Detailed information NTG0274765

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: D2M30_RS01525
• Genome accession: NZ_CP032146
• Coordinates: 270895..272016 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens strain YP6
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 265895..277016

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D2M30_RS01505 (D2M30_01505)	-	266216..267211 (-)	996	WP_125122003.1	DUF5105 domain-containing protein	-
D2M30_RS01510 (D2M30_01510)	-	267329..268726 (+)	1398	WP_125122004.1	YcdB/YcdC domain-containing protein	-
D2M30_RS01515 (D2M30_01515)	-	268927..270285 (+)	1359	WP_125122005.1	YcdB/YcdC domain-containing protein	-
D2M30_RS01520 (D2M30_01520)	-	270282..270785 (-)	504	WP_014469700.1	M15 family metallopeptidase	-
D2M30_RS01525 (D2M30_01525)	rapF	270895..272016 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
D2M30_RS01530 (D2M30_01530)	-	272130..272909 (+)	780	WP_125122006.1	glucose 1-dehydrogenase	-
D2M30_RS01535 (D2M30_01535)	-	272993..274672 (+)	1680	WP_125122007.1	alpha-glucosidase	-
D2M30_RS01540 (D2M30_01540)	-	274867..275826 (+)	960	WP_013350924.1	metal ABC transporter substrate-binding protein	-
D2M30_RS01545 (D2M30_01545)	-	275881..276576 (+)	696	WP_014469701.1	metal ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=274765 D2M30_RS01525 WP_013350919.1 270895..272016(+) (rapF) [Bacillus amyloliquefaciens strain YP6]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=274765 D2M30_RS01525 WP_013350919.1 270895..272016(+) (rapF) [Bacillus amyloliquefaciens strain YP6]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAATCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTACGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAATTCCGTCTGATAAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAACTGTTGGCTGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402