Detailed information NTG0027424

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: MPE_RS16265
• Genome accession: NC_008825
• Coordinates: 3427258..3428310 (-)
• Length: 350 a.a.
• NCBI ID: WP_011830798.1
• Uniprot ID: A2SKT6
• Organism: Methylibium petroleiphilum PM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3422258..3433310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MPE_RS16245 (Mpe_A3218)	-	3423786..3424016 (-)	231	WP_011830794.1	PP0621 family protein	-
MPE_RS16250 (Mpe_A3219)	-	3424062..3424886 (-)	825	WP_011830795.1	inner membrane protein YpjD	-
MPE_RS16255 (Mpe_A3220)	ffh	3424993..3426366 (+)	1374	WP_011830796.1	signal recognition particle protein	-
MPE_RS16260 (Mpe_A3221)	-	3426444..3427241 (+)	798	WP_041929729.1	DUF2726 domain-containing protein	-
MPE_RS16265 (Mpe_A3222)	pilA	3427258..3428310 (-)	1053	WP_011830798.1	signal recognition particle-docking protein FtsY	Machinery gene
MPE_RS16270 (Mpe_A3223)	rsmD	3428441..3429055 (+)	615	WP_011830799.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
MPE_RS16275 (Mpe_A3224)	coaD	3429143..3429592 (+)	450	WP_049820916.1	pantetheine-phosphate adenylyltransferase	-
MPE_RS16280 (Mpe_A3225)	-	3429669..3429968 (+)	300	WP_011830801.1	YfhL family 4Fe-4S dicluster ferredoxin	-
MPE_RS16285 (Mpe_A3226)	-	3429984..3430448 (-)	465	WP_041929730.1	hypothetical protein	-
MPE_RS16290 (Mpe_A3227)	pth	3430457..3431155 (-)	699	WP_011830803.1	aminoacyl-tRNA hydrolase	-
MPE_RS16295 (Mpe_A3228)	-	3431447..3432091 (-)	645	WP_011830804.1	50S ribosomal protein L25/general stress protein Ctc	-
MPE_RS16300 (Mpe_A3229)	-	3432174..3433139 (-)	966	WP_011830805.1	ribose-phosphate pyrophosphokinase	-

Sequence

Protein

• Length: 350 a.a.
• Molecular weight: 36714.41 Da
• Isoelectric Point: 8.8792

>NTDB_id=27424 MPE_RS16265 WP_011830798.1 3427258..3428310(-) (pilA) [Methylibium petroleiphilum PM1]
MFSFFKKKAPVVAPPPTGPEAPAASAPSPGPAAEVRVAAAAPAEQRRPWLDRLKSGLRRTGSGITQVFTGTRIDEALYEE
LEAALLMADAGMPATQHLLADLKRRVKEHKATDPAAVKLLLVDALAELLAPLQRPLVLGETRPTVIMVAGVNGAGKTTSI
GKLTRHLADGGLKVLLAAADTFRAAAKEQLAVWADRNRVEIVSQEGGDPAAVSFDAVKAGQARGCDVVVADTAGRLPTQA
HLMEELRKIKRTIGKAMDGAPHEVLLVVDGNTGQNALAQVKAFDAVLGLTGLVVTKLDGTAKGGVLAAIALWSRERDGAS
PVPVYFIGVGEKLADLETFDAREFSQALLT

Nucleotide

• Length: 1053 bp

>NTDB_id=27424 MPE_RS16265 WP_011830798.1 3427258..3428310(-) (pilA) [Methylibium petroleiphilum PM1]
ATGTTCAGTTTCTTCAAGAAGAAGGCGCCGGTCGTGGCGCCACCGCCGACCGGCCCCGAGGCCCCAGCAGCATCCGCACC
GTCTCCCGGGCCTGCTGCCGAAGTCCGCGTGGCTGCTGCGGCGCCAGCCGAACAGCGCCGCCCCTGGCTCGACCGGCTCA
AGAGCGGGCTGCGGCGCACCGGATCCGGCATCACGCAGGTGTTCACCGGCACGCGCATCGACGAGGCGCTGTACGAGGAA
CTCGAGGCGGCGCTGCTGATGGCCGACGCCGGCATGCCCGCCACGCAGCACCTGCTCGCCGACCTGAAGCGGCGCGTCAA
GGAGCACAAGGCCACCGATCCTGCCGCGGTGAAGTTGTTGCTGGTCGACGCGCTGGCCGAATTGCTTGCGCCCCTGCAGC
GGCCGCTGGTGCTGGGCGAGACGCGGCCGACGGTGATCATGGTTGCGGGGGTCAACGGCGCGGGCAAGACGACCAGCATC
GGCAAGCTCACGCGCCACCTTGCCGACGGCGGCCTGAAGGTGCTGCTGGCGGCGGCCGACACCTTCCGCGCAGCGGCGAA
GGAGCAACTCGCGGTGTGGGCGGACCGCAACCGCGTCGAGATCGTCAGCCAGGAAGGCGGCGACCCGGCCGCGGTGAGCT
TCGATGCCGTGAAGGCCGGGCAGGCCCGCGGCTGCGATGTGGTCGTCGCCGACACCGCCGGCCGCCTGCCGACGCAGGCG
CACCTGATGGAAGAGCTGCGCAAGATCAAGCGCACCATCGGCAAGGCCATGGACGGCGCGCCGCACGAGGTGCTGTTGGT
GGTGGACGGCAACACCGGGCAGAACGCGCTGGCCCAGGTCAAGGCCTTCGATGCCGTGCTGGGGCTGACCGGGCTGGTCG
TCACCAAGCTCGACGGCACTGCCAAGGGCGGGGTGCTGGCGGCGATCGCGCTGTGGTCGCGCGAGCGTGACGGCGCCTCG
CCGGTGCCGGTCTATTTCATCGGCGTGGGCGAGAAGCTCGCGGATCTGGAGACTTTCGACGCGCGCGAGTTCTCGCAGGC
GCTGCTGACCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (143-349)

• SRP54-type protein, helical bundle domain (SRP54_N) (61-125)

3D structure

• AlphaFold DB: A2SKT6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	54.328	95.714	0.52