Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   C5695_RS08985 Genome accession   NZ_CP027116
Coordinates   1774669..1775103 (+) Length   144 a.a.
NCBI ID   WP_117730427.1    Uniprot ID   A0AAD0HME5
Organism   Bacillus pumilus strain 145     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1769669..1780103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5695_RS08975 (C5695_08985) - 1770229..1773372 (+) 3144 WP_117730425.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  C5695_RS08980 (C5695_08990) - 1773563..1774471 (+) 909 WP_117730426.1 trypsin-like serine protease -
  C5695_RS08985 (C5695_08995) nucA/comI 1774669..1775103 (+) 435 WP_117730427.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  C5695_RS08990 (C5695_09000) - 1775247..1776095 (+) 849 WP_117730428.1 STAS domain-containing protein -
  C5695_RS08995 (C5695_09005) - 1776129..1776671 (-) 543 WP_117730429.1 IseA DL-endopeptidase inhibitor family protein -
  C5695_RS09000 (C5695_09010) - 1776966..1777400 (+) 435 WP_117730430.1 hypothetical protein -
  C5695_RS09005 (C5695_09015) lexA 1777448..1778068 (-) 621 WP_117730431.1 transcriptional repressor LexA -
  C5695_RS09010 (C5695_09020) yneA 1778228..1778539 (+) 312 WP_117730432.1 cell division suppressor protein YneA -
  C5695_RS09015 (C5695_09025) - 1778557..1779204 (+) 648 WP_117730433.1 recombinase family protein -
  C5695_RS09020 (C5695_09030) - 1779274..1779504 (+) 231 WP_117730434.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15982.27 Da        Isoelectric Point: 9.1392

>NTDB_id=275055 C5695_RS08985 WP_117730427.1 1774669..1775103(+) (nucA/comI) [Bacillus pumilus strain 145]
MKMRRGWITAVTLCFLIWIGTLLGGFGEKQAAKGADRYDHIIQFPMERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQ
ESLKGIPTKPGFDRDEWPMAVCLEGGKGASVQYVSPSDNRGAGSWVGHQMSGYPDGKRILFIVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=275055 C5695_RS08985 WP_117730427.1 1774669..1775103(+) (nucA/comI) [Bacillus pumilus strain 145]
TTGAAAATGAGAAGAGGCTGGATCACTGCTGTCACCTTATGCTTTTTGATATGGATAGGCACGCTTTTAGGTGGATTTGG
GGAGAAACAGGCGGCAAAAGGCGCAGATCGATATGATCATATCATTCAATTTCCAATGGAACGATACCCTGAAACAGGCA
GTCATATTCAAGAGGCCATTCGAAAAGGACATTCAGATGTGTGCACGATTGACCGAAATGGAGCAGATGCCCGCAGGCAA
GAATCATTAAAAGGAATTCCAACGAAACCTGGTTTTGACCGGGATGAGTGGCCGATGGCGGTTTGTCTTGAGGGAGGAAA
GGGTGCGAGTGTTCAATATGTCAGCCCATCTGATAATAGGGGCGCAGGCTCATGGGTTGGGCACCAGATGAGCGGTTATC
CTGATGGGAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.824

82.639

0.486

Multiple sequence alignment