Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   AM480_RS04540 Genome accession   NZ_CP027611
Coordinates   911351..911776 (-) Length   141 a.a.
NCBI ID   WP_000788342.1    Uniprot ID   N9K1U0
Organism   Acinetobacter baumannii strain AR_0101     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 906351..916776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM480_RS04510 (AM480_04510) - 906880..907914 (+) 1035 WP_001189451.1 lipase secretion chaperone -
  AM480_RS04515 (AM480_04515) - 908029..908970 (+) 942 WP_085943440.1 lipase family alpha/beta hydrolase -
  AM480_RS04520 (AM480_04520) rplS 909023..909391 (-) 369 WP_000014562.1 50S ribosomal protein L19 -
  AM480_RS04525 (AM480_04525) trmD 909599..910339 (-) 741 WP_000464596.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  AM480_RS04530 (AM480_04530) rimM 910385..910933 (-) 549 WP_000189236.1 ribosome maturation factor RimM -
  AM480_RS04535 (AM480_04535) rpsP 910953..911204 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  AM480_RS04540 (AM480_04540) pilE 911351..911776 (-) 426 WP_000788342.1 type IV pilin protein Machinery gene
  AM480_RS04545 (AM480_04545) pilY2 911773..912255 (-) 483 WP_001046417.1 type IV pilin protein Machinery gene
  AM480_RS04550 (AM480_04550) pilY1 912268..916122 (-) 3855 WP_213065396.1 PilC/PilY family type IV pilus protein Machinery gene

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15214.45 Da        Isoelectric Point: 7.8464

>NTDB_id=278469 AM480_RS04540 WP_000788342.1 911351..911776(-) (pilE) [Acinetobacter baumannii strain AR_0101]
MKNGFTLIEIMIVVAIIAILAAIATPSYLQYLRKGHRTAVQSEMMNIAQTLESEKVVHNRYPSNATIQSIYGSNVSPIQG
QALYKLAFATLNDSTWVLTATPISTSSQAGDGIICLNDQGQKFWAKGATVCALSASSSWTE

Nucleotide


Download         Length: 426 bp        

>NTDB_id=278469 AM480_RS04540 WP_000788342.1 911351..911776(-) (pilE) [Acinetobacter baumannii strain AR_0101]
ATGAAGAATGGTTTTACTTTAATAGAAATCATGATTGTAGTAGCGATAATTGCAATTTTAGCAGCTATAGCGACACCTTC
ATATTTGCAATATTTACGTAAAGGGCATCGTACAGCTGTTCAATCCGAAATGATGAATATTGCCCAAACATTAGAATCAG
AAAAAGTAGTTCACAATCGTTATCCGTCGAATGCGACCATACAGTCGATTTATGGTTCTAATGTGAGTCCTATACAAGGT
CAGGCTCTATATAAGTTAGCTTTTGCAACTCTGAATGATTCAACTTGGGTACTTACTGCAACACCTATTTCTACTAGCTC
ACAAGCAGGTGATGGAATTATTTGTTTAAATGACCAAGGGCAAAAATTTTGGGCAAAAGGTGCCACAGTTTGTGCACTAT
CTGCTTCATCAAGTTGGACAGAGTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(26-115)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-24)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9K1U0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

92.199

100

0.922
  comF Acinetobacter baylyi ADP1

52.143

99.291

0.518

Multiple sequence alignment