Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MPE_RS00730 Genome accession   NC_008825
Coordinates   155771..157294 (-) Length   507 a.a.
NCBI ID   WP_011827749.1    Uniprot ID   W7W998
Organism   Methylibium petroleiphilum PM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 150771..162294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPE_RS00705 (Mpe_A0145) - 151586..152191 (+) 606 WP_011827746.1 LemA family protein -
  MPE_RS00710 (Mpe_A0146) - 152199..153116 (+) 918 WP_011827747.1 YgcG family protein -
  MPE_RS00715 - 153116..153625 (+) 510 WP_041929485.1 TPM domain-containing protein -
  MPE_RS00720 - 153664..154434 (+) 771 WP_051495971.1 DUF4399 domain-containing protein -
  MPE_RS00725 - 154452..155676 (-) 1225 Protein_146 class I SAM-dependent RNA methyltransferase -
  MPE_RS00730 (Mpe_A0148) rpoN/rpoN1 155771..157294 (-) 1524 WP_011827749.1 RNA polymerase factor sigma-54 Machinery gene
  MPE_RS00735 (Mpe_A0149) lptB 157294..158049 (-) 756 WP_011827750.1 LPS export ABC transporter ATP-binding protein -
  MPE_RS00740 (Mpe_A0150) lptA 158046..158660 (-) 615 WP_148210864.1 lipopolysaccharide transport periplasmic protein LptA -
  MPE_RS00745 (Mpe_A0151) - 158768..159412 (-) 645 WP_011827752.1 thiol:disulfide interchange protein DsbA/DsbL -
  MPE_RS00750 (Mpe_A0152) - 159472..160104 (-) 633 WP_011827753.1 SPOR domain-containing protein -
  MPE_RS00755 (Mpe_A0153) argS 160116..161801 (-) 1686 WP_011827754.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 56447.02 Da        Isoelectric Point: 4.7495

>NTDB_id=27412 MPE_RS00730 WP_011827749.1 155771..157294(-) (rpoN/rpoN1) [Methylibium petroleiphilum PM1]
MKPSLQVRFSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVEEDAPTPFDAPVERATATERQADDAWEGSGS
EVAADPEPVAVDAAEFGTTEREDWENGTEREDFDGIRETPGKAGNNDSDEFDPMERSSAGVSLQDHLRDQLRGMRLSDED
RGAVMVLIESLDEDGYLADPLEEIAQRLAGDEDDIAVEELLDRLRCALKWLHNLEPLGVGARDLSECLTLQLRAGPRCEA
QMIAILICKYHLELLARRDGKKLMAATGADEELLKAAQALIVRCEPKPGRPFTKAEANIIVPDVIVQKAGRGWRVVLNPD
VMPKLRINDLYAQAIKQQRGARTESGAGLSSRLQEARWFMKNILQRFDTIQRVSQAIVERQKAFFSHGAIAMKPLVLREI
ADELGLHESTISRVTTAKYMSTPYGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVSAEDAKKPLSDSQLSSMLEEQG
IQVARRTVAKYREALKIAPANLRRTMM

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=27412 MPE_RS00730 WP_011827749.1 155771..157294(-) (rpoN/rpoN1) [Methylibium petroleiphilum PM1]
ATGAAGCCTTCCCTGCAGGTCCGTTTTTCGCAGCATCTGGCGCTCACGCCGCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTCGAGCTTCACCAGGAAGTCGAGCAGATGCTGGAGCAGAACCCCTTCCTCGAAGTGGAGGAAGATGCGC
CGACCCCGTTCGACGCGCCGGTGGAGCGCGCCACGGCCACGGAGCGCCAGGCCGATGACGCCTGGGAGGGCTCGGGGTCC
GAGGTGGCCGCCGACCCCGAGCCGGTCGCGGTGGATGCGGCCGAGTTCGGCACCACCGAGCGCGAGGACTGGGAGAACGG
CACCGAGCGCGAGGACTTCGACGGCATCCGCGAGACGCCCGGCAAGGCCGGCAACAACGACAGCGACGAGTTCGACCCCA
TGGAGCGCAGCAGCGCCGGGGTGAGCCTGCAGGACCACCTGCGCGACCAGTTGCGCGGCATGCGCCTGAGCGACGAGGAC
CGCGGCGCGGTGATGGTGCTGATCGAATCGCTCGACGAGGACGGCTACCTGGCCGACCCCCTGGAAGAGATCGCCCAGCG
CCTGGCCGGCGACGAGGACGACATCGCGGTCGAGGAGCTGCTCGACCGCCTGCGCTGCGCGCTGAAGTGGCTGCACAACC
TGGAGCCGCTGGGCGTCGGTGCGCGCGACCTGTCGGAGTGCCTGACGCTGCAGCTGCGGGCCGGACCGCGCTGCGAGGCG
CAGATGATCGCGATCCTGATCTGCAAGTACCACCTCGAGTTGCTGGCGCGGCGCGACGGCAAGAAGCTGATGGCGGCCAC
CGGCGCCGACGAGGAGCTGCTGAAGGCCGCGCAGGCGCTGATCGTGCGCTGCGAGCCCAAGCCCGGCCGGCCCTTCACCA
AGGCCGAGGCCAACATCATCGTGCCCGATGTCATCGTGCAGAAGGCCGGCCGCGGCTGGCGCGTGGTGCTCAACCCCGAC
GTGATGCCCAAGCTGCGCATCAACGACCTCTACGCCCAGGCCATCAAGCAGCAGCGGGGCGCGCGCACCGAATCGGGCGC
GGGCCTGAGCTCGCGGCTGCAGGAGGCGCGCTGGTTCATGAAGAACATCCTGCAGCGCTTCGACACCATCCAGCGCGTGT
CGCAGGCCATCGTCGAGCGGCAGAAGGCCTTCTTCAGCCACGGCGCGATCGCGATGAAGCCACTGGTGCTGCGCGAGATC
GCCGACGAGCTGGGTCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGTCCACGCCCTACGGCACCTT
CGAGCTGAAGTATTTCTTCGGCTCCTCGCTCAACACCGAGGCCGGTGGCAATGCGTCGAGCACCGCGGTGCGTGCGCTGA
TCAAGCAGCTGGTCAGTGCCGAGGATGCCAAGAAGCCGCTGTCGGACAGCCAGCTCAGCAGCATGCTGGAAGAGCAGGGC
ATCCAGGTGGCGCGCCGCACGGTGGCGAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCCAACCTGCGGCGCACGATGAT
GTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-331)

Sigma-54, DNA binding domain (Sigma54_DBD)

(350-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7W998

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.757

100

0.55

Multiple sequence alignment