Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AM462_RS11940 Genome accession   NZ_CP027528
Coordinates   2436048..2436848 (-) Length   266 a.a.
NCBI ID   WP_005136899.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain AR_0083     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2431048..2441848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM462_RS11920 (AM462_11920) - 2432113..2432811 (-) 699 WP_000058788.1 YfgM family protein -
  AM462_RS11925 (AM462_11925) hisS 2432840..2434132 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  AM462_RS11930 (AM462_11930) ispG 2434129..2435244 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AM462_RS11935 (AM462_11935) - 2435266..2436057 (-) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  AM462_RS11940 (AM462_11940) pilF 2436048..2436848 (-) 801 WP_005136899.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AM462_RS11945 (AM462_11945) rlmN 2436864..2438096 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AM462_RS11950 (AM462_11950) ndk 2438219..2438650 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  AM462_RS11955 (AM462_11955) iscX 2438764..2438964 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  AM462_RS11960 (AM462_11960) - 2439064..2439615 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  AM462_RS11965 (AM462_11965) - 2439640..2440938 (-) 1299 WP_001991191.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30083.42 Da        Isoelectric Point: 9.3928

>NTDB_id=277713 AM462_RS11940 WP_005136899.1 2436048..2436848(-) (pilF) [Acinetobacter baumannii strain AR_0083]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGNI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=277713 AM462_RS11940 WP_005136899.1 2436048..2436848(-) (pilF) [Acinetobacter baumannii strain AR_0083]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCAATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment