Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C5Y82_RS00590 Genome accession   NZ_CP027034
Coordinates   109374..110753 (+) Length   459 a.a.
NCBI ID   WP_034620767.1    Uniprot ID   A0AAE3WNQ6
Organism   Bacillus pumilus strain 150a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104374..115753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5Y82_RS00570 (C5Y82_00570) - 104716..105180 (+) 465 WP_119124326.1 CtsR family transcriptional regulator -
  C5Y82_RS00575 (C5Y82_00575) - 105195..105752 (+) 558 WP_034665655.1 UvrB/UvrC motif-containing protein -
  C5Y82_RS00580 (C5Y82_00580) - 105757..106848 (+) 1092 WP_003217181.1 protein arginine kinase -
  C5Y82_RS00585 (C5Y82_00585) clpC 106845..109280 (+) 2436 WP_003216969.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  C5Y82_RS00590 (C5Y82_00590) radA 109374..110753 (+) 1380 WP_034620767.1 DNA repair protein RadA Machinery gene
  C5Y82_RS00595 (C5Y82_00595) disA 110756..111835 (+) 1080 WP_012008686.1 DNA integrity scanning diadenylate cyclase DisA -
  C5Y82_RS00600 (C5Y82_00600) - 111989..113089 (+) 1101 WP_012008687.1 PIN/TRAM domain-containing protein -
  C5Y82_RS00605 (C5Y82_00605) ispD 113103..113792 (+) 690 WP_003217158.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  C5Y82_RS00610 (C5Y82_00610) ispF 113796..114272 (+) 477 WP_012008689.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50024.54 Da        Isoelectric Point: 7.9305

>NTDB_id=274622 C5Y82_RS00590 WP_034620767.1 109374..110753(+) (radA) [Bacillus pumilus strain 150a]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSDEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIKSTSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=274622 C5Y82_RS00590 WP_034620767.1 109374..110753(+) (radA) [Bacillus pumilus strain 150a]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGTAAAGAGCCAGCAAACCGTCGAAGCGCTTTTAACCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCAGATGAACCCCGAATCAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGGATTGGGAAGTCCACTTT
ATTATTACAAGTATCAGCACAGCTCTCAGATAAAAATCAGAATGTATTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTCGGCATAAAAAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTCGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
CCCTGGGTCTGTATCTCAAGTGAGAGAATGTACAGCACAGCTGATGAAGATTGCAAAGACAAATGGGATTCCAATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGCCCACGTCTTTTAGAGCATATGGTGGACACGGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGTGCGGTGAAAAACCGATTTGGCTCGACGAATGAACTAGGGATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCCGAAATTTTCTTAGAAGAACGTTCGGCAGGTGTATCTG
GTTCGTGTGTCGTTGCCTCAATGGAAGGAACAAGACCTGTTTTAGTCGAGATACAAGCATTAATTTCACCGACAAGCTTC
GGAAATCCGCGTAGAATGGCCACTGGTCTTGATCATAATCGAGTGTCACTACTCATGGCGGTTTTAGAAAAACGTGTTGG
ACTGCTTCTGCAAAACCAGGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCAGCAATTGACTTGGCCA
TTGCGGTCAGCATTGCATCAAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGCCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCGAAACTAGGATTTAAACGAATGTTTATTCC
TCAGGCAAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment