Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   D2M30_RS08305 Genome accession   NZ_CP032146
Coordinates   1628938..1629300 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain YP6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1623938..1634300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2M30_RS08275 (D2M30_08290) flgD 1624853..1625284 (+) 432 WP_071346811.1 flagellar hook assembly protein FlgD -
  D2M30_RS08280 (D2M30_08295) flgG 1625309..1626085 (+) 777 WP_013352263.1 flagellar basal body rod protein FlgG -
  D2M30_RS08285 (D2M30_08300) - 1626125..1626340 (+) 216 WP_013352264.1 flagellar FlbD family protein -
  D2M30_RS08290 (D2M30_08305) fliL 1626337..1626756 (+) 420 WP_013352265.1 flagellar basal body-associated protein FliL -
  D2M30_RS08295 (D2M30_08310) fliM 1626790..1627788 (+) 999 WP_013352266.1 flagellar motor switch protein FliM -
  D2M30_RS08300 (D2M30_08315) fliY 1627778..1628911 (+) 1134 WP_125122607.1 flagellar motor switch phosphatase FliY -
  D2M30_RS08305 (D2M30_08320) pilH 1628938..1629300 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  D2M30_RS08310 (D2M30_08325) fliZ 1629315..1629968 (+) 654 WP_013352268.1 flagella biosynthesis regulatory protein FliZ -
  D2M30_RS08315 (D2M30_08330) fliP 1629961..1630626 (+) 666 WP_013352269.1 flagellar type III secretion system pore protein FliP -
  D2M30_RS08320 (D2M30_08335) fliQ 1630641..1630910 (+) 270 WP_013352270.1 flagellar biosynthesis protein FliQ -
  D2M30_RS08325 (D2M30_08340) fliR 1630917..1631696 (+) 780 WP_045506167.1 flagellar biosynthetic protein FliR -
  D2M30_RS08330 (D2M30_08345) flhB 1631696..1632775 (+) 1080 WP_088612805.1 flagellar biosynthesis protein FlhB -

Sequence


Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=274823 D2M30_RS08305 WP_003154238.1 1628938..1629300(+) (pilH) [Bacillus amyloliquefaciens strain YP6]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=274823 D2M30_RS08305 WP_003154238.1 1628938..1629300(+) (pilH) [Bacillus amyloliquefaciens strain YP6]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAGGATATTTTAGTGAAGAATGGTTT
TGACGTTGTTGCTGAAGCTTCGGACGGAGCACAGGCAGTCGAAAAATTCAAAGAACATTCACCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACCGCATTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGGCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAGCCATTCCA
AGCAGACCGCGTGCTTGAAGCCATTAATAAAACATTAAGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383


Multiple sequence alignment