Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   C2I28_RS00990 Genome accession   NZ_CP026736
Coordinates   175664..176104 (+) Length   146 a.a.
NCBI ID   WP_028414988.1    Uniprot ID   A0AAX6BNS1
Organism   Priestia megaterium strain YC4-R4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 170664..181104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2I28_RS00970 (C2I28_00930) - 171068..171739 (-) 672 WP_028414985.1 response regulator transcription factor -
  C2I28_RS00975 (C2I28_00935) - 172065..172883 (+) 819 WP_028414986.1 squalene/phytoene synthase family protein -
  C2I28_RS00980 (C2I28_00940) - 172919..174868 (-) 1950 WP_028414987.1 FUSC family protein -
  C2I28_RS00985 (C2I28_00945) - 175025..175285 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  C2I28_RS00990 (C2I28_00950) nucA/comI 175664..176104 (+) 441 WP_028414988.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  C2I28_RS00995 (C2I28_00955) comJ 176140..176547 (+) 408 WP_098113593.1 competence protein ComJ -
  C2I28_RS01000 (C2I28_00960) - 176649..177239 (-) 591 WP_028414990.1 CPBP family intramembrane glutamic endopeptidase -
  C2I28_RS01005 (C2I28_00965) helD 177628..179946 (-) 2319 WP_108674279.1 RNA polymerase recycling motor HelD -
  C2I28_RS28115 - 180190..180315 (-) 126 WP_014458690.1 hypothetical protein -
  C2I28_RS01010 (C2I28_00970) - 180456..180971 (-) 516 WP_108674280.1 enoyl-CoA hydratase/isomerase family protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16034.86 Da        Isoelectric Point: 4.4411

>NTDB_id=272030 C2I28_RS00990 WP_028414988.1 175664..176104(+) (nucA/comI) [Priestia megaterium strain YC4-R4]
MSTYGKKLMASLFIIIACVLGFIGHAAFLAPEKTEAEANYDATLYFPLDRYPETGDHIRDAITAGHSSVCTIDRDGAEAN
REESLKGYPTKTGYDRDEWPMAMCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 441 bp        

>NTDB_id=272030 C2I28_RS00990 WP_028414988.1 175664..176104(+) (nucA/comI) [Priestia megaterium strain YC4-R4]
ATGAGTACATACGGGAAAAAATTAATGGCAAGTCTATTCATTATCATTGCATGCGTTTTAGGGTTTATAGGACATGCGGC
GTTTTTAGCTCCTGAAAAAACGGAAGCCGAAGCAAACTATGATGCAACGCTTTATTTTCCACTGGACCGATATCCTGAAA
CCGGCGATCATATTCGCGACGCAATTACTGCTGGTCACTCTTCCGTTTGCACAATTGATCGCGATGGTGCTGAAGCAAAC
CGAGAAGAATCGCTGAAAGGATACCCAACAAAAACAGGATATGACCGTGATGAATGGCCAATGGCTATGTGCGAAGAAGG
CGGAGCAGGAGCTGATATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGGAAATCAGTTAGAAC
AGTACCCAGATGGTACACGCGTACAATTTATCGTGCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

65.079

86.301

0.562

Multiple sequence alignment