Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AM379_RS05245 Genome accession   NZ_CP027618
Coordinates   1079979..1081412 (+) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter cloacae complex sp. FDA-CDC-AR_0132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1074979..1086412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM379_RS05215 (AM379_05210) - 1075546..1076523 (+) 978 WP_020883528.1 calcium/sodium antiporter -
  AM379_RS05220 (AM379_05215) kdsD 1076537..1077523 (+) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  AM379_RS05225 (AM379_05220) kdsC 1077538..1078104 (+) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AM379_RS05230 (AM379_05225) lptC 1078101..1078676 (+) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  AM379_RS05235 (AM379_05230) lptA 1078645..1079199 (+) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AM379_RS05240 (AM379_05235) lptB 1079206..1079931 (+) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  AM379_RS05245 (AM379_05240) rpoN/rpoN1 1079979..1081412 (+) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  AM379_RS05250 (AM379_05245) hpf 1081435..1081722 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  AM379_RS05255 (AM379_05250) ptsN 1081805..1082296 (+) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AM379_RS05260 (AM379_05255) rapZ 1082342..1083196 (+) 855 WP_014171820.1 RNase adapter RapZ -
  AM379_RS05265 (AM379_05260) npr 1083193..1083465 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  AM379_RS05270 (AM379_05265) mtgA 1083504..1084229 (-) 726 WP_032679051.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AM379_RS05275 (AM379_05270) elbB 1084226..1084879 (-) 654 WP_032679052.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=278518 AM379_RS05245 WP_014171817.1 1079979..1081412(+) (rpoN/rpoN1) [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=278518 AM379_RS05245 WP_014171817.1 1079979..1081412(+) (rpoN/rpoN1) [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGTAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATAGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATCCAACTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTAATCATCAGCGACCATCTGGATCTTCTGGCCAATCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486

Multiple sequence alignment