Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PNUC_RS09995 Genome accession   NC_009379
Coordinates   2005842..2006732 (+) Length   296 a.a.
NCBI ID   WP_011903763.1    Uniprot ID   A4T076
Organism   Polynucleobacter asymbioticus QLW-P1DMWA-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2000842..2011732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNUC_RS09965 (Pnuc_1922) pth 2001050..2001640 (+) 591 WP_011903757.1 aminoacyl-tRNA hydrolase -
  PNUC_RS09970 (Pnuc_1923) - 2001646..2001912 (-) 267 WP_011903758.1 YfhL family 4Fe-4S dicluster ferredoxin -
  PNUC_RS09975 (Pnuc_1924) coaD 2001930..2002427 (-) 498 WP_011903759.1 pantetheine-phosphate adenylyltransferase -
  PNUC_RS09980 (Pnuc_1925) rsmD 2002464..2003054 (-) 591 WP_011903760.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PNUC_RS09985 (Pnuc_1926) - 2003074..2004411 (-) 1338 WP_011903761.1 pitrilysin family protein -
  PNUC_RS09990 (Pnuc_1927) - 2004411..2005778 (-) 1368 WP_011903762.1 pitrilysin family protein -
  PNUC_RS09995 (Pnuc_1928) pilA 2005842..2006732 (+) 891 WP_011903763.1 signal recognition particle-docking protein FtsY Machinery gene
  PNUC_RS10000 (Pnuc_1929) rpoH 2006825..2007754 (+) 930 WP_011903764.1 RNA polymerase sigma factor RpoH -
  PNUC_RS10005 (Pnuc_1930) - 2007776..2008366 (-) 591 WP_011903765.1 SCO family protein -
  PNUC_RS10010 (Pnuc_1931) cyoE 2008368..2009261 (-) 894 WP_011903766.1 heme o synthase -
  PNUC_RS10015 (Pnuc_1932) - 2009258..2010388 (-) 1131 WP_011903767.1 heme A synthase -
  PNUC_RS10020 (Pnuc_1933) - 2010388..2011044 (-) 657 WP_011903768.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31544.16 Da        Isoelectric Point: 6.9754

>NTDB_id=27929 PNUC_RS09995 WP_011903763.1 2005842..2006732(+) (pilA) [Polynucleobacter asymbioticus QLW-P1DMWA-1]
MFGLRKTLGSLFKTSKTDEAWFDTLEESLIQSDVGLPTTEQLIIKLRKAAKSEKASSPEELQALLIQEVSTLLGSLEPNP
NPLFTDQKIHIPEVWLVVGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQDGGDAAA
VAHDAIHAANSRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
LIVTKLDGTAKGGVICALAHTLKDEPKPAVLALGKGEGIDDLVPFTAHQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=27929 PNUC_RS09995 WP_011903763.1 2005842..2006732(+) (pilA) [Polynucleobacter asymbioticus QLW-P1DMWA-1]
ATGTTCGGCCTACGTAAAACCCTCGGATCCCTATTCAAAACCAGCAAGACGGATGAAGCTTGGTTTGATACTTTAGAAGA
GTCACTCATTCAAAGTGATGTGGGCCTCCCCACAACTGAACAATTGATTATCAAATTACGTAAAGCTGCAAAATCTGAAA
AAGCATCCAGCCCAGAAGAGCTCCAGGCACTCCTCATACAAGAAGTGAGTACCTTACTAGGTTCACTAGAACCAAATCCA
AACCCATTATTTACAGATCAGAAGATCCATATTCCCGAGGTTTGGTTGGTAGTAGGGGTGAATGGTGCAGGCAAAACCAC
CACTATTGGCAAGTTATGCAAGCTCTTTCAATCTCAAGGTAAATCGGTATTACTGGCAGCTGGCGACACTTTTAGGGCGG
CCGCTAGAAATCAACTTCAAGAATGGGGTGGCAGAAATCAAGTGGATGTCATCACTCAAGATGGTGGTGACGCAGCTGCC
GTAGCCCATGATGCAATTCATGCGGCCAACTCTCGCAAGAGTGACATCTTAATTATTGATACAGCGGGACGACTGGCTAC
CCAAGATCACCTCATGGAAGAGCTCAAAAAAGTGAAGCGGGTGATTGGCAAGGCGCTTCCTGGCGCCCCTCACCACACCC
TACTGGTACTAGATGGCAACACAGGACAGAATGGTTTAAGCCAGGTAAAGGCCTTTCATGCAGCCCTGGGACTTACAGGG
CTCATTGTTACTAAGTTAGATGGCACCGCCAAAGGGGGTGTCATTTGCGCCCTTGCTCACACTCTGAAAGATGAACCCAA
ACCGGCTGTTTTAGCCCTAGGCAAAGGTGAAGGAATTGATGATTTAGTGCCTTTCACAGCCCACCAGTATTCATCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(92-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4T076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.584

98.986

0.53

Multiple sequence alignment