Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   AM465_RS05100 Genome accession   NZ_CP032201
Coordinates   984518..985186 (+) Length   222 a.a.
NCBI ID   WP_001296533.1    Uniprot ID   B7N279
Organism   Escherichia coli strain AR_0086     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 979518..990186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM465_RS05065 (AM465_05110) yicC 979537..980400 (-) 864 WP_000621332.1 YicC/YloC family endoribonuclease -
  AM465_RS05075 (AM465_05120) rph 980527..981243 (+) 717 WP_001247093.1 ribonuclease PH -
  AM465_RS05080 (AM465_05125) pyrE 981309..981950 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  AM465_RS05085 (AM465_05130) slmA 981987..982583 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  AM465_RS05090 (AM465_05135) dut 982690..983148 (-) 459 WP_000976070.1 dUTP diphosphatase -
  AM465_RS05095 (AM465_05140) coaBC 983126..984346 (-) 1221 WP_000050112.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  AM465_RS05100 (AM465_05145) radC 984518..985186 (+) 669 WP_001296533.1 DNA repair protein RadC Machinery gene
  AM465_RS05105 (AM465_05150) rpmB 985403..985639 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  AM465_RS05110 (AM465_05155) rpmG 985660..985827 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  AM465_RS05115 (AM465_05160) mutM 985925..986734 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  AM465_RS05120 (AM465_05165) coaD 986773..987252 (-) 480 WP_001171854.1 pantetheine-phosphate adenylyltransferase -
  AM465_RS05125 (AM465_05170) waaA 987260..988537 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  AM465_RS05130 (AM465_05175) rfaQ 988951..990009 (+) 1059 WP_000360408.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25286.40 Da        Isoelectric Point: 7.9748

>NTDB_id=274933 AM465_RS05100 WP_001296533.1 984518..985186(+) (radC) [Escherichia coli strain AR_0086]
MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=274933 AM465_RS05100 WP_001296533.1 984518..985186(+) (radC) [Escherichia coli strain AR_0086]
GTGAAAAACAATGTACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(10-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7N279

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment