Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CSC02_RS16520 Genome accession   NZ_CP027142
Coordinates   3318728..3320161 (+) Length   477 a.a.
NCBI ID   WP_022649530.1    Uniprot ID   A0AAI9D3Q5
Organism   Enterobacter hormaechei subsp. hoffmannii strain AR_0365     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3313728..3325161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC02_RS16490 (CSC02_3350) - 3314295..3315272 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  CSC02_RS16495 (CSC02_3351) kdsD 3315286..3316272 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  CSC02_RS16500 (CSC02_3352) kdsC 3316287..3316853 (+) 567 WP_017382729.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CSC02_RS16505 (CSC02_3353) lptC 3316850..3317425 (+) 576 WP_022649529.1 LPS export ABC transporter periplasmic protein LptC -
  CSC02_RS16510 (CSC02_3354) lptA 3317394..3317948 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  CSC02_RS16515 (CSC02_3355) lptB 3317955..3318680 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  CSC02_RS16520 (CSC02_3356) rpoN/rpoN1 3318728..3320161 (+) 1434 WP_022649530.1 RNA polymerase factor sigma-54 Machinery gene
  CSC02_RS16525 (CSC02_3357) hpf 3320184..3320471 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  CSC02_RS16530 (CSC02_3358) ptsN 3320554..3321045 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CSC02_RS16535 (CSC02_3359) rapZ 3321091..3321945 (+) 855 WP_003861870.1 RNase adapter RapZ -
  CSC02_RS16540 (CSC02_3360) npr 3321942..3322214 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  CSC02_RS16545 (CSC02_3361) mtgA 3322254..3322979 (-) 726 WP_023298721.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  CSC02_RS16550 (CSC02_3362) elbB 3322976..3323629 (-) 654 WP_023298722.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54128.02 Da        Isoelectric Point: 4.3409

>NTDB_id=275234 CSC02_RS16520 WP_022649530.1 3318728..3320161(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. hoffmannii strain AR_0365]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPE
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISEHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=275234 CSC02_RS16520 WP_022649530.1 3318728..3320161(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. hoffmannii strain AR_0365]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAACAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCTCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAG
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGATTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTTGCTGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGAGCATCTGGATTTGCTGGCAAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTCAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAATCGATCCAGACCAG
CGAACCGGAATACGTTATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAAAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGCCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment