Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   C4K40_RS02225 Genome accession   NZ_CP027705
Coordinates   481220..483439 (-) Length   739 a.a.
NCBI ID   WP_053132480.1    Uniprot ID   A0A7X1PQF3
Organism   Pseudomonas sp. CMR5c     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476220..488439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K40_RS02205 (C4K40_0445) hslU 476468..477811 (-) 1344 WP_022639725.1 ATP-dependent protease ATPase subunit HslU -
  C4K40_RS02210 (C4K40_0446) hslV 477893..478423 (-) 531 WP_007968352.1 ATP-dependent protease subunit HslV -
  C4K40_RS02215 (C4K40_0447) - 478612..479319 (-) 708 WP_022639727.1 SPOR domain-containing protein -
  C4K40_RS02220 (C4K40_0448) argS 479321..481057 (-) 1737 WP_053132482.1 arginine--tRNA ligase -
  C4K40_RS02225 (C4K40_0449) priA 481220..483439 (-) 2220 WP_053132480.1 primosomal protein N' Machinery gene
  C4K40_RS02230 (C4K40_0450) rpmE 483704..483925 (+) 222 WP_011058815.1 50S ribosomal protein L31 -
  C4K40_RS02235 (C4K40_0451) - 483967..484752 (+) 786 WP_022639731.1 thermonuclease family protein -
  C4K40_RS02240 (C4K40_0452) - 484940..486208 (+) 1269 WP_022639732.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81939.38 Da        Isoelectric Point: 8.4271

>NTDB_id=278910 C4K40_RS02225 WP_053132480.1 481220..483439(-) (priA) [Pseudomonas sp. CMR5c]
MPDAILRLALPSPLRRLFDYRAPAGVLRAQLQPGMRLRVPFGRREMIGILVQVADHSEVPADKLKPALALLDPTPPLPAA
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAEARQERFWSIAPGARLDDPRIARAPRQRDALTTLAQHPHGVPHGLL
SKLMLSKDSLDLLLAKELVQVEVRRHAPGARHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQEDIPIVLGSATPSLESLHNAYTGRYGLLRMNERAGGAKPPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECERCDARMTVHQRSGELRCHHCGHVERVPRHC
PQCGKVDLRPVGAGTERAEERLGILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDEACSEAERLLAEQQSAGIELLGPVPAPMERRAGRYRAQLLLQANARAPLHRLLSSWLLVLEQ
MPGGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=278910 C4K40_RS02225 WP_053132480.1 481220..483439(-) (priA) [Pseudomonas sp. CMR5c]
GTGCCCGACGCCATCCTGCGCCTCGCCCTGCCCTCACCCCTGCGCCGCCTGTTCGACTATCGTGCCCCGGCCGGCGTGCT
GCGCGCGCAACTGCAGCCGGGCATGCGCCTGCGGGTGCCGTTCGGCCGTCGCGAGATGATCGGCATCCTGGTCCAGGTGG
CCGATCACAGTGAGGTGCCGGCGGACAAGCTCAAGCCGGCCCTGGCCCTGCTCGACCCCACGCCGCCGCTGCCGGCGGCC
TTGTTCAAGCTGTGCCTGTGGACCGCCCAGTACTACCAGCACAGCCTGGGGGATACCCTGAGCTGGGCGCTGCCGGTGCT
GCTGCGCCAGGGCGAACCGGCCGAGGCCCGCCAGGAGCGCTTCTGGTCGATAGCCCCCGGCGCCCGCCTTGACGATCCGC
GCATCGCCCGTGCCCCACGCCAGCGCGACGCCCTGACGACCCTGGCCCAGCACCCTCACGGCGTCCCCCACGGGTTGCTG
AGCAAGTTGATGTTGAGCAAGGACAGCCTGGACCTGCTGCTGGCCAAGGAGCTGGTCCAGGTGGAAGTGCGCAGGCATGC
CCCCGGGGCCCGCCACGAACACTGGCTGGCCCAGCCGGAACTGCCACTCAACAGCGAACAACGTGCCGCCTACGAGGCCA
TCCGCGCCGGATTCGACAGCTACCATGCCTTTCTCCTGGCAGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTCGAAGCTGGCAAGCAGGCCCTGGTGCTGATTCCGGAGATCAACCTCGGCCCCCAGACCCTGGC
GCGCTTCGAACAGCGTTTCAATGCGCGCATCGCCCTGCTGCACTCGGCGGTCAACGACCGCGAACGCCTGGACGCCTGGC
TGGCCGCCCGGGACGGCGAGGCCGACATCATCATCGGCACCCGTTCCGCGCTGTTCACGCCGATGAAGAATCCCGGCCTG
ATCATCATCGACGAGGAGCACGACGGTTCCTATAAACAGCAGGAGGGCCTGCGCTACCACGCCCGGGACCTGGCCCTGGT
CCGCGCCCGCCAGGAAGACATCCCGATCGTCCTGGGTTCGGCCACCCCTTCCCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTATGGCCTGCTGCGCATGAACGAACGGGCCGGCGGCGCCAAGCCGCCGCGTTTCCTGCGCCTGGACGTCAAGAGC
CGGCCGCTGGACAGCGGCATCTCCGGCCCCATGCAGCAGGCTATCGGCCAGACCCTGGCCGCCGGGCAACAGGTCCTGGT
GTTCCTCAATCGCCGCGGCTTCGCCCCGACCCTGCTGTGCCATGACTGCGGCTGGATGTCCGAATGCGAGCGCTGCGACG
CCCGCATGACGGTACACCAACGCTCGGGCGAGCTGCGTTGCCACCATTGCGGCCATGTGGAGCGAGTGCCGCGGCATTGC
CCGCAGTGCGGCAAGGTCGATCTGCGGCCGGTGGGCGCCGGCACCGAACGGGCGGAAGAACGCCTGGGCATCCTGTTCCC
CGACTACCCGGTCCTGCGGGTGGACCGCGACAGCACCTCGCGCAAGGACGCGATGAACCAGCTGTTCGCCACCATCCAGA
AGGGCCAGCCGTGCATCCTGGTGGGCACGCAGATGCTCGCCAAGGGGCATCACTTCCCCAGGGTGACCCTGGTGGCGATC
CTGGATGCCGACGGCGGCCTGTTTTCCGGGGACTTTCGCGCCAGCGAACGCATGGCGCAATTGATCGTCCAGGTGGCCGG
CCGCGCAGGCCGCGCCGAGGAACCGGGCAAGGTGATCATCCAGACGCACTTGGCCGATCACCCGCTGCTGGTGCAGCTGA
CCGAGCAAGGCTACTTCGCCTTCGCCGAGCAAGCCCTGAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACTTG
GCGCTGCTACGGGCCGAAGCCCACAAGCCGGGCCAGGCCGAGGCCTTCCTCGACGAAGCTTGCAGCGAGGCCGAGCGCCT
GCTGGCCGAACAGCAGTCGGCAGGGATCGAATTGCTCGGACCGGTGCCCGCGCCCATGGAGCGCCGGGCCGGACGCTATC
GAGCGCAATTGCTGCTGCAGGCCAATGCCCGGGCACCGCTGCACCGCCTGCTCAGCAGCTGGCTGCTGGTGCTGGAGCAG
ATGCCCGGCGGCCGGCAGGTTCGCTGGTCACTGGATGTGGACCCGGTCGACCTGTACTGA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Helicase conserved C-terminal domain (Helicase_C)

(522-592)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(207-367)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X1PQF3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

48.188

100

0.486

Multiple sequence alignment