Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   C6I29_RS02135 Genome accession   NZ_CP027365
Coordinates   459431..460234 (+) Length   267 a.a.
NCBI ID   WP_005016505.1    Uniprot ID   A0A2T1J2S1
Organism   Acinetobacter radioresistens strain DSSKY-A-001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454431..465234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6I29_RS02110 - 455358..456635 (+) 1278 WP_034670258.1 glycosyltransferase family 4 protein -
  C6I29_RS02115 - 456664..457215 (+) 552 WP_005023384.1 phosphatase PAP2 family protein -
  C6I29_RS02120 iscX 457303..457503 (+) 201 WP_005016518.1 Fe-S cluster assembly protein IscX -
  C6I29_RS02125 ndk 457617..458048 (+) 432 WP_005016515.1 nucleoside-diphosphate kinase -
  C6I29_RS02130 rlmN 458171..459406 (+) 1236 WP_005023387.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  C6I29_RS02135 pilF 459431..460234 (+) 804 WP_005016505.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  C6I29_RS02140 - 460225..461028 (+) 804 WP_005023389.1 helix-turn-helix domain-containing protein -
  C6I29_RS02145 ispG 461051..462166 (+) 1116 WP_005016500.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  C6I29_RS02150 hisS 462163..463458 (+) 1296 WP_005023391.1 histidine--tRNA ligase -
  C6I29_RS02155 - 463490..464194 (+) 705 WP_034688339.1 YfgM family protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30279.57 Da        Isoelectric Point: 9.5400

>NTDB_id=276601 C6I29_RS02135 WP_005016505.1 459431..460234(+) (pilF) [Acinetobacter radioresistens strain DSSKY-A-001]
MRKPVYKTFVLLTVTLSALLVTACQTPDTLSKDPEKAVKVRTQLAAEYIKSGDLDAAKRALDQALEVDSRDATANMMMGV
LLQQEGSQLNLDKAESYFRRSISIEPKNAQARNNYGAYLYQIGRYKDAIEQLEIAGATLGYEQRYQALENLGRIYLKLGN
VANAEKTFKQALQANRDSSISMLELAEIFYLRQQIPAATQLYEQYVRRVGQKNQGARALWIGIRIARANDDKMGTQVLVN
QLRALFPDSQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=276601 C6I29_RS02135 WP_005016505.1 459431..460234(+) (pilF) [Acinetobacter radioresistens strain DSSKY-A-001]
TTGAGAAAGCCGGTGTATAAAACATTTGTATTACTTACAGTGACTTTGTCGGCCTTGCTTGTTACTGCTTGCCAGACTCC
TGATACCCTCAGTAAGGATCCAGAAAAGGCAGTCAAGGTTCGAACCCAGCTGGCAGCGGAATATATTAAATCGGGTGATC
TGGATGCTGCTAAACGGGCACTAGACCAGGCACTTGAAGTTGATTCCAGAGACGCCACTGCGAACATGATGATGGGAGTA
CTGCTCCAGCAAGAGGGGAGTCAGCTAAATCTGGACAAGGCTGAGAGTTATTTCAGACGCTCTATTTCAATTGAGCCTAA
AAATGCACAGGCGCGTAATAATTATGGCGCCTATTTGTACCAGATCGGTCGTTATAAAGATGCGATCGAACAACTGGAAA
TTGCTGGTGCAACTTTAGGCTATGAGCAGCGTTACCAGGCGCTTGAAAATCTGGGACGAATTTATCTTAAGCTCGGCAAT
GTTGCGAATGCTGAAAAAACATTCAAACAGGCATTGCAAGCTAACCGCGATTCTTCCATTTCAATGTTAGAGTTAGCGGA
AATTTTCTATTTGCGGCAACAGATTCCTGCTGCTACACAGCTTTATGAACAATATGTGCGTCGTGTAGGGCAAAAGAATC
AGGGTGCGCGTGCTCTTTGGATCGGAATTCGAATTGCGCGTGCCAATGATGATAAAATGGGAACACAAGTTTTGGTGAAC
CAGTTACGTGCCTTGTTCCCGGACAGCCAAGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(44-82)

Tetratricopeptide repeat (TPR_16)

(150-208)

Tetratricopeptide repeat (TPR_17)

(98-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T1J2S1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.401

100

0.794
  pilF Acinetobacter baylyi ADP1

73.81

94.382

0.697

Multiple sequence alignment