+) 369 WP_124434090.1 PilZ domain-containing protein -
  C4J98_RS22980 (C4J98_4579) - 5076989..5078134 (+) 1146 WP_124434091.1 mandelate racemase/muconate lactonizing enzyme family protein -
  C4J98_RS22985 (C4J98_4580) - 5078160..5079089 (+) 930 WP_124434092.1 LeuA family protein -
  C4J98_RS22990 (C4J98_4581) - 5079128..5079874 (+) 747 WP_124434093.1 sulfite exporter TauE/SafE family protein -
  C4J98_RS22995 (C4J98_4582) radA 5079913..5081280 (-) 1368 WP_124434094.1 DNA repair protein RadA Machinery gene
  C4J98_RS23000 (C4J98_4583) - 5081316..5081864 (-) 549 WP_124425054.1 ankyrin repeat domain-containing protein -
  C4J98_RS23005 (C4J98_4584) katB 5082003..5083544 (-) 1542 WP_124434095.1 catalase KatB -
  C4J98_RS23010 (C4J98_4585) mscL 5083832..5084248 (+) 417 WP_124434096.1 large-conductance mechanosensitive channel protein MscL -
  C4J98_RS23015 (C4J98_4586) - 5084292..5085068 (-) 777 WP_124359896.1 ferredoxin--NADP reductase -
  C4J98_RS23020 (C4J98_4587) - 5085497..5086207 (+) 711 WP_124434097.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48713.20 Da        Isoelectric Point: 6.8987

>NTDB_id=279390 C4J98_RS22995 WP_124434094.1 5079913..5081280(-) (radA) [Pseudomonas orientalis strain 8B]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLTETMIESGGAAAPTGRAGWTGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCSIASRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIESIIA
TARIEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFAMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGIQIIGVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=279390 C4J98_RS22995 WP_124434094.1 5079913..5081280(-) (radA) [Pseudomonas orientalis strain 8B]
ATGGCAAAGGCCAAGCGCATGTACGGCTGCACGGAGTGCGGCGCGACCTTTCCCAAATGGGCGGGCCAGTGCGGCGAGTG
CGGTGCGTGGAACACCCTGACTGAAACCATGATTGAAAGCGGTGGCGCCGCAGCGCCCACCGGCCGCGCCGGTTGGACCG
GGCAACAAGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTCGAAGAAATCCCGCGTTTCTCCACGGCCTCCGGCGAGCTG
GACCGGGTATTGGGCGGTGGCCTGGTGGATGGCTCCGTGGTGCTGATCGGCGGTGACCCGGGCATCGGTAAATCCACGAT
CCTGCTGCAGACCCTGTGCAGCATCGCCAGCCGCATGCCGGCGCTGTACGTCACCGGCGAAGAGTCCCAGCAACAGGTGG
CCATGCGCGCCCGTCGCCTGGGCCTGCCCCAGGACCAACTGCGGGTCATGACCGAAACCTGCATCGAAAGCATCATCGCC
ACGGCGCGCATCGAAAAACCCAAGGTCATGGTGATCGACTCGATCCAGACGATTTTCACCGAGCAACTGCAATCGGCGCC
CGGCGGCGTATCCCAAGTGCGCGAGAGTGCGGCGCTGTTGGTGCGCTATGCCAAGCAGAGCGGCACGGCGATCTTCCTGG
TCGGCCACGTGACCAAGGAAGGCGCGCTGGCCGGGCCGCGCGTGCTGGAACACATGGTGGATACCGTGCTGTATTTCGAA
GGCGAATCCGATGGTCGCCTGCGGTTGCTGCGGGCGGTGAAGAACCGTTTTGGCGCGGTAAACGAGTTGGGTGTATTTGC
CATGACCGACCGCGGGCTGAAAGAAGTCTCCAATCCGTCGGCGATCTTTCTCACCCGCGCCCAGGAAGAAGTCCCGGGCA
GCGTGGTGATGGCGACGTGGGAAGGCACCCGGCCGATGCTGGTGGAAGTCCAGGCGCTGGTGGATGACAGCCATCTGGCC
AACCCGCGCCGGGTCACCCTCGGCCTGGACCAGAACCGCCTGGCCATGCTGTTGGCGGTATTGCACCGCCATGGCGGCAT
TCCGACTCACGATCAGGACGTCTTCCTCAACGTGGTGGGCGGGGTCAAGGTGCTGGAGACCGCGTCCGACCTGGCGTTGA
TGGCGGCGGTGATGTCCAGCCTGCGCAATCGGCCGTTGCCCCACGACCTGCTGGTGTTTGGCGAGGTCGGCCTGTCCGGC
GAAGTGCGCCCGGTGCCCAGTGGCCAGGAACGCCTCAAGGAAGCCGCCAAGCACGGCTTCAAACGCGCGATTGTGCCCAA
GGGCAACGCGCCGAAGGAAGCGCCGCCGGGGATACAGATTATTGGCGTGACGCGGTTGGAACAGGCGCTGGATGCACTTT
TCGAGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.672

100

0.499
  radA Streptococcus pneumoniae Rx1

46.957

100

0.475
  radA Streptococcus pneumoniae R6

46.957

100

0.475
  radA Streptococcus pneumoniae TIGR4

46.957

100

0.475
  radA Streptococcus pneumoniae D39

46.957

100

0.475
  radA Streptococcus mitis SK321

47.149

100

0.473
  radA Streptococcus mitis NCTC 12261

46.93

100

0.47

Multiple sequence alignment