Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   P9303_RS00115 Genome accession   NC_008820
Coordinates   23070..24986 (-) Length   638 a.a.
NCBI ID   WP_011824709.1    Uniprot ID   -
Organism   Prochlorococcus marinus str. MIT 9303     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18070..29986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9303_RS00095 (P9303_00151) msrB 18956..19462 (+) 507 WP_011824706.1 peptide-methionine (R)-S-oxide reductase MsrB -
  P9303_RS00100 (P9303_00161) - 19389..20711 (+) 1323 WP_011824707.1 NAD(P)/FAD-dependent oxidoreductase -
  P9303_RS00105 (P9303_00171) - 20686..21966 (-) 1281 WP_011824708.1 type II secretion system F family protein -
  P9303_RS00110 (P9303_00181) pilT 21983..23059 (-) 1077 WP_011129398.1 type IV pilus twitching motility protein PilT Machinery gene
  P9303_RS00115 (P9303_00191) pilB/pilB1 23070..24986 (-) 1917 WP_011824709.1 GspE/PulE family protein Machinery gene
  P9303_RS00120 (P9303_00201) grpE 24985..25698 (+) 714 WP_011824710.1 nucleotide exchange factor GrpE -
  P9303_RS00125 (P9303_00211) dnaJ 25743..26879 (+) 1137 WP_011824711.1 molecular chaperone DnaJ -
  P9303_RS00130 (P9303_00221) - 26876..27121 (+) 246 WP_011824712.1 sulfurtransferase TusA family protein -
  P9303_RS00135 (P9303_00231) rsgA 27108..28073 (+) 966 WP_011824713.1 ribosome small subunit-dependent GTPase A -
  P9303_RS00140 (P9303_00241) - 28030..28371 (-) 342 WP_011824714.1 YbaB/EbfC family nucleoid-associated protein -
  P9303_RS00145 (P9303_00251) murB 28396..29319 (-) 924 WP_011824715.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 638 a.a.        Molecular weight: 70294.20 Da        Isoelectric Point: 6.4055

>NTDB_id=27400 P9303_RS00115 WP_011824709.1 23070..24986(-) (pilB/pilB1) [Prochlorococcus marinus str. MIT 9303]
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVSVLSQEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISLLQSQGFSTELRLALASDISQLLAPMRSEQHGESASKSKATK
PLAQTPTSLLAGFSAEGVLEEDPEEQARLASSVEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVYEVLRMNEELATAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDGWLECPYCLTARQ

Nucleotide


Download         Length: 1917 bp        

>NTDB_id=27400 P9303_RS00115 WP_011824709.1 23070..24986(-) (pilB/pilB1) [Prochlorococcus marinus str. MIT 9303]
ATGCTGACCGTTTGCAAATTCACGTCTAGACATGTTGATGGGAGTAGCACACCTCCCACAAGCGGCGGAAGCCCGCACCT
CCAATTTCATTTCTTCTTCGTGACCCAGGGCCGACCAATCCCCAAAGCAACCAACAGCATCCAGCAGCGACTGGAACTGG
AACTGCTCCTACAAGTCAGTGTGCTTAGCCAAGAAGAACTTGTTGTCGGAGTGGAGCTAATGGCCAACCACACCACTCTT
GACATCTCAACGTGGCAACAATTCCAGGCCCTGCCAATCAATATGCACAACCAACATTTGGTTGTAGCAATCTCGGACCA
ATGCAATGAGCAAACCAAAAATCAACTGATCTCATTGCTGCAGTCGCAAGGCTTCAGCACAGAATTACGTCTCGCGCTGG
CATCCGACATCAGCCAGCTGCTTGCACCAATGAGATCTGAGCAGCACGGTGAATCTGCATCCAAGTCAAAAGCAACAAAG
CCACTTGCACAAACACCAACCTCCCTGCTGGCAGGATTTAGTGCCGAAGGCGTGCTCGAAGAAGATCCTGAAGAACAAGC
CAGACTTGCTAGTTCCGTAGAGGATTTGGAGTCCAGCTTGATGGACTCAGACAGCTCACCAGTCATCAACCTGGTGGACC
GCATACTGCTAGAGGCACTGCAAACAGAAGCCAGCGACGTACACGTTGAGCCGCAACAAGACGGACTACAGATCCGCTTC
CGTCAAGACGGTGTTTTGCAGCGCTATATCGAACCCCTCCCGAGCCGGTTGATCCCTGCTGTTACCTCACGCTTCAAGAT
CATGGCCGACCTAGACATCGCAGAACGGCGCATGGCTCAGGACGGTCGCATTCGTCGCACATATCGCAATCGTATGGTCG
ATTTCAGGGTTAATAGCCTGCCAAGCCGTTACGGGGAAAAAATCTGTCTGCGACTCCTCGACAGCAGTGCTCCACAACTC
GGGCTAGACAAACTGATCAGCAACCCAAGTGCTCTCTCTCTCGTACGCAACTTGGGCTCTAAGCCCTTCGGCATGATCCT
TGTGACAGGTCCAACAGGATCAGGCAAGTCAACAACCCTTTACTCCCTACTGGCAGAACGCAACGAGCCTGGTATCAACA
TTTCCACCGTAGAAGATCCCATCGAATACACCCTCCCTGGGATTACCCAATGTCAAGTGAACAGGGAAAAAGGCTTCGAT
TTCAGTACTGCACTGAGAGCCTTCATGCGTCAAGACCCAGACGTTCTACTGGTCGGTGAAACCCGTGACCTAGAAACCGC
AAAAACCGCCATCGAAGCCGCACTGACTGGTCATCTGGTGCTAACCACACTGCATTGCAATGACGCATCAAGTGCGATCG
CCCGCCTCAACGAAATGGGCGTGGAACCATTCATGGTGAGCGCCTCGTTGATTGGCATCGTCTCTCAAAGACTTCTACGA
AGAGTGTGCCGTTCTTGTCGTAAGTCCTATCACCCAACTGAAAAAGAACTGGGGCGATTCGGATTGATGGCCCATACAGA
AACTGGCGTGACCTTTTTCAAGGCCCACCATCACGGTCAAGAAAAACAGCCGTGCCCCAACTGTCAAGGCAGCGGCTACA
AGGGCCGAGTTGGTGTCTATGAAGTACTGCGCATGAATGAAGAGCTCGCTACAGCCGTCGCCAAAGGTGCCACTACTGAT
TTAGTAAGACGTCTGGCGCTCGAGGCTGGTATGAAAACTCTGTTGGGCTACAGCCTTGACCTGGTGCGAGAAGGCCACAC
CACCCTTGAGGAAGTGGGCCGAATGATCCTCACCGATTCCGGATTGGAATCAGAGCGCCGCGCCAGAGCGCTTAGCACTC
TCACCTGCAACGTCTGCGGGGCAGGCCTCCAAGACGGCTGGCTGGAATGTCCCTACTGTCTAACCGCTCGCCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(215-481)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

53.642

94.671

0.508

Multiple sequence alignment