Detailed information NTG0027756

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: DRED_RS00990
• Genome accession: NC_009253
• Coordinates: 197727..199076 (+)
• Length: 449 a.a.
• NCBI ID: WP_011876572.1
• Uniprot ID: A4J0X8
• Organism: Desulforamulus reducens MI-1
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	195999..197402	197727..199076	flank	325

Gene organization within MGE regions

Location: 195999..199076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DRED_RS00985 (Dred_0181)	-	195999..197402 (+)	1404	WP_011876571.1	IS1380-like element ISDre4 family transposase	-
DRED_RS00990 (Dred_0182)	radA	197727..199076 (+)	1350	WP_011876572.1	DNA repair protein RadA	Machinery gene

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 48190.54 Da
• Isoelectric Point: 6.6433

>NTDB_id=27756 DRED_RS00990 WP_011876572.1 197727..199076(+) (radA) [Desulforamulus reducens MI-1]
MRNKTTYACQNCGHQTARWMGRCPGCGEWNTLVEERTAVESKKLRGISGTGPVPVTEIIITEEERYATGLVELDRVLGGG
LVPGSLVLVGGDPGIGKSTLLLQAASAIAAGTRKVLYVSGEESARQIKMRAERIGALHPNLYLMAETDMSVVERVIKETT
FSAVVVDSIQTVYQPEISSAPGSVSQVRECTGMLMKLAKGSGVPIFVVGHVTKEGTLAGPRVLEHIVDTVLYFEGDRHQA
YRILRTVKNRFGSTNELGIFEMRGAGLVEVSNPSELFMNQRPENVAGSSIVPSLEGTRPLLVELQALVCASGFGSPRRMT
SGVDYNRVALIMAVLERRVGLRLSSYDAYVSAVGGVKLDEPAADLAIALAIASSFKEQPVQEQVVLVGEVGLTGEVRAVA
GMEKRLQEAAKLGFKVCIGPKDKQSQSMGIIEYIGVQSLQEAVEAAIER

Nucleotide

• Length: 1350 bp

>NTDB_id=27756 DRED_RS00990 WP_011876572.1 197727..199076(+) (radA) [Desulforamulus reducens MI-1]
TTGCGAAATAAAACGACCTATGCCTGCCAGAATTGTGGACATCAGACAGCCAGATGGATGGGAAGGTGCCCCGGTTGTGG
TGAGTGGAATACATTGGTGGAAGAACGGACTGCTGTTGAAAGCAAAAAGCTACGGGGAATTTCGGGTACTGGTCCTGTTC
CTGTAACTGAAATTATTATTACGGAAGAGGAACGATATGCCACAGGTCTTGTAGAGTTGGATCGGGTATTAGGGGGAGGT
TTGGTACCGGGTTCACTGGTTCTTGTAGGAGGGGACCCTGGCATCGGGAAATCTACCCTACTTTTACAGGCAGCCTCTGC
CATTGCAGCGGGTACCCGTAAAGTCCTATACGTCTCTGGAGAAGAATCAGCCCGACAAATCAAGATGAGGGCAGAACGAA
TTGGGGCCCTGCACCCAAATCTGTACTTAATGGCTGAAACGGATATGTCTGTAGTTGAGAGAGTTATAAAGGAAACAACT
TTTTCAGCAGTGGTTGTGGATTCCATTCAAACAGTATATCAGCCAGAAATTTCGTCTGCCCCAGGCAGTGTTTCTCAAGT
AAGGGAATGTACAGGTATGCTGATGAAATTGGCCAAGGGAAGTGGCGTTCCTATTTTTGTCGTGGGGCATGTTACAAAGG
AGGGAACGTTGGCCGGACCCCGGGTGCTGGAGCATATAGTGGATACGGTGCTTTATTTTGAGGGAGATCGGCATCAAGCC
TATCGCATTTTAAGGACAGTGAAAAACCGATTCGGCTCCACCAATGAGCTAGGGATTTTTGAAATGCGGGGGGCAGGATT
AGTAGAAGTATCAAATCCCTCTGAACTATTTATGAATCAACGACCTGAGAATGTGGCGGGGTCCTCAATTGTACCTAGTT
TAGAGGGAACCCGTCCTCTACTAGTTGAGCTCCAGGCACTGGTTTGTGCCAGTGGATTTGGCTCACCCAGGCGTATGACT
TCAGGGGTAGACTACAATCGGGTAGCTTTAATAATGGCGGTTTTAGAACGGCGGGTAGGGCTTCGATTATCTTCTTACGA
TGCCTACGTAAGTGCTGTGGGTGGAGTGAAGTTAGATGAACCAGCTGCCGATTTGGCAATTGCTCTGGCCATAGCTTCAA
GTTTTAAGGAACAACCGGTTCAAGAGCAAGTGGTACTGGTGGGTGAAGTGGGGCTAACCGGCGAAGTCAGGGCTGTAGCA
GGAATGGAGAAGCGGTTACAAGAGGCAGCAAAATTGGGTTTTAAAGTTTGTATAGGACCGAAAGACAAACAAAGCCAATC
CATGGGTATAATAGAATATATAGGAGTACAATCATTGCAAGAGGCAGTGGAAGCCGCCATCGAGCGGTAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (73-215)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (7-34)

• Subunit ChlI of Mg-chelatase (ChlI) (361-422)

3D structure

• AlphaFold DB: A4J0X8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	57.768	100	0.588
radA	Streptococcus pneumoniae Rx1	55.531	100	0.559
radA	Streptococcus pneumoniae D39	55.531	100	0.559
radA	Streptococcus pneumoniae R6	55.531	100	0.559
radA	Streptococcus pneumoniae TIGR4	55.531	100	0.559
radA	Streptococcus mitis NCTC 12261	55.531	100	0.559
radA	Streptococcus mitis SK321	55.531	100	0.559