Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VEIS_RS02830 Genome accession   NC_008786
Coordinates   647085..648665 (+) Length   526 a.a.
NCBI ID   WP_011808376.1    Uniprot ID   A1WFF5
Organism   Verminephrobacter eiseniae EF01-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 642085..653665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEIS_RS02805 (Veis_0573) argS 642383..644092 (+) 1710 WP_011808371.1 arginine--tRNA ligase -
  VEIS_RS02810 (Veis_0574) - 644096..644773 (+) 678 WP_011808372.1 SPOR domain-containing protein -
  VEIS_RS02815 (Veis_0575) - 644833..645504 (+) 672 WP_011808373.1 thiol:disulfide interchange protein DsbA/DsbL -
  VEIS_RS02820 (Veis_0576) lptA 645650..646303 (+) 654 WP_011808374.1 lipopolysaccharide transport periplasmic protein LptA -
  VEIS_RS02825 (Veis_0577) lptB 646300..647085 (+) 786 WP_049773797.1 LPS export ABC transporter ATP-binding protein -
  VEIS_RS02830 (Veis_0578) rpoN/rpoN1 647085..648665 (+) 1581 WP_011808376.1 RNA polymerase factor sigma-54 Machinery gene
  VEIS_RS02835 (Veis_0579) - 648665..649957 (+) 1293 WP_011808377.1 class I SAM-dependent RNA methyltransferase -
  VEIS_RS25360 - 650215..650367 (-) 153 WP_157048376.1 hypothetical protein -
  VEIS_RS02840 (Veis_0581) - 650375..650902 (-) 528 WP_011808378.1 CinA family protein -
  VEIS_RS02845 (Veis_0582) - 650902..651429 (-) 528 WP_011808379.1 phosphatidylglycerophosphatase A -
  VEIS_RS02850 (Veis_0583) thiL 651426..652430 (-) 1005 WP_041950460.1 thiamine-phosphate kinase -
  VEIS_RS02855 (Veis_0584) - 652460..653584 (-) 1125 WP_011808381.1 YbdK family carboxylate-amine ligase -

Sequence

Protein


Download         Length: 526 a.a.        Molecular weight: 57857.11 Da        Isoelectric Point: 4.9838

>NTDB_id=27308 VEIS_RS02830 WP_011808376.1 647085..648665(+) (rpoN/rpoN1) [Verminephrobacter eiseniae EF01-2]
MKPGLSLRVSQHLTLTPQLQQSIRLLQLSTLELTQEIEQMLDENPFLERMSDEAPREEFGLDSADTPAQADDYSADEVLF
SAATGSSAEALPDADTPGSAADEPDWGGDGSVEMAPSDAEWGGDAPASQHNDAEGDGIEATELAQSEESLAAFLHRQALA
LRLSEIDRAALYFLIESLNDDGYLEDSLEELASALAGPQNPEQIEELVHRFTVAQRLLQSLEPVGVGARNLAECLTLQLN
ALARERAPAADPQTVQTALRICQQPLQMLARRDLRRLTQVCGDSQEGTRAAMALIARLEPHPGRRFANVQRNVIVPDVIV
RKTGRANGRDGRQHFIVQINPDVLPRLRVHDIYAGALRGHRGGAGHQGMQQRLQEARWFIKNIQQRFDTILRVSRAIVER
QKNFFTHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPIGTYELKYFFGSGLGTETGGNASSTAVRALIRQFVAA
ESQAKPLSDSQLADLLKEQGIECARRTVAKYREALKIAPANLRRTL

Nucleotide


Download         Length: 1581 bp        

>NTDB_id=27308 VEIS_RS02830 WP_011808376.1 647085..648665(+) (rpoN/rpoN1) [Verminephrobacter eiseniae EF01-2]
ATGAAGCCCGGTCTGTCACTGCGCGTCTCGCAGCACCTCACACTGACCCCTCAGTTGCAGCAGTCGATCCGGCTGCTGCA
ACTGTCCACGCTCGAACTGACGCAGGAAATCGAGCAGATGCTCGATGAAAACCCGTTTCTCGAACGCATGTCCGACGAGG
CCCCGCGCGAGGAGTTCGGCCTGGACAGCGCCGACACGCCAGCGCAAGCCGATGACTACAGCGCCGACGAAGTGCTCTTT
TCCGCCGCAACCGGCAGCAGCGCCGAAGCGCTCCCTGACGCCGACACCCCGGGCAGCGCTGCGGACGAGCCCGACTGGGG
CGGCGACGGCAGCGTGGAGATGGCGCCCAGCGATGCCGAATGGGGCGGCGACGCGCCGGCGAGCCAGCACAACGACGCCG
AAGGCGACGGGATCGAAGCCACCGAACTGGCCCAGAGCGAAGAATCGCTGGCCGCTTTCTTGCACCGCCAGGCGCTGGCG
CTGCGCCTGTCCGAAATCGACCGCGCCGCGCTGTACTTCCTGATCGAATCGCTCAACGACGACGGCTACCTGGAAGACTC
GCTCGAAGAACTGGCCAGCGCCCTGGCCGGCCCGCAGAACCCGGAGCAAATCGAAGAACTGGTGCACCGCTTCACCGTCG
CCCAACGCCTGCTGCAAAGCCTGGAACCGGTGGGCGTGGGCGCGCGCAATCTGGCCGAATGCCTGACGCTACAGCTCAAC
GCCCTGGCACGGGAGCGGGCGCCAGCCGCCGACCCGCAGACCGTGCAAACCGCCCTGCGCATCTGCCAGCAGCCGCTCCA
AATGCTCGCCCGGCGCGACCTGCGCCGCCTGACCCAGGTCTGCGGCGACAGCCAGGAGGGCACCCGCGCCGCGATGGCCC
TGATCGCCCGCCTGGAGCCGCACCCGGGGCGCCGCTTTGCCAATGTGCAGCGCAACGTCATCGTGCCCGACGTGATCGTG
CGCAAAACCGGGCGCGCCAATGGGCGCGACGGCCGACAGCATTTCATCGTGCAGATCAACCCCGACGTGCTGCCCCGCCT
GCGCGTGCACGATATCTACGCCGGCGCGCTGCGCGGCCACAGGGGCGGCGCAGGCCACCAGGGCATGCAGCAACGCCTGC
AGGAAGCGCGCTGGTTCATCAAGAACATACAGCAGCGCTTCGACACCATCTTGCGCGTCTCGCGCGCCATCGTCGAGCGG
CAGAAAAACTTCTTCACCCACGGCGAACTGGCCATGCGGCCGCTGGTGCTGCGCGACATCGCCGACGAACTGGGCCTGCA
CGAATCGACCATCAGCCGCGTGACCACCGCCAAATACATGGCCACCCCGATAGGCACCTACGAACTGAAATACTTTTTCG
GCTCGGGCCTGGGCACCGAAACCGGCGGCAACGCATCGAGCACCGCCGTGCGCGCGCTGATCCGGCAATTCGTCGCTGCG
GAAAGCCAGGCCAAGCCCTTGTCCGACAGCCAACTCGCCGACCTGCTCAAAGAGCAGGGCATAGAGTGCGCGCGCCGCAC
CGTGGCCAAGTACCGCGAGGCGCTCAAGATCGCGCCGGCCAATCTGCGCCGGACGCTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(369-524)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-353)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1WFF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.036

100

0.515

Multiple sequence alignment