Detailed information NTG0027258

Bioinformatically predicted

Overview

• Name: radC
• Type: Machinery gene
• Locus tag: ECE24377A_RS22195
• Genome accession: NC_009801
• Coordinates: 4127827..4128495 (-)
• Length: 222 a.a.
• NCBI ID: WP_001297375.1
• Uniprot ID: P65957
• Organism: Escherichia coli O139:H28 str. E24377A
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 4122827..4133495

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECE24377A_RS22165 (EcE24377A_4133)	rfaQ	4123005..4124063 (-)	1059	WP_000360402.1	lipopolysaccharide core heptosyltransferase RfaQ	-
ECE24377A_RS22170 (EcE24377A_4134)	waaA	4124476..4125753 (+)	1278	WP_000891564.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
ECE24377A_RS22175 (EcE24377A_4135)	coaD	4125761..4126240 (+)	480	WP_001171866.1	pantetheine-phosphate adenylyltransferase	-
ECE24377A_RS22180 (EcE24377A_4136)	mutM	4126279..4127088 (-)	810	WP_001114533.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-
ECE24377A_RS22185 (EcE24377A_4137)	rpmG	4127186..4127353 (-)	168	WP_001051798.1	50S ribosomal protein L33	-
ECE24377A_RS22190 (EcE24377A_4138)	rpmB	4127374..4127610 (-)	237	WP_000091955.1	50S ribosomal protein L28	-
ECE24377A_RS22195 (EcE24377A_4139)	radC	4127827..4128495 (-)	669	WP_001297375.1	DNA repair protein RadC	Machinery gene
ECE24377A_RS22200 (EcE24377A_4140)	coaBC	4128667..4129887 (+)	1221	WP_000050115.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ECE24377A_RS22205 (EcE24377A_4141)	dut	4129865..4130323 (+)	459	WP_000976070.1	dUTP diphosphatase	-
ECE24377A_RS22210 (EcE24377A_4142)	slmA	4130430..4131026 (+)	597	WP_000818601.1	nucleoid occlusion factor SlmA	-
ECE24377A_RS22215 (EcE24377A_4143)	pyrE	4131063..4131704 (-)	642	WP_000806162.1	orotate phosphoribosyltransferase	-
ECE24377A_RS22220 (EcE24377A_4144)	rph	4131770..4132486 (-)	717	WP_001247093.1	ribonuclease PH	-
ECE24377A_RS22225 (EcE24377A_4145)	yicC	4132613..4133476 (+)	864	WP_000621336.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 222 a.a.
• Molecular weight: 25258.35 Da
• Isoelectric Point: 7.9748

>NTDB_id=27258 ECE24377A_RS22195 WP_001297375.1 4127827..4128495(-) (radC) [Escherichia coli O139:H28 str. E24377A]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide

• Length: 669 bp

>NTDB_id=27258 ECE24377A_RS22195 WP_001297375.1 4127827..4128495(-) (radC) [Escherichia coli O139:H28 str. E24377A]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

• RadC-like JAB domain (RadC) (103-220)

• UPF0758 N-terminal (UPF0758_N) (9-77)

3D structure

• AlphaFold DB: P65957

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radC	Haemophilus influenzae Rd KW20	49.55	100	0.496