Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   ECOLC_RS00395 Genome accession   NC_010468
Coordinates   79216..79884 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli ATCC 8739     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 74216..84884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECOLC_RS00365 (EcolC_0067) yicC 74235..75098 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  ECOLC_RS00370 (EcolC_0068) rph 75225..75941 (+) 717 WP_001247089.1 ribonuclease PH -
  ECOLC_RS00375 (EcolC_0069) pyrE 76007..76648 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  ECOLC_RS00380 (EcolC_0070) slmA 76685..77281 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  ECOLC_RS00385 (EcolC_0071) dut 77388..77846 (-) 459 WP_000976070.1 dUTP diphosphatase -
  ECOLC_RS00390 (EcolC_0072) coaBC 77824..79044 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ECOLC_RS00395 (EcolC_0073) radC 79216..79884 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  ECOLC_RS00400 (EcolC_0074) rpmB 80101..80337 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  ECOLC_RS00405 (EcolC_0075) rpmG 80358..80525 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  ECOLC_RS00410 (EcolC_0076) mutM 80623..81432 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ECOLC_RS00415 (EcolC_0077) coaD 81471..81950 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  ECOLC_RS00420 (EcolC_0078) waaA 81958..83235 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  ECOLC_RS00425 (EcolC_0079) rfaQ 83648..84706 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=27745 ECOLC_RS00395 WP_001297375.1 79216..79884(+) (radC) [Escherichia coli ATCC 8739]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=27745 ECOLC_RS00395 WP_001297375.1 79216..79884(+) (radC) [Escherichia coli ATCC 8739]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496