Detailed information NTG0274422

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: D2195_RS05730
• Genome accession: NZ_CP032078
• Coordinates: 1194724..1195716 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain CT30
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1189724..1200716

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D2195_RS05700 (D2195_06160)	ispD	1189785..1190495 (+)	711	WP_000246133.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
D2195_RS05705 (D2195_06165)	ispF	1190495..1190974 (+)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
D2195_RS05710 (D2195_06170)	truD	1190971..1192020 (+)	1050	WP_212408124.1	tRNA pseudouridine(13) synthase TruD	-
D2195_RS05715 (D2195_06175)	surE	1192001..1192762 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
D2195_RS05720 (D2195_06180)	pcm	1192756..1193382 (+)	627	WP_000254701.1	protein-L-isoaspartate O-methyltransferase	-
D2195_RS05725 (D2195_06185)	nlpD	1193567..1194661 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
D2195_RS05730 (D2195_06190)	rpoS	1194724..1195716 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
D2195_RS05735 (D2195_06195)	-	1195837..1196244 (-)	408	WP_001208064.1	MarR family transcriptional regulator	-
D2195_RS05740 (D2195_06200)	-	1196391..1196984 (+)	594	WP_000767730.1	non-oxidative hydroxyarylic acid decarboxylases subunit B	-
D2195_RS05745 (D2195_06205)	-	1196984..1198411 (+)	1428	WP_000863185.1	non-oxidative hydroxyarylic acid decarboxylases subunit C	-
D2195_RS05750 (D2195_06210)	-	1198422..1198658 (+)	237	WP_000562976.1	non-oxidative hydroxyarylic acid decarboxylases subunit D	-
D2195_RS05755 (D2195_06215)	ygbN	1198696..1200060 (-)	1365	WP_000104432.1	GntP family transporter	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=274422 D2195_RS05730 WP_000081550.1 1194724..1195716(+) (rpoS) [Escherichia coli strain CT30]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=274422 D2195_RS05730 WP_000081550.1 1194724..1195716(+) (rpoS) [Escherichia coli strain CT30]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGCGAGTTGTCCCA
TAAACTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715