Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   D1100_RS04850 Genome accession   NZ_CP031869
Coordinates   1053031..1054410 (+) Length   459 a.a.
NCBI ID   WP_017357656.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain WF83     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1048031..1059410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1100_RS04820 (D1100_04835) yacG 1048310..1048498 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1100_RS04825 (D1100_04840) coaE 1048480..1049118 (-) 639 WP_005617375.1 dephospho-CoA kinase -
  D1100_RS04830 (D1100_04845) - 1049140..1049778 (-) 639 WP_012478451.1 prepilin peptidase -
  D1100_RS04835 (D1100_04850) pilC 1049805..1051007 (-) 1203 WP_005617379.1 type II secretion system F family protein Machinery gene
  D1100_RS04840 (D1100_04855) pilB 1050991..1052385 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1100_RS04845 (D1100_04860) pilA 1052403..1052849 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1100_RS04850 (D1100_04865) radA/sms 1053031..1054410 (+) 1380 WP_017357656.1 DNA repair protein RadA Machinery gene
  D1100_RS04855 (D1100_04870) - 1054454..1054687 (-) 234 WP_005615190.1 YgjV family protein -
  D1100_RS04860 (D1100_04875) folA 1054814..1055302 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1100_RS04865 (D1100_04880) proB 1055389..1056480 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1100_RS04870 (D1100_04885) - 1056493..1057269 (+) 777 WP_005597402.1 TSUP family transporter -
  D1100_RS04875 (D1100_04890) nth 1057270..1057902 (+) 633 WP_005597404.1 endonuclease III -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49646.18 Da        Isoelectric Point: 7.9094

>NTDB_id=272344 D1100_RS04850 WP_017357656.1 1053031..1054410(+) (radA/sms) [Actinobacillus pleuropneumoniae strain WF83]
MAKAPKTAYVCNDCGAEYSRWVGQCKECKSWNTVSEIRLISSKEATKSDRFSGYAGETSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLSKDLPTLYVTGEESLQQVAMRANRLGLPTDNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLADIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHCIDASI
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSDEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRALPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIIPHGNAPKKPIKGMEVFTVKKLSDALDILGNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=272344 D1100_RS04850 WP_017357656.1 1053031..1054410(+) (radA/sms) [Actinobacillus pleuropneumoniae strain WF83]
ATGGCTAAAGCACCAAAAACAGCTTATGTATGTAATGATTGCGGCGCGGAATATTCTCGTTGGGTGGGGCAATGCAAAGA
ATGTAAGTCTTGGAATACCGTGAGTGAAATCAGACTTATCTCGAGTAAAGAAGCAACAAAAAGCGACCGCTTCAGCGGAT
ATGCCGGCGAAACCTCCGGTAAAGTTCAAACTTTATCCGAAATCAGCTTACAAGAAGTGCCTCGTTTTAGCAGCGGCTTT
AATGAATTGGATCGCGTACTGGGCGGCGGTGTCGTGCCGGGTTCGGCAATTTTAATCGGCGGCCATCCCGGTGCCGGTAA
ATCCACTCTGCTTTTACAAGTAATGTGCGGTTTGTCTAAAGATTTGCCGACCCTTTACGTGACTGGTGAAGAATCCCTAC
AACAAGTGGCGATGCGAGCGAATCGTTTAGGCTTACCGACCGACAATCTCAAGATGCTTTCAGAAACTTCGGTCGAGCAT
ATTTGTAATATCGCCGACCAAGAAAAGCCGAAAATTATGGTAATCGACTCAATTCAGGTCATGCACCTTGCCGATATTCA
ATCTTCTCCCGGTAGCGTTTCGCAAGTGCGGGAATGCGCTTCGTTTCTGACTCGCTATGCAAAAACCCGCCAAGTAGCGA
TTATTATGGTCGGACACGTCACCAAAGACGGGACACTTGCCGGCCCGAAAGTGTTGGAACACTGTATTGACGCCTCAATT
TTACTGGAAGGCGAATCGGACTCTCGATTCCGTACCTTACGCAGCCAGAAAAACCGTTTCGGTGCGGTCAATGAATTGGG
CGTATTTGCCATGACCGAACAAGGCTTACGAGAAGTAAAAAACCCTTCCGCGATTTTCCTCAGCCGTAGCGATGAACAAA
CGCCGGGCAGTTCCGTGATGGTGCTTTGGGAAGGAACCCGTCCGTTATTAGTCGAAATTCAAGCGTTGGTTGATCATTCG
ATGTTGGCTAATCCTCGCCGTGTCGCCGTAGGTTTAGATCACAACCGTTTATCGCTCTTACTTGCGGTGTTACATCGCCA
CGGCGGTTTGCAAATGTCGGATCAAGATGTGTTCGTCAATGTGGTCGGCGGGGTAAAAGTGACCGAGACCAGTGCCGATC
TCGCCCTAATTTTGGCGTTAATTTCCAGTTTTAGAAATCGTGCGTTACCGCAAGATTTGGTCGTATTCGGCGAGGTGGGA
TTAGCCGGAGAAATTCGTCCGGTACCGAGCGGTCAAGAACGTATCAGCGAAGCGGCGAAGCACGGTTTTAAACGTGCGAT
TATTCCTCACGGCAACGCACCTAAAAAACCGATTAAAGGTATGGAAGTCTTTACCGTTAAGAAACTAAGCGACGCACTAG
ATATTCTCGGCAATCTCTAA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(83-224)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

48.14

99.564

0.479
  radA Streptococcus mitis NCTC 12261

44.493

98.911

0.44
  radA Streptococcus mitis SK321

44.273

98.911

0.438
  radA Streptococcus pneumoniae Rx1

45.581

93.682

0.427
  radA Streptococcus pneumoniae D39

45.581

93.682

0.427
  radA Streptococcus pneumoniae R6

45.581

93.682

0.427
  radA Streptococcus pneumoniae TIGR4

45.581

93.682

0.427

Multiple sequence alignment