Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   B9X35_RS00340 Genome accession   NZ_CP031910
Coordinates   72115..72783 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli O121:H19 strain FWSEC0006     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 67115..77783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9X35_RS00310 (B9X35_000340) yicC 67134..67997 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  B9X35_RS00315 (B9X35_000350) rph 68124..68840 (+) 717 WP_001247089.1 ribonuclease PH -
  B9X35_RS00320 (B9X35_000355) pyrE 68906..69547 (+) 642 WP_000806180.1 orotate phosphoribosyltransferase -
  B9X35_RS00325 (B9X35_000360) slmA 69584..70180 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  B9X35_RS00330 (B9X35_000365) dut 70287..70745 (-) 459 WP_000976070.1 dUTP diphosphatase -
  B9X35_RS00335 (B9X35_000370) coaBC 70723..71943 (-) 1221 WP_000050159.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  B9X35_RS00340 (B9X35_000375) radC 72115..72783 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  B9X35_RS00345 (B9X35_000380) rpmB 73000..73236 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  B9X35_RS00350 (B9X35_000385) rpmG 73257..73424 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  B9X35_RS00355 (B9X35_000390) mutM 73522..74331 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  B9X35_RS00360 (B9X35_000395) coaD 74370..74849 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  B9X35_RS00365 (B9X35_000400) waaA 74857..76134 (-) 1278 WP_000891575.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  B9X35_RS00370 (B9X35_000405) rfaQ 76547..77605 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=273236 B9X35_RS00340 WP_001298959.1 72115..72783(+) (radC) [Escherichia coli O121:H19 strain FWSEC0006]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=273236 B9X35_RS00340 WP_001298959.1 72115..72783(+) (radC) [Escherichia coli O121:H19 strain FWSEC0006]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment