Detailed information NTG0277191

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: CFK76_RS04250
• Genome accession: NZ_CP032652
• Coordinates: 842141..845884 (-)
• Length: 1247 a.a.
• NCBI ID: WP_016265072.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain LZ217
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 837141..850884

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFK76_RS04235 (CFK76_04235)	asnS	837278..838576 (-)	1299	WP_011374616.1	asparagine--tRNA ligase	-
CFK76_RS04240 (CFK76_04240)	-	838687..839163 (-)	477	WP_011374615.1	DUF5590 domain-containing protein	-
CFK76_RS04245 (CFK76_04245)	-	839241..842075 (-)	2835	WP_085391439.1	helicase C-terminal domain-containing protein	-
CFK76_RS04250 (CFK76_04250)	addA	842141..845884 (-)	3744	WP_016265072.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
CFK76_RS04255 (CFK76_04255)	-	845868..849428 (-)	3561	WP_016265071.1	PD-(D/E)XK nuclease family protein	-
CFK76_RS04260 (CFK76_04260)	mvk	849705..850652 (+)	948	WP_025015793.1	mevalonate kinase	-

Sequence

Protein

• Length: 1247 a.a.
• Molecular weight: 141571.43 Da
• Isoelectric Point: 4.7686

>NTDB_id=277191 CFK76_RS04250 WP_016265072.1 842141..845884(-) (addA) [Latilactobacillus sakei strain LZ217]
MAILSNPQFTPSQQAAVDHDGHDILVSASAGSGKTSVLVARVIQKILNGTDVDTLLVVTFTEAAATEMRQRIQVALRDAS
EKATEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYNHEYVKE
NEQDTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNNLPKSYAVDEAGLTASDYFQKKILPILQQTITECL
EALQEAEQLSQTSENLMIYAPQIATTQAALTQINADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLDD
VKKKIQTLLDTYFVFDEATTANIQRQAGQLVQKLVSVTLTFRAAFQAEKERRHLLDFSDLEQLCLTILSAEDSPARAFYQ
QKFSEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEAERDSERIILAENFR
SRRNIDDFTNLIFKQLMDENLGELDYDENAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKRI
QAMMTNGEQIWDKKLEKMRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVIDNPNQD
IPLVSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVDL
IWTIYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTIH
GSKGLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTRA
EQRLIIVGHCDNQAQLMKQWQKGQNSTQQILDDGVRNDAASLLDWIGLSLIRHPQAKDWTTDYEPLLALSGDQTEFKLIM
TNETLLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQL
DLAAEQPRQANRYVTDSLAQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQALVDRLVLQNSLSANVAAKINL
NHLLTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSDQ
EAAIERVKTRYAGQINLYSAALNQIAQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3744 bp

>NTDB_id=277191 CFK76_RS04250 WP_016265072.1 842141..845884(-) (addA) [Latilactobacillus sakei strain LZ217]
ATGGCAATCTTGAGTAACCCCCAATTTACACCTTCACAGCAAGCCGCTGTCGATCATGATGGCCACGATATTTTAGTATC
CGCATCAGCTGGTTCAGGGAAGACATCCGTTCTTGTTGCGCGGGTCATCCAAAAAATCCTAAACGGGACCGATGTCGATA
CCTTACTTGTTGTGACGTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGTAGCACTCCGCGACGCCAGT
GAGAAAGCAACAGAACCAGCCGTTAAGCAACGCTTACGGCAACAATTAAGTCTCGTGCCTACCGCACAAATTAGTACACT
GCATGCCTTTTGTTTAAAAGTCATTAAACAATTTTATTATGTCATTGATCGTGATCCCGTATTCCGCTTATTGAGTGATA
CCGCAGAACGACTCCTATTAGCGGATCAAGTATGGCAACGGGTGAGAGAGGCCTTCTATAATCACGAATATGTCAAAGAA
AATGAGCAAGATACATTATTCTACGAATTAGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTGACCGACATTGT
TTTTGAGTTGCTCGATTTTGCAAACGCCAATCCAAACCCGGTTAAATGGTTAAATAACCTTCCCAAAAGTTATGCCGTTG
ACGAAGCTGGTTTGACGGCCAGTGATTATTTCCAAAAAAAAATATTACCGATTTTACAGCAAACCATCACCGAATGTTTG
GAAGCCTTACAAGAAGCTGAACAGCTCAGTCAAACGAGCGAAAATCTAATGATCTATGCCCCTCAAATTGCAACTACGCA
AGCAGCCTTAACACAAATTAACGCAGATGCAAAAACGTTGAATTGGCAAGCATGGCGCCAACAACTAACAGATGCACAGC
TGGGGCCTGCTAAACGGACTAAGAAATTAGAACCCGACGAACAAATTAATAAGGACCGGTTGAAAGCCGATTTAGATGAC
GTCAAAAAGAAAATTCAGACGTTACTGGACACCTATTTTGTGTTTGATGAAGCAACCACCGCTAATATTCAACGTCAAGC
TGGCCAATTGGTTCAAAAATTGGTGAGCGTCACGTTAACTTTTAGAGCAGCCTTTCAAGCCGAAAAAGAACGACGTCATT
TATTGGACTTTAGTGATTTAGAACAACTTTGTCTGACAATTTTATCGGCAGAGGATTCGCCGGCAAGGGCCTTTTACCAA
CAAAAATTTAGCGAAGTGCTAGTTGATGAATATCAAGATACCAATCCGCTCCAAGAAACGATTATCCAAAAGGTCACTTC
TGATCATCCACGCAATCTCTTCATGGTCGGGGATGTTAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCTTAT
TTAAGAATAAGTATAACGAATTTGGTGTCACGGAAGCTGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTCCGT
TCGCGCCGCAATATCGATGACTTTACCAATCTGATTTTTAAACAATTGATGGACGAAAATCTCGGCGAGTTAGACTACGA
CGAAAATGCGGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTTATG
AAACTAAGCCGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGGATT
CAAGCCATGATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATGCGGCCGATTGAATATCGGGACATCGT
TTTACTAGCCCCAACCCGTGGTAATAATTTGTTTATCCTGGATTATTTCAAACGCTTCGGTTTACCGGTTGTGATTAAGG
ATGCGCAAAACTATTTCCAAACGACCGAAGTCCAAATTATGTTAGCTCTACTTCAGGTGATTGATAATCCTAACCAAGAT
ATTCCATTGGTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGATAA
GACGGATGATTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAACGAGCAGAAAGTCGGGCATTTAGGCCAACAGG
TCATGCAGAAGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATTGCTCGCCAACAACCGATTGTGGACTTA
ATTTGGACGATCTACCAAGAAACTGGCTTTTTAGACTATGTCGGTGGGATGCCAGCTGGACGTCAACGTCAAGCTAATTT
ACACGCGCTTTATGAACGTGCCACGGCGTATGAAGAAAATGGCTTTAAAGGACTCTTCCAATTTATTCAATTTATTGAAC
GTCTCCAGAAACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAGGACGCCATCTCTGTTATGACCATTCAC
GGGAGTAAGGGGCTTGAATTTCCAGTCGTCTTTTTAATCGATACGAGTCGTCGCTTTAACCAACAGGATTTACAACGCTC
ATATGTTTTGGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTGCCAACACTGCCAG
AGTTAGCGATTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGTGCG
GAACAACGGTTAATCATCGTTGGCCATTGTGATAACCAGGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAGTAC
CCAACAAATATTAGATGATGGGGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCCATC
CACAAGCTAAAGATTGGACCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAATTTAAGTTGATTATG
ACTAATGAAACCTTACTTGGTGAAATACCAGGTAGTCTGCATAACGATCAGGACTGGGGACAACAACAGCAAAAGGCACT
TCAAACAATTGATTTAAACAGTGGGATAGCAACTGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTGCCA
CCCAAACGACGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGGTTGGGACAATTA
GACCTAGCGGCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGCTTAGCGCAACCACAGTTCTTACAAACCGT
TCAACAACCAACGGCAGCAGAAGTAGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAACGC
TAGCGAGTTTGCAAGCCCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAATCTT
AATCACCTATTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGTGCC
ATTTTCGTTGTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGATCACGTCCTAATCCATGGGA
TTATGGATGGTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCCAGTGATCAA
GAAGCGGCGATTGAACGGGTTAAAACCCGCTATGCTGGCCAGATTAACCTCTATAGTGCTGCTTTAAACCAGATTGCCCA
AAAGCCAGTGACCCATAAATATCTCTATTTATTGTCAATTGGCGAGTTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (10-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1067-1230)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-887)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	37.51	100	0.382