Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ASA_RS01685 Genome accession   NC_009348
Coordinates   332759..334198 (+) Length   479 a.a.
NCBI ID   WP_005314410.1    Uniprot ID   A4SHX7
Organism   Aeromonas salmonicida subsp. salmonicida A449     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327759..339198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASA_RS01655 (ASA_0309) mlaF 328336..329136 (-) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -
  ASA_RS01660 (ASA_0310) - 329344..330339 (+) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  ASA_RS01665 (ASA_0311) kdsC 330339..330893 (+) 555 WP_005314419.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ASA_RS01670 (ASA_0312) lptC 330890..331450 (+) 561 WP_005314417.1 LPS export ABC transporter periplasmic protein LptC -
  ASA_RS01675 (ASA_0313) lptA 331437..331964 (+) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  ASA_RS01680 (ASA_0314) lptB 331968..332693 (+) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  ASA_RS01685 (ASA_0315) rpoN/rpoN1 332759..334198 (+) 1440 WP_005314410.1 RNA polymerase factor sigma-54 Machinery gene
  ASA_RS01690 (ASA_0316) hpf 334220..334507 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  ASA_RS01695 (ASA_0317) ptsN 334510..334956 (+) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ASA_RS01700 (ASA_0318) rapZ 334999..335865 (+) 867 WP_005314405.1 RNase adapter RapZ -
  ASA_RS01705 (ASA_0319) npr 335886..336158 (+) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  ASA_RS01710 (ASA_0320) - 336177..338091 (-) 1915 Protein_306 PRD domain-containing protein -
  ASA_RS01715 (ASA_0321) - 338195..338935 (-) 741 WP_005314402.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53962.92 Da        Isoelectric Point: 4.3718

>NTDB_id=27893 ASA_RS01685 WP_005314410.1 332759..334198(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida A449]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=27893 ASA_RS01685 WP_005314410.1 332759..334198(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida A449]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4SHX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484

Multiple sequence alignment