Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   D8B36_RS00345 Genome accession   NZ_CP032667
Coordinates   70814..71482 (+) Length   222 a.a.
NCBI ID   WP_001350561.1    Uniprot ID   B1X972
Organism   Escherichia coli str. K-12 substr. MG1655 strain K-12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 65814..76482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8B36_RS00315 (D8B36_00315) yicC 65834..66697 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  D8B36_RS00320 (D8B36_00320) rph 66824..67539 (+) 716 Protein_61 ribonuclease PH -
  D8B36_RS00325 (D8B36_00325) pyrE 67605..68246 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  D8B36_RS00330 (D8B36_00330) slmA 68283..68879 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  D8B36_RS00335 (D8B36_00335) dut 68986..69444 (-) 459 WP_000976073.1 dUTP diphosphatase -
  D8B36_RS00340 (D8B36_00340) coaBC 69422..70642 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  D8B36_RS00345 (D8B36_00345) radC 70814..71482 (+) 669 WP_001350561.1 RadC family protein Machinery gene
  D8B36_RS00350 (D8B36_00350) rpmB 71699..71935 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  D8B36_RS00355 (D8B36_00355) rpmG 71956..72123 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  D8B36_RS00360 (D8B36_00360) mutM 72221..73030 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  D8B36_RS00365 (D8B36_00365) coaD 73069..73548 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  D8B36_RS00370 (D8B36_00370) waaA 73556..74833 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  D8B36_RS00375 (D8B36_00375) rfaQ 75239..76309 (+) 1071 WP_001350560.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25343.46 Da        Isoelectric Point: 8.4958

>NTDB_id=277424 D8B36_RS00345 WP_001350561.1 70814..71482(+) (radC) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHRRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=277424 D8B36_RS00345 WP_001350561.1 70814..71482(+) (radC) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGGCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1X972

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496