Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   D2194_RS00420 Genome accession   NZ_CP032073
Coordinates   81664..82332 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain CT29     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 76664..87332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2194_RS00390 (D2194_00430) yicC 76683..77546 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  D2194_RS00395 (D2194_00440) rph 77673..78389 (+) 717 WP_001247093.1 ribonuclease PH -
  D2194_RS00400 (D2194_00445) pyrE 78455..79096 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  D2194_RS00405 (D2194_00450) slmA 79133..79729 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  D2194_RS00410 (D2194_00455) dut 79836..80294 (-) 459 WP_000976070.1 dUTP diphosphatase -
  D2194_RS00415 (D2194_00460) coaBC 80272..81492 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  D2194_RS00420 (D2194_00465) radC 81664..82332 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  D2194_RS00425 (D2194_00470) rpmB 82549..82785 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  D2194_RS00430 (D2194_00475) rpmG 82806..82973 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  D2194_RS00435 (D2194_00480) mutM 83071..83880 (+) 810 WP_001114529.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  D2194_RS00440 (D2194_00485) coaD 83919..84398 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  D2194_RS00445 (D2194_00490) waaA 84406..85683 (-) 1278 WP_042015822.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  D2194_RS00450 (D2194_00495) rfaQ 86096..87154 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=274322 D2194_RS00420 WP_001297375.1 81664..82332(+) (radC) [Escherichia coli strain CT29]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=274322 D2194_RS00420 WP_001297375.1 81664..82332(+) (radC) [Escherichia coli strain CT29]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment