Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CCU02_RS00425 Genome accession   NZ_CP031916
Coordinates   84280..84948 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli O45:H2 strain FWSEC0003     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 79280..89948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCU02_RS00395 (CCU02_000425) yicC 79299..80162 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  CCU02_RS00400 (CCU02_000435) rph 80289..81005 (+) 717 WP_001247093.1 ribonuclease PH -
  CCU02_RS00405 (CCU02_000440) pyrE 81071..81712 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  CCU02_RS00410 (CCU02_000445) slmA 81749..82345 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CCU02_RS00415 (CCU02_000450) dut 82452..82910 (-) 459 WP_000976074.1 dUTP diphosphatase -
  CCU02_RS00420 (CCU02_000455) coaBC 82888..84108 (-) 1221 WP_000050117.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CCU02_RS00425 (CCU02_000460) radC 84280..84948 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  CCU02_RS00430 (CCU02_000465) rpmB 85165..85401 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CCU02_RS00435 (CCU02_000470) rpmG 85422..85589 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CCU02_RS00440 (CCU02_000475) mutM 85687..86496 (+) 810 WP_001114546.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CCU02_RS00445 (CCU02_000480) coaD 86535..87014 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  CCU02_RS00450 (CCU02_000485) waaA 87022..88299 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CCU02_RS00455 (CCU02_000490) rfaQ 88712..89770 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=273474 CCU02_RS00425 WP_001298959.1 84280..84948(+) (radC) [Escherichia coli O45:H2 strain FWSEC0003]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=273474 CCU02_RS00425 WP_001298959.1 84280..84948(+) (radC) [Escherichia coli O45:H2 strain FWSEC0003]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment