Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CCU01_RS00625 Genome accession   NZ_CP031919
Coordinates   117054..117722 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O145:NM strain FWSEC0002     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 112054..122722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCU01_RS00595 (CCU01_000615) yicC 112073..112936 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  CCU01_RS00600 (CCU01_000625) rph 113063..113779 (+) 717 WP_001247093.1 ribonuclease PH -
  CCU01_RS00605 (CCU01_000630) pyrE 113845..114486 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CCU01_RS00610 (CCU01_000635) slmA 114523..115119 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CCU01_RS00615 (CCU01_000640) dut 115226..115684 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CCU01_RS00620 (CCU01_000645) coaBC 115662..116882 (-) 1221 WP_000050121.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CCU01_RS00625 (CCU01_000650) radC 117054..117722 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CCU01_RS00630 (CCU01_000655) rpmB 117939..118175 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CCU01_RS00635 (CCU01_000660) rpmG 118196..118363 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CCU01_RS00640 (CCU01_000665) mutM 118461..119270 (+) 810 WP_001114545.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CCU01_RS00645 (CCU01_000670) coaD 119309..119788 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CCU01_RS00650 (CCU01_000675) waaA 119796..121073 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CCU01_RS00655 (CCU01_000680) rfaQ 121486..122544 (+) 1059 WP_000360416.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=273554 CCU01_RS00625 WP_001297375.1 117054..117722(+) (radC) [Escherichia coli O145:NM strain FWSEC0002]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=273554 CCU01_RS00625 WP_001297375.1 117054..117722(+) (radC) [Escherichia coli O145:NM strain FWSEC0002]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment