Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C6Y28_RS08820 Genome accession   NZ_CP027569
Coordinates   1829932..1830876 (+) Length   314 a.a.
NCBI ID   WP_027895712.1    Uniprot ID   A0A1M6MYQ3
Organism   Megasphaera elsdenii strain NCIMB702410     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1824932..1835876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y28_RS08810 (C6Y28_08810) - 1825175..1826128 (-) 954 WP_014017058.1 magnesium transporter CorA family protein -
  C6Y28_RS08815 (C6Y28_08815) smc 1826375..1829929 (+) 3555 WP_027895711.1 chromosome segregation protein SMC -
  C6Y28_RS08820 (C6Y28_08820) pilA 1829932..1830876 (+) 945 WP_027895712.1 signal recognition particle-docking protein FtsY Machinery gene
  C6Y28_RS08825 (C6Y28_08825) - 1830888..1831352 (+) 465 WP_014017055.1 YhcH/YjgK/YiaL family protein -
  C6Y28_RS08830 (C6Y28_08830) thiM 1831367..1832179 (+) 813 WP_014017054.1 hydroxyethylthiazole kinase -
  C6Y28_RS08835 (C6Y28_08835) thiW 1832172..1832675 (+) 504 WP_027895713.1 energy coupling factor transporter S component ThiW -
  C6Y28_RS08840 (C6Y28_08840) - 1832809..1833399 (+) 591 WP_027895714.1 TetR/AcrR family transcriptional regulator -
  C6Y28_RS08845 (C6Y28_08845) - 1833402..1834574 (+) 1173 WP_014017051.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 33766.61 Da        Isoelectric Point: 4.7563

>NTDB_id=278201 C6Y28_RS08820 WP_027895712.1 1829932..1830876(+) (pilA) [Megasphaera elsdenii strain NCIMB702410]
MGFFSKLRKGLEKTKHSLIQNIESVVRGYAKIDDDMYDDLEAVMLTGDIGVETTEYLLDQIRTGVKSKEIKDGNDVVPYL
EKCIVALLNENDEPLPETEGPRVIFIVGVNGVGKTTTIGKLAKYYKQQGKSVMLAAGDTFRAAASEQLTIWADRVGVPIV
KHQEGADAAAVVFDATASAKAKGMDILLVDTAGRLQTKSNLMEELRKMARVAGRNIEGAPQETLLVLDATTGQNAVSQAK
LFGDVVPLTGVVLTKLDGTAKGGVILSVKRELGVPVRWVGVGEGVDDLRPFDAAQFADALFDKNVAAREASDED

Nucleotide


Download         Length: 945 bp        

>NTDB_id=278201 C6Y28_RS08820 WP_027895712.1 1829932..1830876(+) (pilA) [Megasphaera elsdenii strain NCIMB702410]
ATGGGATTTTTTTCAAAACTTCGAAAAGGCTTAGAAAAGACGAAACATTCGCTCATCCAGAACATCGAGTCCGTCGTCCG
CGGTTATGCCAAGATCGACGACGACATGTACGATGACCTGGAAGCGGTCATGCTCACCGGCGACATCGGCGTCGAAACGA
CGGAATACCTCCTGGACCAGATTCGGACCGGCGTCAAATCGAAGGAAATCAAAGATGGCAACGACGTCGTACCGTATTTG
GAAAAGTGCATCGTCGCCTTGCTCAATGAAAATGATGAACCCCTTCCGGAAACCGAAGGGCCGCGGGTCATCTTCATCGT
CGGCGTCAACGGCGTCGGCAAGACGACGACTATCGGCAAACTGGCCAAGTATTACAAGCAGCAGGGGAAATCGGTCATGC
TCGCCGCCGGCGATACCTTCCGCGCCGCTGCCTCGGAACAGCTGACCATCTGGGCTGACCGCGTCGGCGTACCCATCGTC
AAGCACCAGGAAGGGGCGGACGCAGCGGCTGTCGTCTTTGATGCGACGGCGTCGGCCAAGGCCAAGGGCATGGATATCCT
CCTCGTCGATACGGCCGGCCGCCTGCAGACCAAGTCCAACCTCATGGAAGAATTGCGGAAAATGGCCCGCGTCGCCGGCC
GCAACATCGAAGGGGCGCCGCAGGAAACGCTCCTCGTCCTCGATGCCACGACGGGGCAGAACGCTGTCAGCCAGGCCAAG
CTCTTCGGCGACGTCGTTCCCTTGACGGGCGTCGTCCTGACCAAGCTCGACGGAACAGCCAAAGGCGGCGTCATCTTGTC
GGTGAAACGGGAATTAGGCGTCCCTGTCCGCTGGGTCGGCGTCGGCGAAGGCGTCGACGACCTGCGCCCCTTCGATGCAG
CTCAGTTCGCCGATGCTCTGTTCGATAAAAACGTCGCCGCCCGCGAAGCCAGCGACGAAGATTAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(101-301)

SRP54-type protein, helical bundle domain (SRP54_N)

(20-80)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1M6MYQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.645

96.815

0.5

Multiple sequence alignment