Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C5I89_RS20915 Genome accession   NZ_CP027111
Coordinates   4387872..4389305 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain BW     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4382872..4394305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5I89_RS20885 (C5I89_21400) - 4383439..4384416 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  C5I89_RS20890 (C5I89_21405) kdsD 4384430..4385416 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  C5I89_RS20895 (C5I89_21410) kdsC 4385431..4385997 (+) 567 WP_017382729.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  C5I89_RS20900 (C5I89_21415) lptC 4385994..4386569 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  C5I89_RS20905 (C5I89_21420) lptA 4386538..4387092 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  C5I89_RS20910 (C5I89_21425) lptB 4387099..4387824 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  C5I89_RS20915 (C5I89_21430) rpoN/rpoN1 4387872..4389305 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  C5I89_RS20920 (C5I89_21435) hpf 4389328..4389615 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  C5I89_RS20925 (C5I89_21440) ptsN 4389698..4390189 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  C5I89_RS20930 (C5I89_21445) rapZ 4390235..4391089 (+) 855 WP_003861870.1 RNase adapter RapZ -
  C5I89_RS20935 (C5I89_21450) npr 4391086..4391358 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  C5I89_RS20940 (C5I89_21455) mtgA 4391398..4392123 (-) 726 WP_017382728.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  C5I89_RS20945 (C5I89_21460) elbB 4392120..4392773 (-) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=275015 C5I89_RS20915 WP_003861864.1 4387872..4389305(+) (rpoN/rpoN1) [Enterobacter hormaechei strain BW]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=275015 C5I89_RS20915 WP_003861864.1 4387872..4389305(+) (rpoN/rpoN1) [Enterobacter hormaechei strain BW]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTTGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGACGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCATGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment