Detailed information NTG0277442

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: D8B36_RS04965
• Genome accession: NZ_CP032667
• Coordinates: 1015824..1016816 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081588.1
• Uniprot ID: Q540P0
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1010824..1021816

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D8B36_RS04935 (D8B36_04935)	ispD	1010885..1011595 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
D8B36_RS04940 (D8B36_04940)	ispF	1011595..1012074 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
D8B36_RS04945 (D8B36_04945)	truD	1012071..1013120 (+)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
D8B36_RS04950 (D8B36_04950)	surE	1013101..1013862 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
D8B36_RS04955 (D8B36_04955)	pcm	1013856..1014482 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
D8B36_RS04960 (D8B36_04960)	nlpD	1014667..1015761 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
D8B36_RS04965 (D8B36_04965)	rpoS	1015824..1016816 (+)	993	WP_000081588.1	RNA polymerase sigma factor RpoS	Regulator
D8B36_RS04970 (D8B36_04970)	ygbN	1016910..1018274 (-)	1365	WP_000104441.1	GntP family transporter	-
D8B36_RS04975 (D8B36_04975)	ygbM	1018363..1019139 (-)	777	WP_001136934.1	HPr family phosphocarrier protein	-
D8B36_RS04980 (D8B36_04980)	ygbL	1019144..1019782 (-)	639	WP_001278994.1	aldolase	-
D8B36_RS04985 (D8B36_04985)	otnK	1019874..1021040 (-)	1167	WP_001393459.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37971.86 Da
• Isoelectric Point: 4.5886

>NTDB_id=277442 D8B36_RS04965 WP_000081588.1 1015824..1016816(+) (rpoS) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=277442 D8B36_RS04965 WP_000081588.1 1015824..1016816(+) (rpoS) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

3D structure

• AlphaFold DB: Q540P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715