Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AM474_RS20290 Genome accession   NZ_CP027538
Coordinates   4193003..4194364 (+) Length   453 a.a.
NCBI ID   WP_003094867.1    Uniprot ID   A0A0H2ZHH7
Organism   Pseudomonas aeruginosa strain AR_0095     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4188003..4199364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM474_RS20255 (AM474_20255) - 4188100..4188876 (+) 777 WP_019485822.1 ferredoxin--NADP reductase -
  AM474_RS20260 (AM474_20260) mscL 4188981..4189394 (-) 414 WP_003094890.1 large-conductance mechanosensitive channel protein MscL -
  AM474_RS32740 - 4189437..4189925 (+) 489 WP_174490861.1 hypothetical protein -
  AM474_RS20270 (AM474_20270) katB 4189922..4191463 (+) 1542 WP_003094881.1 catalase KatB -
  AM474_RS20275 (AM474_20275) - 4191517..4192068 (+) 552 WP_003141587.1 ankyrin repeat domain-containing protein -
  AM474_RS20280 (AM474_20280) - 4192143..4192397 (+) 255 WP_003094873.1 YdcH family protein -
  AM474_RS20285 (AM474_20285) - 4192459..4192893 (-) 435 WP_003110876.1 CopD family protein -
  AM474_RS20290 (AM474_20290) radA 4193003..4194364 (+) 1362 WP_003094867.1 DNA repair protein RadA Machinery gene
  AM474_RS20295 (AM474_20295) mapZ 4194371..4194748 (-) 378 WP_003094864.1 cyclic di-GMP-binding protein MapZ -
  AM474_RS20300 (AM474_20300) - 4194951..4195457 (+) 507 WP_003094862.1 DUF3015 domain-containing protein -
  AM474_RS20305 (AM474_20305) - 4195795..4197861 (+) 2067 WP_003094859.1 carbon starvation CstA family protein -
  AM474_RS20310 (AM474_20310) - 4197910..4198113 (+) 204 WP_003094856.1 YbdD/YjiX family protein -
  AM474_RS20315 (AM474_20315) yjiA 4198173..4199177 (+) 1005 WP_003094852.1 GTPase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48410.81 Da        Isoelectric Point: 6.8357

>NTDB_id=277863 AM474_RS20290 WP_003094867.1 4193003..4194364(+) (radA) [Pseudomonas aeruginosa strain AR_0095]
MAKAKRMYGCTECGATFPKWAGQCADCGAWNTLVETVVEAAPSGSGRGGWAGQQANLKTLAEVSVEEMPRFTTGSTELDR
VLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLASRVPALYVTGEESQQQVAMRARRLSLPEDKLKVMTETSIETIIATA
RQEQPRVMVIDSIQTIFTEQLQSAPGGVAQVRESAAMLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFEGE
SDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEAVPGSVVMATWEGSRPMLVEVQALVDTSHLANP
RRVTLGLDQNRLAMLLAVLHRHGGIPTYDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSGEV
RPVPSGQERLKEAGKHGFKRAIVPLGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=277863 AM474_RS20290 WP_003094867.1 4193003..4194364(+) (radA) [Pseudomonas aeruginosa strain AR_0095]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGTGGCGCGACCTTCCCGAAATGGGCCGGCCAGTGCGCGGACTG
CGGGGCCTGGAACACCCTGGTGGAGACCGTCGTCGAGGCCGCGCCGAGCGGCTCCGGGCGCGGCGGCTGGGCCGGCCAGC
AGGCCAACCTGAAGACCCTGGCGGAGGTCAGCGTCGAGGAGATGCCGCGCTTCACGACCGGTTCCACCGAACTGGACCGG
GTTCTCGGCGGCGGCCTGGTGGACGGGTCGGTGGTGCTGATCGGCGGCGATCCCGGCATCGGCAAGTCGACCATTCTCCT
GCAGACCCTTTGCAACCTGGCCAGCCGCGTCCCGGCGCTGTACGTCACCGGCGAGGAATCCCAGCAGCAGGTGGCGATGC
GCGCGCGGCGCCTGTCGTTGCCGGAAGACAAGCTCAAGGTGATGACCGAGACCAGCATCGAGACCATCATCGCCACCGCG
CGCCAGGAGCAGCCGCGGGTGATGGTGATCGACTCGATCCAGACCATCTTCACCGAGCAGTTGCAATCGGCGCCGGGCGG
CGTTGCCCAGGTCCGCGAGAGCGCCGCGATGCTGGTGCGCTATGCCAAGCAGAGCGGCACGGCGATCTTCCTGGTCGGCC
ACGTGACCAAGGAAGGCGCCCTGGCCGGCCCGCGGGTGCTCGAACACATGGTCGACACCGTACTGTATTTCGAAGGCGAG
TCAGACGGCCGCCTGCGCCTGCTGCGGGCAGTGAAGAACCGCTTCGGCGCGGTCAACGAACTGGGAGTGTTCGGCATGAC
CGACAAAGGCCTGAAGGAAGTGAGCAACCCTTCGGCGATCTTCCTCACGCGGGCCCAGGAAGCGGTGCCCGGCAGCGTGG
TGATGGCCACCTGGGAAGGCTCGCGGCCGATGCTGGTGGAGGTCCAGGCGCTGGTCGACACCAGTCACCTGGCGAATCCG
CGGCGGGTGACCCTGGGCCTCGACCAGAACCGCCTGGCGATGCTGCTGGCGGTGCTGCACCGGCACGGCGGGATCCCGAC
CTACGACCAGGACGTGTTCCTCAACGTGGTGGGTGGGGTCAAGGTGCTGGAAACCGCCTCCGACCTGGCGTTGATGGCGG
CGGTGATGTCCAGCCTGCGCAACCGCCCGCTGCCGCACGACCTGCTGGTGTTCGGCGAGGTCGGGCTGTCCGGCGAGGTG
CGTCCGGTGCCGAGCGGCCAGGAGCGCCTGAAGGAAGCCGGCAAGCATGGTTTCAAGCGTGCCATCGTACCCCTCGGCAA
CGCGCCGAAGGAGGCGCCGGCGGGATTGCAGGTGATCGCCGTGACGCGCCTCGAGCAGGCCCTGGACGCCCTCTTCGAGT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(74-219)

Subunit ChlI of Mg-chelatase (ChlI)

(361-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZHH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.458

100

0.486
  radA Streptococcus pneumoniae Rx1

46.288

100

0.468
  radA Streptococcus pneumoniae D39

46.288

100

0.468
  radA Streptococcus pneumoniae R6

46.288

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.288

100

0.468
  radA Streptococcus mitis SK321

46.389

100

0.468
  radA Streptococcus mitis NCTC 12261

46.171

100

0.466

Multiple sequence alignment