Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MC81_RS22920 Genome accession   NZ_CP026973
Coordinates   4932083..4933528 (-) Length   481 a.a.
NCBI ID   WP_063961223.1    Uniprot ID   -
Organism   Achromobacter insolitus strain FDAARGOS_88     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4927083..4938528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MC81_RS22895 (MC81_22895) - 4928111..4928794 (+) 684 WP_170946311.1 hypothetical protein -
  MC81_RS22900 (MC81_22900) bfr 4928872..4929348 (-) 477 WP_042795741.1 bacterioferritin -
  MC81_RS22905 (MC81_22905) - 4929735..4930649 (-) 915 WP_042795742.1 DMT family transporter -
  MC81_RS22910 (MC81_22910) - 4930780..4931637 (+) 858 WP_042795744.1 LysR substrate-binding domain-containing protein -
  MC81_RS22915 (MC81_22915) - 4931668..4931949 (-) 282 WP_042795745.1 bacterioferritin-associated ferredoxin -
  MC81_RS22920 (MC81_22920) rpoN/rpoN1 4932083..4933528 (-) 1446 WP_063961223.1 RNA polymerase factor sigma-54 Machinery gene
  MC81_RS22925 (MC81_22925) - 4933527..4934156 (+) 630 WP_042795747.1 hypothetical protein -
  MC81_RS22930 (MC81_22930) leuA 4934666..4936378 (+) 1713 WP_042795748.1 2-isopropylmalate synthase -
  MC81_RS22935 (MC81_22935) - 4936480..4937808 (-) 1329 WP_042795749.1 amidase -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52411.65 Da        Isoelectric Point: 4.8020

>NTDB_id=274091 MC81_RS22920 WP_063961223.1 4932083..4933528(-) (rpoN/rpoN1) [Achromobacter insolitus strain FDAARGOS_88]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLERQDEAPSAEAPAESDRESSVQDDEP
AAERETPVDEMPGSGGVYPDEDSSRPEAARPDTLREHLLGQLGLTRAAPRDVALAGLLIDELDENGYLGSPLEEILDWLP
AELEPDLDELKAALSLLQSFDPPGIGARDMAECLLLQLRNPDLEQLPEAADAAVLACARQICAQHLALLATGNAARLCAA
VGCDEATFRSAHALIRRLEPRPGRAWTVPAADYAVPDVIVRKTRGGWQVTLNSAAVPKLQINGLYAQMLGNQKESAHAGL
QSQLQQARWMIRNVEQRFDTILRVAQAIVQHQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAIQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=274091 MC81_RS22920 WP_063961223.1 4932083..4933528(-) (rpoN/rpoN1) [Achromobacter insolitus strain FDAARGOS_88]
ATGCAAAATATTGTACCTATGACCCGCCCAATTTTAGAACTGCGACCTGGTCAGCATTTGACGCTGACGCCACAACTGCA
GCAATCGATCCGATTGTTGCAGTTGTCCACGCTCGACCTCGAAGCCGAGATCTCGCAAGTGCTGGCGGAGAACCCGCTGC
TGGAGCGCCAGGACGAAGCGCCGTCGGCCGAGGCGCCAGCGGAATCCGACCGCGAATCCTCCGTGCAGGACGACGAGCCC
GCCGCTGAGCGCGAGACGCCAGTGGATGAAATGCCGGGTTCCGGCGGCGTCTATCCTGACGAGGACTCCAGCCGGCCCGA
GGCCGCGCGCCCTGACACGCTGCGCGAGCATCTGCTGGGCCAATTGGGGCTGACGCGGGCCGCCCCGAGGGACGTCGCGC
TGGCCGGCCTCCTGATCGACGAACTGGACGAGAACGGGTACCTGGGATCGCCGCTGGAGGAAATCCTGGACTGGTTGCCG
GCGGAATTGGAGCCGGACCTGGATGAGCTGAAGGCGGCCCTGTCGCTGCTCCAGTCCTTCGATCCCCCCGGCATCGGCGC
GCGCGACATGGCGGAATGCCTGCTGTTGCAGCTGCGCAACCCGGACCTGGAACAACTGCCTGAAGCGGCGGATGCCGCGG
TATTGGCCTGTGCACGCCAGATCTGCGCGCAGCACCTGGCCCTGTTGGCGACGGGCAACGCCGCGCGCCTGTGCGCCGCC
GTGGGCTGCGATGAAGCCACGTTCCGCTCGGCCCATGCGCTGATCCGGCGCCTGGAGCCGCGTCCGGGGCGCGCCTGGAC
CGTGCCCGCGGCCGACTACGCCGTGCCCGACGTCATCGTGCGCAAGACCCGCGGCGGCTGGCAGGTGACCCTGAACAGCG
CCGCCGTGCCCAAGCTGCAGATCAACGGGCTCTACGCCCAGATGCTGGGGAACCAAAAGGAATCCGCGCACGCGGGGCTG
CAATCGCAGCTGCAGCAGGCACGCTGGATGATCCGCAATGTGGAGCAGCGTTTCGACACCATCCTGCGCGTAGCCCAGGC
CATCGTGCAGCACCAGACGGCTTTTTTCAGCCAGGGGCCTGCCGCCATGCGGCCGCTCATCCTGAAGGACATCGCTGGCG
AGCTCGGTTTGCACGAATCCACGATTTCACGGGCCACAACACAGAAGTACATGCTCACTCCGTTTGGCACATTGGAGCTC
AAGCGCTTCTTTGGCACGGGCGTGTCGACGGACGGCGGGGACGCCACCTCGGCCACGGCGATACAGACTTTCATCCGGCA
AATGGTGGCGGAGGAGAACCGGGCCAAGCCTTTGTCCGATAGCCAGATCATGCAAAAGCTGGCCGATCAGGGGATAGTCA
TCGCCCGTCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATAGCCCCGGCGGCATTGCGCAAGGCCCAGGCGGCCGCC
CGTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.111

100

0.503

Multiple sequence alignment