Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C6362_RS08565 Genome accession   NZ_CP027570
Coordinates   1788334..1789278 (+) Length   314 a.a.
NCBI ID   WP_014017056.1    Uniprot ID   G0VLY9
Organism   Megasphaera elsdenii DSM 20460 strain ATCC 25940     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1783334..1794278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6362_RS08555 (C6362_08555) - 1783577..1784530 (-) 954 WP_014017058.1 magnesium transporter CorA family protein -
  C6362_RS08560 (C6362_08560) smc 1784777..1788331 (+) 3555 WP_014017057.1 chromosome segregation protein SMC -
  C6362_RS08565 (C6362_08565) pilA 1788334..1789278 (+) 945 WP_014017056.1 signal recognition particle-docking protein FtsY Machinery gene
  C6362_RS08570 (C6362_08570) - 1789290..1789754 (+) 465 WP_014017055.1 YhcH/YjgK/YiaL family protein -
  C6362_RS08575 (C6362_08575) thiM 1789769..1790581 (+) 813 WP_014017054.1 hydroxyethylthiazole kinase -
  C6362_RS08580 (C6362_08580) thiW 1790574..1791077 (+) 504 WP_014017053.1 energy coupling factor transporter S component ThiW -
  C6362_RS08585 (C6362_08585) - 1791211..1791801 (+) 591 WP_014017052.1 TetR/AcrR family transcriptional regulator -
  C6362_RS08590 (C6362_08590) - 1791804..1792976 (+) 1173 WP_014017051.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 33780.64 Da        Isoelectric Point: 4.7563

>NTDB_id=278212 C6362_RS08565 WP_014017056.1 1788334..1789278(+) (pilA) [Megasphaera elsdenii DSM 20460 strain ATCC 25940]
MGFFSKLRKGLEKTKHSLIQNIETVVRGYAKIDDDMYDDLEAVMLTGDIGVETTEYLLDQIRTGVKSKEIKDGNDVVPYL
EKCIVALLNENDEPLPETEGPRVIFIVGVNGVGKTTTIGKLAKYYKQQGKSVMLAAGDTFRAAASEQLTIWADRVGVPIV
KHQEGADAAAVVFDATASAKAKGMDILLVDTAGRLQTKSNLMEELRKMARVAGRNIEGAPQETLLVLDATTGQNAVSQAK
LFGDVVPLTGVVLTKLDGTAKGGVILSVKRELGVPVRWVGVGEGVDDLRPFDAAQFADALFDKNVAAREASDED

Nucleotide


Download         Length: 945 bp        

>NTDB_id=278212 C6362_RS08565 WP_014017056.1 1788334..1789278(+) (pilA) [Megasphaera elsdenii DSM 20460 strain ATCC 25940]
ATGGGATTTTTTTCAAAACTTCGAAAAGGCTTAGAAAAGACGAAACACTCGCTCATCCAGAATATCGAAACCGTCGTCCG
CGGCTATGCCAAAATAGACGACGACATGTACGATGACCTGGAAGCGGTCATGCTCACCGGCGACATCGGCGTAGAAACGA
CGGAATACCTCCTGGACCAGATCCGGACCGGCGTCAAATCGAAGGAAATCAAAGACGGCAACGACGTCGTACCGTATTTG
GAAAAGTGCATCGTCGCCTTGCTCAATGAAAATGATGAACCCCTCCCGGAAACGGAAGGGCCGCGGGTCATCTTCATCGT
CGGCGTCAATGGTGTCGGTAAGACGACGACTATCGGCAAACTGGCCAAGTATTACAAACAGCAGGGGAAATCGGTCATGC
TCGCCGCCGGCGATACCTTCCGTGCCGCTGCCTCAGAACAGCTGACCATCTGGGCTGACCGCGTCGGCGTACCCATCGTC
AAGCACCAGGAAGGGGCGGACGCAGCGGCTGTCGTCTTCGATGCGACGGCGTCGGCCAAGGCCAAGGGCATGGATATCCT
CCTTGTCGATACGGCCGGCCGCCTGCAGACCAAGTCCAACCTCATGGAAGAATTGCGGAAAATGGCCCGCGTCGCCGGCC
GCAACATCGAAGGGGCGCCGCAGGAAACGCTTCTCGTCCTCGATGCCACGACGGGGCAGAACGCTGTCAGCCAGGCCAAG
CTCTTCGGCGACGTCGTTCCCTTGACGGGCGTCGTCCTGACCAAGCTCGACGGAACAGCCAAAGGCGGCGTCATCTTGTC
GGTGAAACGGGAATTAGGCGTCCCTGTCCGCTGGGTCGGCGTCGGCGAAGGCGTCGACGACCTGCGCCCCTTCGATGCAG
CTCAGTTCGCCGATGCTCTGTTCGATAAAAACGTCGCCGCCCGCGAAGCCAGCGACGAAGATTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(21-80)

SRP54-type protein, GTPase domain (SRP54)

(101-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0VLY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.645

96.815

0.5

Multiple sequence alignment