Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C6362_RS00815 Genome accession   NZ_CP027570
Coordinates   169228..170607 (-) Length   459 a.a.
NCBI ID   WP_014016306.1    Uniprot ID   G0VQ48
Organism   Megasphaera elsdenii DSM 20460 strain ATCC 25940     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 164228..175607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6362_RS00785 (C6362_00785) - 164791..165174 (-) 384 WP_014016312.1 helix-turn-helix domain-containing protein -
  C6362_RS00790 (C6362_00790) - 165307..166380 (+) 1074 WP_014016311.1 NADH:flavin oxidoreductase -
  C6362_RS00795 (C6362_00795) - 166428..166664 (+) 237 WP_106699278.1 alpha/beta hydrolase -
  C6362_RS00800 (C6362_00800) - 166725..167930 (-) 1206 WP_014016309.1 MalY/PatB family protein -
  C6362_RS00805 (C6362_00805) - 168046..168342 (-) 297 WP_014016308.1 hypothetical protein -
  C6362_RS00810 (C6362_00810) - 168353..168682 (-) 330 WP_014016307.1 hypothetical protein -
  C6362_RS00815 (C6362_00815) radA 169228..170607 (-) 1380 WP_014016306.1 DNA repair protein RadA Machinery gene
  C6362_RS00820 (C6362_00820) - 170617..173046 (-) 2430 WP_173364628.1 ATP-dependent Clp protease ATP-binding subunit -
  C6362_RS00825 (C6362_00825) - 173057..174121 (-) 1065 WP_014016304.1 ATP--guanido phosphotransferase -
  C6362_RS00830 (C6362_00830) - 174125..174640 (-) 516 WP_014016303.1 UvrB/UvrC motif-containing protein -
  C6362_RS00835 (C6362_00835) - 174664..175119 (-) 456 WP_014016302.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49471.04 Da        Isoelectric Point: 7.2263

>NTDB_id=278205 C6362_RS00815 WP_014016306.1 169228..170607(-) (radA) [Megasphaera elsdenii DSM 20460 strain ATCC 25940]
MAKRKTRYVCTNCGSVSSRWLGRCPQCGEWNTMTEETVEPEAPKAAQSMARSGPASQAASLADIAMEDMARMETGIGELD
RVLGGGIVPGALILLSGDPGIGKSTLVLQLAAAVCEKNGSVLYGSGEESAGQIKMRAQRLGISTPQLIIQADTSLDNILD
EAKRRRPALLVVDSIQTMYSRDIEGTPGSLAQIREGTSRLMTFAKSEGIPVVVIGHVTKDGAIAGPRMMEHMVDVVLYFE
GERNYQFRILRGIKNRFGSTNESGLFTMTSGGLSELENPSQLLLAERAADQAGSAVAAVLDGMRPMLGEIQALTAHSVFA
VPRRTASGMDYNRLIILLAVLEKRLGVPLGSQDVYINVVGGLRLTETAADLPVALAVYSSLRDISMDSRTVVMGEVGLTG
DIRRVPQALRRVKEAAKMGFNTFILPKGNLEDIEKDKFPDCHFLGVATLREAIEKAFRR

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=278205 C6362_RS00815 WP_014016306.1 169228..170607(-) (radA) [Megasphaera elsdenii DSM 20460 strain ATCC 25940]
ATGGCAAAGAGAAAGACCCGTTACGTCTGCACCAACTGTGGCAGCGTGTCCAGCCGCTGGCTCGGCCGCTGCCCGCAGTG
CGGTGAATGGAATACCATGACAGAAGAGACGGTCGAGCCGGAAGCGCCTAAGGCTGCCCAGAGCATGGCCCGCAGCGGCC
CGGCGTCCCAGGCGGCATCCTTGGCGGATATCGCCATGGAAGACATGGCCCGCATGGAAACGGGCATCGGCGAACTGGAC
CGCGTCCTCGGCGGCGGCATCGTCCCGGGCGCCCTGATCCTCCTCAGCGGCGACCCGGGCATCGGCAAGTCGACACTGGT
CCTGCAGCTGGCGGCGGCAGTCTGCGAAAAGAACGGCAGCGTCCTCTACGGTTCCGGTGAAGAATCGGCAGGCCAGATCA
AGATGCGTGCCCAGCGCCTGGGCATTTCCACGCCGCAGCTGATTATCCAGGCCGACACGTCCCTGGACAACATCCTCGAC
GAAGCGAAGCGGCGCCGCCCGGCCCTGCTCGTCGTCGACTCCATCCAGACCATGTACAGCCGCGACATCGAAGGCACGCC
GGGCAGCCTGGCCCAGATTCGCGAAGGCACGAGCCGCCTCATGACCTTTGCCAAGAGCGAAGGCATCCCGGTCGTCGTCA
TCGGCCACGTCACCAAGGACGGCGCCATCGCCGGGCCGCGCATGATGGAGCACATGGTCGATGTCGTCCTCTACTTCGAA
GGCGAACGGAACTACCAGTTCCGCATCCTGCGGGGCATCAAGAACCGCTTTGGCTCGACGAACGAATCGGGTCTCTTCAC
CATGACTAGCGGCGGCCTTTCGGAACTGGAAAATCCGTCGCAGCTACTCTTGGCCGAACGGGCAGCGGACCAGGCAGGCT
CTGCCGTGGCCGCTGTCCTCGACGGGATGCGGCCCATGCTCGGTGAAATCCAGGCCCTGACGGCTCATTCCGTCTTCGCC
GTACCGCGCCGCACGGCGTCGGGCATGGACTATAACCGCCTGATCATCCTCTTGGCGGTCCTGGAAAAACGCCTGGGTGT
TCCCTTGGGGAGTCAGGACGTCTATATCAACGTCGTCGGCGGCCTGCGCCTGACCGAAACGGCAGCGGACCTGCCCGTAG
CCCTGGCCGTCTACTCCAGTCTGCGGGACATCTCCATGGACAGCCGCACTGTCGTCATGGGCGAAGTCGGCCTCACCGGC
GACATCCGCCGCGTCCCCCAGGCCCTGCGCCGCGTCAAAGAAGCTGCTAAGATGGGCTTCAACACCTTCATCCTGCCCAA
AGGCAACCTGGAAGACATTGAAAAAGATAAATTCCCGGACTGCCATTTCCTGGGCGTAGCTACCCTGCGCGAAGCCATCG
AAAAGGCCTTTCGGAGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(69-221)

Subunit ChlI of Mg-chelatase (ChlI)

(346-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0VQ48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.908

99.782

0.488
  radA Streptococcus mitis NCTC 12261

47.484

99.564

0.473
  radA Streptococcus pneumoniae Rx1

47.484

99.564

0.473
  radA Streptococcus pneumoniae D39

47.484

99.564

0.473
  radA Streptococcus pneumoniae R6

47.484

99.564

0.473
  radA Streptococcus pneumoniae TIGR4

47.484

99.564

0.473
  radA Streptococcus mitis SK321

47.265

99.564

0.471

Multiple sequence alignment