Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CSB93_RS32325 Genome accession   NZ_CP027169
Coordinates   6477673..6479166 (+) Length   497 a.a.
NCBI ID   WP_034080943.1    Uniprot ID   A0A2R3IXQ9
Organism   Pseudomonas paraeruginosa strain AR_0356     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6472673..6484166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB93_RS32295 (CSB93_6400) - 6472888..6473697 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  CSB93_RS32300 (CSB93_6402) kdsD 6474107..6475087 (+) 981 WP_170870649.1 arabinose-5-phosphate isomerase KdsD -
  CSB93_RS32305 (CSB93_6403) - 6475087..6475626 (+) 540 WP_012077212.1 KdsC family phosphatase -
  CSB93_RS32310 (CSB93_6404) lptC 6475635..6476207 (+) 573 WP_003155089.1 LPS export ABC transporter periplasmic protein LptC -
  CSB93_RS32315 (CSB93_6405) lptA 6476194..6476721 (+) 528 WP_012077214.1 lipopolysaccharide transport periplasmic protein LptA -
  CSB93_RS32320 (CSB93_6406) lptB 6476721..6477446 (+) 726 WP_003155093.1 LPS export ABC transporter ATP-binding protein -
  CSB93_RS32325 (CSB93_6407) rpoN/rpoN1 6477673..6479166 (+) 1494 WP_034080943.1 RNA polymerase factor sigma-54 Machinery gene
  CSB93_RS32330 (CSB93_6408) hpf 6479244..6479552 (+) 309 WP_003155096.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CSB93_RS32335 (CSB93_6409) ptsN 6479566..6480030 (+) 465 WP_003155098.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CSB93_RS32340 (CSB93_6410) rapZ 6480032..6480892 (+) 861 WP_003155100.1 RNase adapter RapZ -
  CSB93_RS32345 (CSB93_6411) - 6480922..6481194 (+) 273 WP_003155102.1 HPr family phosphocarrier protein -
  CSB93_RS32350 (CSB93_6412) - 6481266..6482168 (-) 903 WP_034080945.1 ZIP family metal transporter -
  CSB93_RS32355 (CSB93_6413) - 6482235..6482849 (-) 615 WP_034080946.1 superoxide dismutase -
  CSB93_RS32360 (CSB93_6414) - 6482862..6483311 (-) 450 WP_012077234.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55940.15 Da        Isoelectric Point: 4.4128

>NTDB_id=275387 CSB93_RS32325 WP_034080943.1 6477673..6479166(+) (rpoN/rpoN1) [Pseudomonas paraeruginosa strain AR_0356]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDGSDPLADGAEQATSAPQESALQE
STTPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSSDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAALDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPPATPWLDEALRLV
SDYLDLLGGRDYGQLMRRMKLKEDELRLVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=275387 CSB93_RS32325 WP_034080943.1 6477673..6479166(+) (rpoN/rpoN1) [Pseudomonas paraeruginosa strain AR_0356]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCTCAGCTGCAACAGGCCATACGCCTTCTCCA
GCTCTCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACGGCAGCGACCCGCTCGCCGATGGCGCCGAACAGGCAACCAGCGCGCCGCAGGAGAGCGCCCTCCAGGAA
AGCACCACGCCCAGCGTAGAAAGCCTCGACGACGACCAGTGGAGCGAGCGCATCCCCAGCGAACTGCCGGTGGATACCGC
CTGGGAGGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAGCGACGACGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTGAACCTGGCGCCGATGTCGGACACCGACCGCATGATCGCG
GTGACCATCATCGACAGCATCAACAATGACGGCTATCTGGAGGAGTCCCTCGAGGAGATCCTCGCCGCCCTCGATCCGGA
GCTGGACGTCGAGCTCGACGAAGTCGAAGTCGTGCTGCGCCGCATCCAGCAGCTGGAGCCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGTTGCTGCAACTGCGCCAGCTGCCACCCGCCACGCCCTGGCTGGACGAAGCCCTGCGCCTGGTT
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACGGCCAGTTGATGCGGCGCATGAAGCTCAAGGAAGACGAGTTGCG
CCTGGTCATCGAGCTGATCCAGTGCCTGCATCCACGGCCCGGCTCGCAGATCGAGTCGAGCGAGGCCGAATACATCGTCC
CGGACGTCATCGTACGCAAGGACAACGAGCGCTGGCTGGTGGAACTGAACCAGGAGGCGATGCCACGCCTGCGTGTCAAC
GCCACCTATGCCGGCATGGTCCGCCGTGCCGACAGCAGTGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCGCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAGACGCTGATGAAGGTGGCGACGCAGATCGTCGAACACCAGCGTGGCT
TCCTCGACTACGGCGAGGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAGTCGACC
ATTTCGCGGGTCACCACGCAGAAGTACATGCATACGCCGCGCGGTATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGCACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTAGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAG
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.785

100

0.481

Multiple sequence alignment