Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GTNG_RS00525 Genome accession   NC_009328
Coordinates   97410..98783 (+) Length   457 a.a.
NCBI ID   WP_011886621.1    Uniprot ID   A4IJG2
Organism   Geobacillus thermodenitrificans NG80-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92410..103783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTNG_RS00505 (GTNG_0075) ctsR 92783..93244 (+) 462 WP_029761781.1 transcriptional regulator CtsR -
  GTNG_RS00510 (GTNG_0076) - 93259..93807 (+) 549 WP_008882051.1 UvrB/UvrC motif-containing protein -
  GTNG_RS00515 (GTNG_0077) - 93812..94903 (+) 1092 WP_011886620.1 protein arginine kinase -
  GTNG_RS00520 (GTNG_0078) clpC 94900..97335 (+) 2436 WP_008882049.1 ATP-dependent protease ATP-binding subunit ClpC -
  GTNG_RS00525 (GTNG_0079) radA 97410..98783 (+) 1374 WP_011886621.1 DNA repair protein RadA Machinery gene
  GTNG_RS00530 (GTNG_0080) - 98989..100083 (+) 1095 WP_011886622.1 PIN/TRAM domain-containing protein -
  GTNG_RS00535 (GTNG_0081) ispD 100105..100791 (+) 687 WP_008882046.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GTNG_RS00540 (GTNG_0082) ispF 100806..101288 (+) 483 WP_008882045.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GTNG_RS00545 (GTNG_0083) gltX 101498..102967 (+) 1470 WP_011886623.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49413.04 Da        Isoelectric Point: 8.0108

>NTDB_id=27786 GTNG_RS00525 WP_011886621.1 97410..98783(+) (radA) [Geobacillus thermodenitrificans NG80-2]
MAKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIERTKPAVRGAFLHSEPSGPAKPIPITAVTAAQEPRIETKSVEL
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSAQLAAAGHTVLYISGEESVKQVKLRAGRLHAESHQLYVLAEADLEYIV
TAIETIQPACVIVDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTNPADVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRVIVPKNNLTGWQPPAGVNVIGVSHVAEALEHTML

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=27786 GTNG_RS00525 WP_011886621.1 97410..98783(+) (radA) [Geobacillus thermodenitrificans NG80-2]
ATGGCGAAAAAGAAAACAAAATTTGTTTGCCAAGAATGCGGGTATGAATCAGCGAAATGGCTCGGTCGTTGCCCGGGCTG
CCAAACGTGGAACTCGTTCGTTGAAGAAATCGAGCGGACAAAACCGGCTGTGCGCGGGGCGTTTCTTCATTCGGAACCAT
CGGGGCCAGCCAAACCGATTCCGATTACGGCTGTTACGGCAGCACAAGAGCCACGCATTGAAACGAAAAGTGTGGAGCTC
AATCGGGTGCTTGGCGGAGGAATCGTCAAAGGATCGCTCGTCTTAATCGGCGGTGATCCGGGTATTGGCAAATCGACGTT
ATTGCTGCAGACATCGGCACAGCTCGCTGCGGCCGGACATACGGTATTGTATATATCCGGTGAAGAGTCGGTGAAGCAAG
TGAAGCTGCGCGCTGGGCGTCTTCACGCTGAGTCTCATCAACTGTATGTGTTAGCAGAGGCGGATTTGGAATACATTGTA
ACAGCAATTGAAACGATTCAGCCTGCTTGTGTGATTGTCGATTCCATTCAGACGGTGTACCGAACGGATATCACATCGGC
GCCGGGCAGTGTTGCCCAAGTGCGCGAGTGCACCGCTGAGCTAATGAAAATCGCCAAGACGAAAGGCATTGCCATTTTCA
TTGTTGGCCATGTGACCAAAGAAGGGGCGATCGCCGGGCCGCGCCTGCTTGAGCATATGGTTGATACCGTTCTTTATTTT
GAAGGGGAGCGGCATCATACGTACCGCATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCAACGAATGAAATCGGTATTTT
TGAAATGCGTGACATCGGGCTGCGCGAAGTAGAAAATCCGTCGGAAGTGTTTTTGGAAGAACGGTCGCGCGGGGCGGCCG
GCTCGACAGTTGTCGCGGCAATGGAGGGGACGCGTCCGGTGCTTGTTGAAATTCAGGCGCTCGTTTCGCCAACGAGTTTC
GGCAACCCGCGGCGGATGGCGACCGGCCTTGATCATAATCGCGTATCGCTGTTAATGGCTGTGCTCGAAAAGCGGGTCGG
GCTCCTGTTGCAAAACCAGGATGCTTACTTGAAAGTGGCAGGTGGAGTAAAGCTTGATGAACCGGCAATCGATTTGGCGG
TCGCCGTCAGCATTGCCTCGAGCTTCCGCGACCGGCCGACAAATCCAGCCGATGTCATTATCGGCGAAGTCGGCTTGACC
GGGGAAGTGCGCCGCGTTTCCCGCATTGAACAGCGCGTGCAAGAAGCCGTCAAATTAGGCTTTTCACGGGTCATTGTACC
AAAAAACAATTTGACTGGTTGGCAGCCGCCTGCAGGCGTCAATGTGATCGGTGTTTCGCATGTGGCCGAGGCGCTTGAGC
ATACGATGTTATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4IJG2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.836

100

0.748
  radA Streptococcus mitis NCTC 12261

65.121

99.125

0.646
  radA Streptococcus mitis SK321

64.901

99.125

0.643
  radA Streptococcus pneumoniae Rx1

64.68

99.125

0.641
  radA Streptococcus pneumoniae D39

64.68

99.125

0.641
  radA Streptococcus pneumoniae R6

64.68

99.125

0.641
  radA Streptococcus pneumoniae TIGR4

64.68

99.125

0.641

Multiple sequence alignment