Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   C5695_RS12360 Genome accession   NZ_CP027116
Coordinates   2376871..2377941 (-) Length   356 a.a.
NCBI ID   WP_117730990.1    Uniprot ID   A0AAD0HP33
Organism   Bacillus pumilus strain 145     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2371871..2382941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5695_RS12310 (C5695_12360) - 2371964..2372536 (-) 573 WP_117730981.1 signal peptidase I -
  C5695_RS12315 (C5695_12365) tapA 2372588..2373115 (-) 528 WP_117730982.1 amyloid fiber anchoring/assembly protein TapA -
  C5695_RS12320 (C5695_12370) - 2373415..2373693 (+) 279 WP_233230864.1 DUF3889 domain-containing protein -
  C5695_RS12325 (C5695_12375) - 2373727..2373924 (-) 198 WP_117733119.1 YqzE family protein -
  C5695_RS12330 (C5695_12380) comGG 2373984..2374367 (-) 384 WP_117730984.1 competence type IV pilus minor pilin ComGG -
  C5695_RS12335 (C5695_12385) comGF 2374364..2374891 (-) 528 WP_272866843.1 competence type IV pilus minor pilin ComGF -
  C5695_RS12340 (C5695_12390) comGE 2374803..2375117 (-) 315 WP_117730986.1 competence type IV pilus minor pilin ComGE -
  C5695_RS12345 (C5695_12395) comGD 2375101..2375547 (-) 447 WP_117730987.1 competence type IV pilus minor pilin ComGD -
  C5695_RS12350 (C5695_12400) comGC 2375540..2375833 (-) 294 WP_117730988.1 competence type IV pilus major pilin ComGC Machinery gene
  C5695_RS12355 (C5695_12405) comGB 2375850..2376890 (-) 1041 WP_117730989.1 competence type IV pilus assembly protein ComGB -
  C5695_RS12360 (C5695_12410) comGA 2376871..2377941 (-) 1071 WP_117730990.1 competence type IV pilus ATPase ComGA Machinery gene
  C5695_RS12365 (C5695_12415) - 2378119..2378991 (-) 873 WP_117730991.1 STAS domain-containing protein -
  C5695_RS12375 (C5695_12425) - 2379216..2379596 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  C5695_RS12380 (C5695_12430) - 2379808..2380050 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  C5695_RS12385 (C5695_12435) - 2380099..2380737 (-) 639 WP_117730992.1 MBL fold metallo-hydrolase -
  C5695_RS12390 (C5695_12440) - 2380895..2381068 (+) 174 WP_117730993.1 DUF2759 domain-containing protein -
  C5695_RS12395 (C5695_12445) - 2381143..2381457 (-) 315 WP_117730994.1 MTH1187 family thiamine-binding protein -
  C5695_RS12400 (C5695_12450) - 2381534..2382796 (-) 1263 WP_233230734.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40257.62 Da        Isoelectric Point: 8.8676

>NTDB_id=275061 C5695_RS12360 WP_117730990.1 2376871..2377941(-) (comGA) [Bacillus pumilus strain 145]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEALVSFRVDSDLMQQRIIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVYIRMSTLPTVNDESLVIRILPQNHVPTINHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGEACQLYCKLNRQVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=275061 C5695_RS12360 WP_117730990.1 2376871..2377941(-) (comGA) [Bacillus pumilus strain 145]
TTGTATGGAATTGAATATTTGGGACAAGAACTACTAGAAGAGGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
AAAAGAAAAGGAAGCTTTAGTATCTTTCCGCGTAGATTCTGATTTAATGCAGCAGCGAATCATTGATAAAAAAAGCGGGG
AGCGGCTCATTGCTCATTTTAAATTTTTATCTTCTATGGATATTGGGGAAAAAAGAAGACCACAAAATGGATCACTAGCT
GTGATGTTAAGAAGTGGCCAGGTGTATATTCGAATGTCAACCTTACCGACTGTGAATGATGAGAGCTTAGTCATTAGAAT
TTTACCGCAGAATCATGTTCCGACAATAAACCATCTGTCGTTATTTCCAAAAGCATCCGCTAGGTTATTATCATTTTTGA
ATCATTCGCATGGACTGATTTTATTTACCGGACCAACAAATTCAGGGAAAACAACAACCCTCTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAATCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAGGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATTACATATGCCGCAGGATTAAGAGCAATTTTAAGGCATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCCGAAACGGCCAGAACGGCAATTAGAGCGGCACTGACAGGCCATTTAGTGATGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGCATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACCATGGTTGC
GATTGCAGCGCAGCGATTAATCGAGCTCACCTGTCCATTTTGCGGAGAAGCCTGTCAGCTTTACTGCAAATTAAACCGCC
AGGTCAGACGAACAAATGTATTTGAACTGCTTTTCGGAAAGGAGCTCGGTGAGTGTATCAAAGAGGCCAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGTTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment