Detailed information NTG0274272

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: D2J89_RS09210
• Genome accession: NZ_CP032066
• Coordinates: 1926679..1927662 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain E166
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 1921679..1932662

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D2J89_RS09195 (D2J89_09970)	dppA	1922822..1924429 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
D2J89_RS09200 (D2J89_09980)	dppB	1924737..1925756 (+)	1020	WP_000938853.1	dipeptide ABC transporter permease DppB	-
D2J89_RS09205 (D2J89_09985)	dppC	1925766..1926668 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
D2J89_RS09210 (D2J89_09990)	amiE	1926679..1927662 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
D2J89_RS09215 (D2J89_09995)	dppF	1927659..1928663 (+)	1005	WP_000107036.1	dipeptide ABC transporter ATP-binding subunit DppF	-
D2J89_RS09220 (D2J89_10000)	yhjV	1928693..1929964 (-)	1272	WP_001318103.1	aromatic amino acid transport family protein	-
D2J89_RS09225 (D2J89_10010)	-	1930440..1930547 (+)	108	WP_000170738.1	type I toxin-antitoxin system toxin Ldr family protein	-
D2J89_RS09230 (D2J89_10020)	-	1930923..1931030 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=274272 D2J89_RS09210 WP_001196486.1 1926679..1927662(+) (amiE) [Escherichia coli strain E166]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=274272 D2J89_RS09210 WP_001196486.1 1926679..1927662(+) (amiE) [Escherichia coli strain E166]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGGGAGTCTGGCTCCGGTAAATCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTTGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGATAAAGAACGTCT
GGCGTCGTTGCCAGGTGTTGTTCCCGGCAAGTATGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTTGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404