Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   C5Y82_RS05115 Genome accession   NZ_CP027034
Coordinates   1044437..1048141 (+) Length   1234 a.a.
NCBI ID   WP_119124660.1    Uniprot ID   -
Organism   Bacillus pumilus strain 150a     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1039437..1053141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5Y82_RS05100 (C5Y82_05100) - 1039732..1040142 (+) 411 WP_025093362.1 DoxX family protein -
  C5Y82_RS05105 (C5Y82_05105) - 1040339..1040785 (-) 447 WP_041815371.1 ferritin-like domain-containing protein -
  C5Y82_RS05110 (C5Y82_05110) addB 1040950..1044459 (+) 3510 WP_119124659.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  C5Y82_RS05115 (C5Y82_05115) addA 1044437..1048141 (+) 3705 WP_119124660.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  C5Y82_RS05120 (C5Y82_05120) sbcD 1048168..1049343 (+) 1176 WP_012009496.1 exonuclease subunit SbcD -
  C5Y82_RS05125 (C5Y82_05125) sbcC 1049340..1052735 (+) 3396 WP_119124661.1 exonuclease subunit SbcC -
  C5Y82_RS05130 (C5Y82_05130) - 1052755..1053054 (+) 300 WP_012009498.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142384.36 Da        Isoelectric Point: 5.0423

>NTDB_id=274632 C5Y82_RS05115 WP_119124660.1 1044437..1048141(+) (addA) [Bacillus pumilus strain 150a]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYDFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPIVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSSEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYNEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLTAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFSAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALMDGLPVEEYGDADEKMAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLDSLQQRDLLTDEQVQSINQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGIDAAEPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNVEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=274632 C5Y82_RS05115 WP_119124660.1 1044437..1048141(+) (addA) [Bacillus pumilus strain 150a]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
AGTTGCGGCGGCGGCAGGTTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAGATGACCCGGCCTGAACATC
CAGTTGATGTCGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCAGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAATCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTACAGGTTTTACGGACCTTTTACTATGAAATTGATCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGACGAACTGTTTGAGGATGAATATAAGGCTGGGAAGCCATCA
TTCTTTGAATTGGTTGATCGTTACACGTCTGACCGTCATGACTTAGATTTGCAATGGCTCGTGAAGAGAATCTATGATTT
TTCAAGATCTCATCCTTCTCCGGAGCAATGGATGCGGGCATTCCTTTCACTATATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCCTATATCAAAGAAGATCTTTCGCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCCGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTATATGAGCTTTTACCGAACGTCAATTTTAAAAGGCTTAAAACGTGCAAAG
GGGACGAATATGACCCTGTTTTATTAGAGAAAGCTACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAGTACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCGATTGTCGAGACACTTGTAGA
GCTAGTGATCCAATTTGGTGAGCGCTTCGAAAGAGCGAAGCAGGAAAAGTCTATTGTCGATTTTTCCGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACAATTTTAAAACTTGTATCTAAAGGAGA
ACATTCTTCAGAGGGTAATCTGTTTATGGTTGGTGATGTCAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGAAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATAA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACGGGTGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGGATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGGAACTTGCAGTATCGAGACAT
TGTCATTTTACTTCGCTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATCCTTTCTTTGTTAAAAGTCATTGATAATCCATATCAG
GATATTCCGCTTACTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAGTTGGCTCTGATTCGGACGAGTGA
TAAAAAGGGCACGTATTATGATGCTGTAAAGGCCTTTATGAGTGTGACTCATTCTGATCATCCTACATGCAAAAAACTAG
AGCGATTTTTCCAATTACTGCGCAAATGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGCGATACACAGTATCTTGATTATGTTGGGGGAATGCCTGGAGGGAAGCAGCGGCAAGCGAATCTTCGTGCCCTTTATGA
CCGGGCCAAGCAATACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATTCATAGTAGTAAAGGA
TTAGAATTCCCAGTCGTCTTTTCCGCAGGACTTGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAACTAGGATTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAGTTGAGGGTTCTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTCGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCAGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGACCTGCCCTCATACGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGGGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATGGATGGTCTACCTGTTGAAGA
GTATGGTGATGCTGATGAGAAGATGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCCATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAGTCACGTATAGGACACTTGCTCGATTCTTTACAACAGC
GAGACCTGCTAACAGATGAGCAGGTTCAGTCAATTAATCAAGAGGGAATCGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAGGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTAATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTTCAAGGCAGATATACAGGTGGAATTGACGCTGCTGAACCAATCTTGAAAAAGCGTTATGAA
ACCCAAATCGCGTTATATGCAAAAGCGGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTCGA
TGGAAATGTAGAGATATCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

65.939

100

0.66

Multiple sequence alignment