Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   D8Z71_RS00940 Genome accession   NZ_CP032801
Coordinates   178965..179633 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain ERL06-2442     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 173965..184633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8Z71_RS00910 (D8Z71_00930) yicC 173984..174847 (-) 864 WP_000621337.1 YicC/YloC family endoribonuclease -
  D8Z71_RS00915 (D8Z71_00935) rph 174974..175690 (+) 717 WP_001247093.1 ribonuclease PH -
  D8Z71_RS00920 (D8Z71_00940) pyrE 175756..176397 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  D8Z71_RS00925 (D8Z71_00945) slmA 176434..177030 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  D8Z71_RS00930 (D8Z71_00950) dut 177137..177595 (-) 459 WP_000976070.1 dUTP diphosphatase -
  D8Z71_RS00935 (D8Z71_00955) coaBC 177573..178793 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  D8Z71_RS00940 (D8Z71_00960) radC 178965..179633 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  D8Z71_RS00945 (D8Z71_00965) rpmB 179850..180086 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  D8Z71_RS00950 (D8Z71_00970) rpmG 180107..180274 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  D8Z71_RS00955 (D8Z71_00975) mutM 180372..181181 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  D8Z71_RS00960 (D8Z71_00980) coaD 181220..181699 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  D8Z71_RS00965 (D8Z71_00985) waaA 181707..182984 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  D8Z71_RS00970 (D8Z71_00990) rfaQ 183397..184455 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=278345 D8Z71_RS00940 WP_001297375.1 178965..179633(+) (radC) [Escherichia coli strain ERL06-2442]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=278345 D8Z71_RS00940 WP_001297375.1 178965..179633(+) (radC) [Escherichia coli strain ERL06-2442]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496