Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C6Y28_RS00780 Genome accession   NZ_CP027569
Coordinates   177812..179191 (-) Length   459 a.a.
NCBI ID   WP_027894868.1    Uniprot ID   A0A2S0M4A2
Organism   Megasphaera elsdenii strain NCIMB702410     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 172812..184191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y28_RS00755 (C6Y28_00755) - 173737..174135 (-) 399 WP_014016323.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -
  C6Y28_RS00760 (C6Y28_00760) - 174173..175378 (-) 1206 WP_027894865.1 MalY/PatB family protein -
  C6Y28_RS00765 (C6Y28_00765) - 175562..175699 (-) 138 WP_230579032.1 AAA family ATPase -
  C6Y28_RS00770 (C6Y28_00770) - 175880..177142 (-) 1263 WP_027894866.1 AAA family ATPase -
  C6Y28_RS00775 (C6Y28_00775) - 177164..177493 (-) 330 WP_027894867.1 hypothetical protein -
  C6Y28_RS00780 (C6Y28_00780) radA 177812..179191 (-) 1380 WP_027894868.1 DNA repair protein RadA Machinery gene
  C6Y28_RS00785 (C6Y28_00785) - 179201..181630 (-) 2430 WP_027894869.1 ATP-dependent Clp protease ATP-binding subunit -
  C6Y28_RS00790 (C6Y28_00790) - 181641..182705 (-) 1065 WP_027894870.1 ATP--guanido phosphotransferase -
  C6Y28_RS00795 (C6Y28_00795) - 182709..183224 (-) 516 WP_014016303.1 UvrB/UvrC motif-containing protein -
  C6Y28_RS00800 (C6Y28_00800) - 183248..183703 (-) 456 WP_014016302.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49501.06 Da        Isoelectric Point: 7.2263

>NTDB_id=278194 C6Y28_RS00780 WP_027894868.1 177812..179191(-) (radA) [Megasphaera elsdenii strain NCIMB702410]
MAKRKTRYVCTNCGSVSSRWLGRCPQCGEWNTMTEETVEPEAPKAAQSMARSGPASQAASLADIAMEDMARMETGIGELD
RVLGGGIVPGALILLSGDPGIGKSTLVLQLAAAVCEKNGSVLYGSGEESAGQIKMRAQRLGISTPQLIIQADTSLDNILD
EAKRRRPALLVVDSIQTMYSRDIEGTPGSLAQIREGTSRLMTFAKSEGIPVVVIGHVTKDGAIAGPRMMEHMVDVVLYFE
GERNYQFRILRGIKNRFGSTNESGLFTMTSSGLSELENPSQLLLAERAADQAGSAVAAVLDGMRPMLGEIQALTAHSVFA
VPRRTASGMDYNRLIILLAVLEKRLGVPLGSQDVYINVVGGLRLTETAADLPVALAVYSSLRDISMDSRTVVMGEVGLTG
DIRRVPQALRRVKEAAKMGFNTFILPKGNLEDIEKDKFPDCHFLGVATLREAIEKAFRR

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=278194 C6Y28_RS00780 WP_027894868.1 177812..179191(-) (radA) [Megasphaera elsdenii strain NCIMB702410]
ATGGCAAAGAGAAAGACCCGTTACGTCTGCACCAACTGTGGCAGCGTGTCCAGCCGCTGGCTCGGCCGCTGCCCGCAGTG
CGGTGAATGGAATACCATGACAGAAGAGACGGTCGAGCCGGAAGCGCCTAAGGCCGCCCAGAGTATGGCCCGCAGCGGCC
CGGCATCGCAGGCGGCATCCCTGGCGGATATCGCCATGGAAGACATGGCCCGCATGGAAACGGGAATCGGCGAACTGGAC
CGCGTCCTCGGCGGCGGCATCGTCCCAGGAGCGCTGATCCTGCTCAGCGGGGACCCGGGCATCGGCAAGTCGACGCTGGT
CCTGCAGCTGGCGGCGGCAGTCTGCGAAAAGAACGGTAGCGTCCTCTACGGTTCCGGTGAAGAATCGGCAGGGCAGATCA
AGATGCGTGCCCAGCGCCTCGGCATTTCCACGCCGCAGCTGATTATCCAGGCCGACACGTCCCTGGACAACATCCTCGAC
GAAGCGAAGCGGCGCCGCCCGGCCCTGCTCGTCGTCGACTCCATCCAGACCATGTACAGCCGCGACATCGAAGGCACGCC
GGGCAGCCTGGCCCAGATCCGCGAAGGCACGAGCCGCCTCATGACCTTTGCCAAGAGCGAAGGCATTCCGGTCGTCGTCA
TCGGCCACGTCACCAAAGACGGCGCCATCGCCGGGCCGCGCATGATGGAGCACATGGTCGACGTCGTCCTCTACTTCGAA
GGCGAACGGAACTACCAGTTCCGCATCCTGCGGGGCATCAAGAACCGCTTCGGTTCGACCAATGAATCGGGCCTGTTCAC
CATGACCAGCAGCGGGTTGTCGGAACTGGAGAACCCGTCCCAGCTGCTCCTGGCCGAACGGGCAGCCGACCAGGCCGGGT
CAGCCGTGGCCGCCGTCCTCGACGGGATGCGGCCCATGCTCGGCGAAATCCAGGCCCTGACGGCTCATTCCGTCTTCGCC
GTCCCGCGGCGGACGGCATCGGGCATGGACTATAACCGCCTGATCATCCTCCTGGCCGTCTTGGAAAAACGCCTGGGTGT
TCCCTTGGGGAGTCAGGACGTTTACATCAACGTCGTCGGCGGCCTGCGCCTGACCGAAACGGCAGCGGACCTGCCCGTAG
CCCTGGCCGTCTACTCCAGTCTGCGGGACATCTCCATGGACAGCCGCACTGTCGTCATGGGCGAAGTCGGCCTCACCGGC
GACATCCGCCGCGTCCCCCAGGCCCTGCGCCGCGTCAAAGAAGCTGCCAAGATGGGCTTCAACACCTTCATCCTGCCCAA
AGGCAACCTGGAAGACATTGAAAAAGATAAATTCCCGGACTGCCATTTCCTGGGCGTAGCTACCCTGCGCGAAGCCATCG
AAAAGGCCTTTCGGAGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(69-221)

Subunit ChlI of Mg-chelatase (ChlI)

(346-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0M4A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.908

99.782

0.488
  radA Streptococcus mitis NCTC 12261

47.265

99.564

0.471
  radA Streptococcus pneumoniae Rx1

47.265

99.564

0.471
  radA Streptococcus pneumoniae D39

47.265

99.564

0.471
  radA Streptococcus pneumoniae R6

47.265

99.564

0.471
  radA Streptococcus pneumoniae TIGR4

47.265

99.564

0.471
  radA Streptococcus mitis SK321

47.046

99.564

0.468

Multiple sequence alignment