Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VEIS_RS23060 Genome accession   NC_008786
Coordinates   5255047..5256168 (-) Length   373 a.a.
NCBI ID   WP_011812432.1    Uniprot ID   A1WS47
Organism   Verminephrobacter eiseniae EF01-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5250047..5261168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEIS_RS23045 (Veis_4758) ettA 5251312..5252973 (+) 1662 WP_011812430.1 energy-dependent translational throttle protein EttA -
  VEIS_RS31775 (Veis_4759) - 5253112..5253657 (+) 546 Protein_4941 DUF1631 family protein -
  VEIS_RS23055 (Veis_4760) - 5253741..5255084 (+) 1344 WP_011812431.1 DUF1631 family protein -
  VEIS_RS23060 (Veis_4761) pilA 5255047..5256168 (-) 1122 WP_011812432.1 signal recognition particle-docking protein FtsY Machinery gene
  VEIS_RS23065 (Veis_4762) - 5256198..5257637 (+) 1440 WP_011812433.1 pitrilysin family protein -
  VEIS_RS23070 (Veis_4763) - 5257657..5259003 (+) 1347 WP_011812434.1 pitrilysin family protein -
  VEIS_RS23075 (Veis_4764) - 5259210..5259956 (+) 747 WP_049774111.1 RsmD family RNA methyltransferase -
  VEIS_RS23080 (Veis_4765) coaD 5260025..5260528 (+) 504 WP_011812436.1 pantetheine-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 38399.14 Da        Isoelectric Point: 7.4706

>NTDB_id=27324 VEIS_RS23060 WP_011812432.1 5255047..5256168(-) (pilA) [Verminephrobacter eiseniae EF01-2]
MFSFFKKKPAPVPADAPAAASAAAAEPGAEPAGLGWLRNPFASPAPETPAAQPAAPAVAAPAAAPPAAASASPAGRTGWL
LRLKSGLRKTGSSISTVFTGTQIDDALYEELETALLLADTGVKATGHLLAELKRRAKERRATDPAAVKALLAEALAELLR
PLEKTLVIGAHRPTVIMVAGVNGAGKTTSIGKLTKHLADAGATVLLAAADTFRAAAREQLGVWADRNMVEIVGQEGGDPA
AVGFDAVSAGKARGKDVVLVDTAGRLPTQLHLMQELAKIRRVIGKADAGAPHEVLLVLDGNTGQNALAQVRAFDDALQLT
GLIVTKLDGTAKGGVLAAIAQERPIPVYFIGVGEKLEDLETFNAREFSQALLD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=27324 VEIS_RS23060 WP_011812432.1 5255047..5256168(-) (pilA) [Verminephrobacter eiseniae EF01-2]
ATGTTCAGTTTTTTCAAAAAAAAACCCGCGCCCGTGCCGGCCGACGCACCTGCTGCGGCCAGCGCGGCGGCGGCCGAACC
TGGCGCCGAGCCTGCGGGCCTGGGCTGGCTGCGCAACCCGTTTGCGTCCCCGGCGCCCGAAACCCCGGCAGCACAGCCGG
CCGCACCAGCGGTGGCGGCCCCTGCTGCGGCACCTCCTGCTGCGGCCAGCGCGTCCCCGGCCGGGCGCACCGGCTGGCTG
CTCCGTCTCAAGTCCGGTCTGCGCAAGACCGGCAGCAGCATCAGCACGGTTTTTACCGGCACGCAGATCGATGATGCGCT
GTATGAAGAACTGGAAACGGCCCTGCTGCTGGCCGACACCGGCGTCAAGGCCACCGGCCATCTGCTGGCCGAGCTCAAGC
GCCGCGCCAAGGAGCGCCGGGCCACCGACCCGGCAGCCGTCAAGGCGCTGCTGGCCGAGGCCCTGGCCGAGTTGCTGCGC
CCGCTGGAAAAAACCCTGGTCATCGGCGCGCACCGCCCCACGGTGATCATGGTGGCTGGCGTCAATGGCGCGGGCAAGAC
CACCTCGATCGGCAAGCTCACCAAGCATCTGGCGGATGCCGGCGCTACGGTGCTGCTGGCGGCGGCCGACACCTTTCGCG
CTGCGGCGCGTGAGCAGTTGGGCGTCTGGGCCGACCGCAACATGGTCGAGATCGTCGGCCAGGAGGGCGGCGATCCGGCC
GCCGTGGGCTTCGATGCCGTCAGCGCCGGCAAGGCGCGCGGCAAGGACGTGGTGCTGGTGGACACCGCTGGCCGCCTGCC
CACGCAGTTGCACCTGATGCAGGAACTGGCAAAAATCCGCCGCGTGATTGGCAAGGCCGACGCCGGCGCGCCCCACGAGG
TGCTGCTGGTGCTCGACGGCAACACCGGCCAGAACGCATTGGCCCAGGTGCGCGCCTTCGATGACGCGCTGCAACTGACC
GGGCTGATCGTGACCAAGCTCGACGGCACGGCCAAGGGCGGCGTGCTGGCCGCGATTGCGCAAGAGCGCCCGATACCGGT
CTATTTCATCGGCGTGGGCGAAAAGCTCGAAGACCTGGAGACCTTCAACGCGCGCGAGTTTTCCCAGGCCCTTTTGGACT
GA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(173-372)

SRP54-type protein, helical bundle domain (SRP54_N)

(92-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1WS47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.681

100

0.544

Multiple sequence alignment