Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   APL_RS04600 Genome accession   NC_009053
Coordinates   1021263..1022642 (+) Length   459 a.a.
NCBI ID   WP_005601211.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae serovar 5b str. L20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1016263..1027642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APL_RS04570 (APL_0875) yacG 1016542..1016730 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  APL_RS04575 (APL_0876) coaE 1016712..1017350 (-) 639 WP_009874616.1 dephospho-CoA kinase -
  APL_RS04580 (APL_0877) - 1017372..1018010 (-) 639 WP_009874617.1 prepilin peptidase -
  APL_RS04585 (APL_0878) pilC 1018037..1019239 (-) 1203 WP_011848486.1 type II secretion system F family protein Machinery gene
  APL_RS04590 (APL_0879) pilB 1019223..1020617 (-) 1395 WP_005615186.1 GspE/PulE family protein Machinery gene
  APL_RS04595 (APL_0880) pilA 1020635..1021081 (-) 447 WP_009874618.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  APL_RS04600 (APL_0881) radA 1021263..1022642 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  APL_RS04605 (APL_0882) - 1022686..1022919 (-) 234 WP_005597399.1 YgjV family protein -
  APL_RS04610 (APL_0883) folA 1023046..1023534 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  APL_RS04615 (APL_0884) proB 1023621..1024712 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  APL_RS04620 (APL_0885) - 1024725..1025501 (+) 777 WP_005597402.1 TSUP family transporter -
  APL_RS04625 (APL_0886) nth 1025502..1026134 (+) 633 WP_005597404.1 endonuclease III -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49678.24 Da        Isoelectric Point: 7.9094

>NTDB_id=27618 APL_RS04600 WP_005601211.1 1021263..1022642(+) (radA) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKSWNTVSEIRLISSKEATKSDRFSGYAGETSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLSKDLPTLYVTGEESLQQVAMRANRLGLPTDNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLADIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHCIDASI
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSDEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRALPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIIPHGNAPKKPIKGMEVFTVKKLSDALDILGNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=27618 APL_RS04600 WP_005601211.1 1021263..1022642(+) (radA) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGGCTAAAGCACCAAAAACAGCTTATGTATGTAATGATTGCGGCGCGGAATATTCTCGTTGGATGGGGCAATGCAAAGA
ATGTAAGTCTTGGAATACCGTGAGTGAAATTAGACTTATCTCGAGTAAAGAAGCAACAAAAAGCGACCGCTTCAGCGGAT
ATGCCGGCGAAACCTCCGGTAAAGTTCAAACTTTATCCGAAATCAGCTTACAAGAAGTGCCTCGTTTTAGCAGCGGCTTT
AATGAATTGGATCGCGTACTGGGCGGCGGCGTCGTGCCGGGTTCGGCAATTTTAATCGGCGGCCATCCCGGTGCCGGTAA
ATCCACTCTGCTTTTACAAGTAATGTGCGGTTTGTCTAAAGATTTGCCGACCCTTTACGTGACCGGTGAAGAATCCCTAC
AACAAGTGGCGATGCGAGCGAATCGTTTAGGCTTACCGACCGACAATCTCAAGATGCTTTCAGAAACTTCGGTCGAGCAT
ATTTGTAATATCGCCGACCAAGAAAAGCCGAAAATTATGGTAATCGACTCAATTCAGGTCATGCACCTTGCCGATATTCA
ATCTTCTCCCGGTAGCGTTTCGCAAGTGCGGGAATGCGCTTCGTTTCTGACTCGCTATGCAAAAACCCGCCAAGTAGCGA
TTATTATGGTCGGACACGTCACCAAAGACGGGACACTTGCCGGCCCGAAAGTGTTGGAACACTGTATTGACGCCTCAATT
TTACTGGAAGGCGAATCGGACTCTCGATTCCGTACCTTACGCAGCCAGAAAAACCGTTTCGGTGCGGTCAATGAATTGGG
CGTATTTGCCATGACCGAACAAGGCTTACGAGAAGTAAAAAACCCTTCCGCGATTTTCCTCAGCCGTAGCGATGAACAAA
CGCCGGGCAGTTCCGTGATGGTGCTTTGGGAAGGAACCCGTCCGTTATTAGTCGAAATTCAAGCGTTGGTTGATCATTCG
ATGTTGGCTAATCCTCGCCGTGTCGCCGTAGGTTTGGATCACAACCGTTTATCGCTCTTACTTGCGGTGTTACATCGCCA
CGGCGGTTTGCAAATGTCAGATCAAGATGTGTTCGTCAATGTGGTCGGCGGGGTAAAAGTGACCGAGACCAGTGCCGATC
TCGCCCTAATTTTGGCGTTAATTTCCAGTTTTAGAAATCGTGCGTTACCGCAAGATTTGGTCGTATTCGGCGAGGTGGGA
TTAGCCGGAGAAATTCGTCCGGTACCGAGCGGTCAAGAACGTATCAGCGAAGCGGCGAAGCACGGTTTTAAACGTGCGAT
TATTCCTCACGGCAACGCACCTAAAAAACCGATTAAAGGTATGGAAGTCTTTACCGTTAAGAAACTAAGCGACGCACTAG
ATATTCTCGGCAATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.359

99.564

0.481
  radA Streptococcus mitis NCTC 12261

44.493

98.911

0.44
  radA Streptococcus mitis SK321

44.273

98.911

0.438
  radA Streptococcus pneumoniae Rx1

45.581

93.682

0.427
  radA Streptococcus pneumoniae D39

45.581

93.682

0.427
  radA Streptococcus pneumoniae R6

45.581

93.682

0.427
  radA Streptococcus pneumoniae TIGR4

45.581

93.682

0.427

Multiple sequence alignment